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A facile technique to prepare cross-linked enzyme aggregates of bovine pancreatic lipase using bovine serum albumin as an additive

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Abstract

Cross-linked enzyme aggregates from bovine pancreatic lipase were prepared by co-aggregation of lipase and BSA (Lipase-BSA-CLEAs). The main factors in the preparation of lipase-BSA-CLEAs were optimized. The highest activity recovery was around 75% under the condition of using 1% (v/v) glutaraldehyde as cross-linker and 0.05 g/L bovine serum albumin as feeder for 2 h cross linking. The optimum temperature for both lipase-CLEAs and lipase- BSA-CLEAs was measured as 60 °C, which is 10 °C higher than that of free lipase. Moreover, the lipase-BSA-CLEAs evidenced higher thermal stability and excellent reusability in comparison with the lipase-CLEAs. Lipase-BSA-CLEAs retained more than 75% of the initial activity after eight cycles of reuse, while lipase-CLEAs only retained 20% of its initial activity. Additionally, lipase-BSA-CLEAs showed more storage stability than free lipase and lipase-CLEAs. The high stability and recyclability of lipase-BSA-CLEAs make it efficient for different industrial applications.

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Authors and Affiliations

  1. Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, 70 Yuhua East Road, Shijiazhang, 050018, P. R. China

    Jian Dong Cui, Rong Lin Liu & Lin Bo Li

  2. Tianjin Key Laboratory of Food-Biotechnology, Tianjin University of Commerce, Beichen District, Tianjin, 300134, P. R. China

    Jian Dong Cui

  3. Key Laboratory of Industry Microbiology, Ministry of Education, Tianjin University of Science and Technology, 29 Thirteenth Street, Tai Da Development Area, Tianjin, 300457, P. R. China

    Jian Dong Cui

Authors
  1. Jian Dong Cui
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  2. Rong Lin Liu
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  3. Lin Bo Li
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Correspondence to Jian Dong Cui.

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Cui, J.D., Liu, R.L. & Li, L.B. A facile technique to prepare cross-linked enzyme aggregates of bovine pancreatic lipase using bovine serum albumin as an additive. Korean J. Chem. Eng. 33, 610–615 (2016). https://doi.org/10.1007/s11814-015-0190-z

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  • DOI: https://doi.org/10.1007/s11814-015-0190-z

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