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Shift of Propionate-Oxidizing Bacteria with HRT Decrease in an UASB Reactor Containing Propionate as a Sole Carbon Source

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Abstract

Propionate is a main intermediate product, and its degradation is crucial for maintaining the efficiency and stability of an anaerobic reactor. However, there was little information about the effects of ecological factor on propionate-oxidizing bacteria. In current research, microbial community composition and quantitative analysis of some identified propionate-oxidizing bacteria with hydraulic retention time (HRT) decrease in an upflow anaerobic sludge blanket (UASB) reactor containing propionate as sole carbon source was investigated. The results showed that propionate-oxidizing bacteria from Syntrophobacter, Pelotomaculum, and Smithella were major functional bacteria in this UASB system. Most propionate-oxidizing bacteria in composition have not changed with HRT decrease. However, the number of previously identified propionate-oxidizing bacteria from these three genera exhibited significant shift. Under HRT 10 h condition, Pelotomaculum schinkii was dominant and its quantity was 1.2 × 104 16S ribosomal RNA (rRNA) gene copies/ng DNA, occupying 56.2 % in total detectable propionate-oxidizing bacteria. HRT decrease from 10 h to 8 and 6 h stepwise resulted in P. schinkii, Syntrophobacter sulfatireducens and Smithella propionica becoming the main population. HRT decrease from 6 to 4 h did not markedly change the amount of propionate-oxidizing bacteria, but S. propionica dominated in the reactor.

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Abbreviations

UASB:

Upflow anaerobic sludge blanket

CSTR:

Completely stirred tank reactor

ABR:

Anaerobic baffled reactor

COD:

Chemical oxygen demand

VFAs:

Volatile fatty acids

PCR-DGGE:

Polymerase chain reaction-denaturing gradient gel electrophoresis

QPCR:

Quantitative polymerase chain reaction

HRT:

Hydraulic retention time

OLR:

Organic loading rate

MLVSS:

Mixed liquid volatile suspended solid

SCRR:

Specific COD removal rate

SMPR:

Specific methane production rate

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Acknowledgments

The authors gratefully acknowledge the National Natural Science Foundation of China (Grant No. 51178136), the Science and Technology Department of Heilongjiang Province (Grant No. GC13C303), and the State Key Laboratory of Urban Water Resource and Environment (HIT) (Grant No. 2013DX11) for valuable financial support.

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Authors and Affiliations

  1. State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150090, People’s Republic of China

    Qiaoying Ban & Jianzheng Li

  2. College of Environment and Resource, Shanxi University, Taiyuan, 030006, People’s Republic of China

    Qiaoying Ban & Liguo Zhang

Authors
  1. Qiaoying Ban
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  2. Liguo Zhang
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Correspondence to Jianzheng Li.

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Ban, Q., Zhang, L. & Li, J. Shift of Propionate-Oxidizing Bacteria with HRT Decrease in an UASB Reactor Containing Propionate as a Sole Carbon Source. Appl Biochem Biotechnol 175, 274–286 (2015). https://doi.org/10.1007/s12010-014-1265-8

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  • DOI: https://doi.org/10.1007/s12010-014-1265-8

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