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UPLC-MS metabolomics provides insights into the differences between black- and white-shelled Pacific oysters Crassostrea gigas

  • Aquaculture and Fisheries
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Abstract

A variety of shell colors are one of the most fundamental characteristics of molluscs, which have importantly ecological and economic significance. The Pacific oyster Crassostrea gigas is distributed in many sea areas around the world and also an aquacultured mollusc with high nutritional value. In this study, the whole soft body and the mantle tissue of black-shelled Pacific oyster (BSO) and white-shelled Pacific oyster (WSO) with starkly different melanin contents were compared, and the differences in physiology and metabolism between BSO and WSO were analyzed. The results of physiological indicators suggested BSO show more melanin, more dry matter, more crude lipid content, and stronger ability to scavenge free radicals than WSO. The altered metabolites of glycerophospholipids, fatty acyls, and steroids revealed different regulatory mechanisms of lipids. The correlation analysis of metabolomics and previously published RNAseq data suggested that BSO and WSO mainly differed in the basal metabolic processes, such as lipid, amino acid and purine metabolisms. This study provides insights into the changes in the physiological indictors and the metabolites of oysters with varying melanin content.

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Data Availability Statement

The MS raw data that support the findings of this study are available in figshare with the identifier. Dataset, https://doi.org/10.6084/m9.figshare.12261065.v1.

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Authors and Affiliations

  1. School of Agriculture, Ludong University, Yantai, 264025, China

    Xi Chen, Qiuyun Jiang, Hongce Song, Lingling Li, Chaoyi Xie, Baoyu Huang, Yaqiong Liu, Meiwei Zhang, Lei Wei & Xiaotong Wang

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  1. Xi Chen
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  2. Qiuyun Jiang
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  3. Hongce Song
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Correspondence to Lei Wei or Xiaotong Wang.

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Supported by the Agricultural Variety Improvement Project of Shandong Province (No. 2019LZGC020), the National Natural Science Foundation of China (Nos. 41906088, 41876193, 31802328), the National Key R&D Program of China (No. 2018YFD0901400), the Special Funds for Taishan Scholars Project of Shandong Province, China (No. tsqn201812094), the Shandong Provincial Natural Science Foundation, China (No. ZR2019MC002), the Modern Agricultural Industry Technology System of Shandong Province, China (No. SDAIT-14-03), and the Plan of Excellent Youth Innovation Team of Colleges and universities in Shandong Province, China (No. 2019KJF004)

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Chen, X., Jiang, Q., Song, H. et al. UPLC-MS metabolomics provides insights into the differences between black- and white-shelled Pacific oysters Crassostrea gigas. J. Ocean. Limnol. 39, 340–349 (2021). https://doi.org/10.1007/s00343-020-0117-3

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