Abstract
Pacific oyster (Crassostrea gigas) is one of the most important mollusks cultured all around the world. Selective breeding programs of Pacific oysters in China is initiated since 2006 and developed the genetically improved strain with fast-growing trait. However, little is known about the metabolic signatures of the fast-growing trait. In the present study, the non-targeted metabolomics was performed to analyze the metabolic signatures of adductor muscle tissue in one-year old Pacific oysters from fast-growing strain and the wild population. A total of 7767 and 10174 valid peaks were extracted and quantified in ESI+ and ESI− modes, resulting in 399 and 381 annotated metabolites, respectively. PCA and OPLS-DA revealed that considerable separation among samples from fast-growing strain and wild population, suggesting the differences in metabolic signatures. Meanwhile, 81 significantly different metabolites (SDMs) were identified in the comparisons between fast-growing strain and wild population, based on the strict thresholds. It was found that there were highly correlation and conserved coordination among these SDMs. KEGG enrichment analysis indicated that the SDMs were tightly related to pantothenate and CoA biosynthesis, steroid hormone biosynthesis, riboflavin metabolism, and arginine and proline metabolism. Of them, the CoA biosynthesis and metabolism, affected by pantetheine and pantothenic acid, might be important for the growth of Pacific oysters under artificial selective breeding. The study provides the comprehensive views of metabolic signatures in response to artificially selective breeding, and is helpful to better understand the molecular mechanism of fast-growing traits in Pacific oysters.
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This research was supported by grants from the Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province (Nos. 2021ZLGX03 and 2022LZGCQY010), and the China Agriculture Research System Project (No. CARS-49).
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Hu, B., Tian, Y., Liu, S. et al. Non-Targeted Metabolomics Reveals the Metabolic Alterations in Response to Artificial Selective Breeding in the Fast-Growing Strains of Pacific Oyster. J. Ocean Univ. China 23, 518–528 (2024). https://doi.org/10.1007/s11802-024-5717-7
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DOI: https://doi.org/10.1007/s11802-024-5717-7