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Pyrazole-Triazine Conjugate as Highly Selective and Sensitive Fluorescence Probe for Silver (I) Detection and Its Imaging in Living Cells

  • Chemistry and Physics
  • Published:
Wuhan University Journal of Natural Sciences

Abstract

A new fluorescence probe 2,2′-(6-(4-(diethylamino) phenyl)-1,3,5-triazine-2,4-diyl)bis(1H-pyrazole-1,3-diyl) diacetate (EATPA) based on 1, 3, 5-triazine was designed and synthesized. It exhibited distinct fluorescence quenching in the presence of silver ions that can be used for highly sensitive and selective detection of Ag+. Fluorescence titration and Job’s plot analysis revealed the formation of [Ag(EATPA)2]+ entity with high binding constant (1.43×104 L/mol) and low detection limit (0.882 µmol/L). Furthermore, live-cell imaging experiments demonstrated that EATPA is potentially applicable for the tracking of Ag+ in living cells.

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Authors and Affiliations

  1. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072, China

    Yanhong Liu, Bingqiong Yu, Qingya Zhu & Kun Yan

Authors
  1. Yanhong Liu
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  2. Bingqiong Yu
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  3. Qingya Zhu
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  4. Kun Yan
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Corresponding author

Correspondence to Kun Yan.

Additional information

Foundation item: Supported by the National Natural Science Foundation of China (21401144), the Natural Science Foundation of Hubei Province (2013CFB236), and the Independent Research Discipline Cross-funding of Wuhan University (2042017kf0215)

Biography: LIU Yanhong, female, Master candidate, research direction: fluorescent probes and their applications in live cell imaging.

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Liu, Y., Yu, B., Zhu, Q. et al. Pyrazole-Triazine Conjugate as Highly Selective and Sensitive Fluorescence Probe for Silver (I) Detection and Its Imaging in Living Cells. Wuhan Univ. J. Nat. Sci. 24, 409–416 (2019). https://doi.org/10.1007/s11859-019-1414-6

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  • DOI: https://doi.org/10.1007/s11859-019-1414-6

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