Abstract
Rheumatoid arthritis is a systemic, chronic disease which may increase the risk of osteoporosis. This study was carried out in order to examine the effect of conjugated linoleic acid (CLA) on bone markers in rheumatoid arthritis disease which is the most common autoimmune disease. The present study is a randomized double-blind clinical trial. Subjects included 52 patients with active rheumatoid arthritis who were divided into two groups. Group I received standard treatment plus 2 daily 1.25 g capsules (Containing about 2 g of 9-cis 11-trans isomer and 10-cis 12-trans isomer in ratio of 50 −50 CLA in glycerinated form), Group II received standard treatment plus 2 Placebo 1.25 g capsules containing sunflower oil with high oleic acid. Telopeptides C, osteocalcin, and MMP3 were analyzed by ELISA method, PGE2 was done by competitive enzymatic immunoassay method, and IGF-1 was analyzed by the IRMA method based on the sandwich method and ALK-P of bone. Before and after the intervention, the questionnaires about general information, nutrition assessment and medical history were filled out by the subjects. Nutritional assessment was done by a 24-h record questionnaire for the three-day diet. The results were analyzed using SPSS software (version 18). Findings: There was no significant difference between the groups in enzyme activity of ALK-P of bone, PGE2 and MMP3 variables. However, differences between the two groups in terms of activity of telopeptides C, Osteocalcin, and IGF1 were significant (P < 0.05). CLA has a potentially beneficial effect on bone markers in patients with rheumatoid arthritis. Therefore, in order to study the effect of CLA on bone health in patients with RA and all patients with autoimmune and bone diseases more studies with longer duration and evaluation of bone mass density are required.
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Abbreviations
- ACR:
-
American College of Rheumatology
- ALK-P:
-
Alkaline phosphatase
- BMD:
-
Bone mass density
- BMI:
-
Body mass index
- BMP:
-
Bone morphogenic proteins
- CLA:
-
Conjugated linoleic acid
- FA:
-
Fatty acid
- IGF1:
-
Insulin-like growth factor 1
- MMP3:
-
Matrix metalloproteinase 3
- PGE2 :
-
Prostaglandine E2
- PPARγ:
-
Peroxisome proliferator activated receptor gamma
- RA:
-
Rheumatoid Arthritis
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Acknowledgements
This study was funded by a research grant from the Research Deputy of Tehran University of Medical Sciences (with Proposal No. 24528). We are thankful for the kind cooperation of Lipid Nutrition Company (Netherlands) for providing CLA capsules as Clarinol G-80 and its placebo for this research.
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The study was funded by the Iran University of Medical Sciences. This study was conducted independently from the funders. The funders were not involved in the study design, data collection and analysis, interpretation of the data or in the writing of the report or its submission for publication.
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The study was approved by the respective research ethics committees and medicinal regulatory agencies in each country (No IRCT201207109472N4). Informed written consent was obtained from the patient before recruitment.
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DP affirms that the manuscript is an honest, accurate, and transparent account of the study; that no important aspects of the study have been omitted; and that any discrepancies from the study as planned have been explained.
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Aryaeian, N., Shahram, F. & Djalali, M. CLA Has a Useful Effect on Bone Markers in Patients with Rheumatoid Arthritis. Lipids 51, 1397–1405 (2016). https://doi.org/10.1007/s11745-016-4201-6
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DOI: https://doi.org/10.1007/s11745-016-4201-6