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Mitigating drought stress by plant-derived biostimulant in Arbequina olive (Olea europeae L.) cultivar conducted in super high density

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Abstract

The climate change has negative effects on crop production worldwide. The scarcity of water in semi-arid zones proper the use of biostimulants to improve yield and quality parameters. The present work evaluated the impact of exogenous spraying with moringa leaves aqueous extract (MLE 5%) on agro-physiological behavior of the olive cultivar “Arbequina” conducted in super high density during two growing seasons (2019–2020). The experiment consisted of six treatments (T0 = 100% ETc; T1 = 100% ETc + 5% MLE; T2 = 75% ETc; T3 = 75% ETc + 5% MLE; T4 = 50% ETc; and T5 = 50% ETc + 5% MLE). Twelve trees were considered for each treatment and distilled water was applied as control for the MLE treatment. Vegetative growth, yield, water status, antioxidants, oil parameters, leaf gas exchange, and chlorophyll fluorescence were analyzed. Results showed that the net photosynthesis (Pn), stomatal conductance (gs), and evapotranspiration (E) varied significantly in response to drought stress and MLE treatments. The two water regimes (100% and 75% ETc) showed similar behavior regarding the agro-physiological responses to the applied treatments, whereas the 50% ETc water regime significantly affected the studied parameters. The MLE was effective in mitigating the water stress on olive trees, by improving vegetative growth, yield, photosynthesis, and water productivity. It appears that trees grown under 75% ETc water regime were more responsive to 5% MLE, as they exhibit the highest yield (8.4 kg/tree), oil content (28.3%), and water use efficiency, whereas 25% of irrigation water was saved.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

References

  • Abboud S, Dbara S, Abidi W, Braham M (2019) Differential agro-physiological responses induced by partial root-zone drying irrigation in olive cultivars grown in semi-arid conditions. Environ Exp Bot 167:103863. https://doi.org/10.1016/j.envexpbot.2019.103863

    Article  CAS  Google Scholar 

  • Abboud S, Vives-Peris V, Dbara S, Gomez-Cadenas A, Perez-Clemente RM, Abidi W, Braham M (2021) Water status, biochemical and hormonal changes involved in the response of Olea europaea L. to water deficit induced by partial root-zone drying irrigation (PRD). SciHort 276:109737. https://doi.org/10.1016/j.scienta.2020.109737

    Article  CAS  Google Scholar 

  • Abd El-Mageed TA, Semida WM, Rady MM (2017) Moringa leaf extract as biostimulant improves water use efficiency, physio-biochemical attributes of squash plants under deficit irrigation. Agri Water Manag 193:46–54. https://doi.org/10.1016/j.agwat.2017.08.004

    Article  Google Scholar 

  • Ahumada-Orellana LE, Ortega-Farías S, Searles PS, Retamales JB (2017) Yield and water productivity responses to irrigation cut-off strategies after fruit set using stem water potential thresholds in a super-high density olive orchard. Front Plant Sci 8:1280

    Article  PubMed  PubMed Central  Google Scholar 

  • Allen RG, Pereira LS, Raes D, Smith M (1998) Crop evapotranspiration guidelines for computing crop water requirements. Irrig Drain Paper 56. FAO, Rome, 300

  • Ashfaq M, Basra S, Ashfaq U (2012) Moringa: a miracle plant of agro-forestry. J Agri Soc Sci 8:115–122

    Google Scholar 

  • Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59:206–216. https://doi.org/10.1016/j.envexpbot.2005.12.006

    Article  CAS  Google Scholar 

  • Bates LS, Waldeen RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Battacharyya D, Babgohari MZ, Rathor P, Prithiviraj B (2015) Seaweed extracts as biostimulants in horticulture. Sci Hortic 196:39–48

    Article  CAS  Google Scholar 

  • Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol 28:25–30

  • Brito C, Dinis LT, Moutinho-Pereira J, Correia CM (2019) Drought stress effects and olive tree acclimation under a changing climate. Plants 8:232. https://doi.org/10.3390/plants8070232

  • Brown P, Saa S (2015) Biostimulants in agriculture. Front Plant Sci 6:671. https://doi.org/10.3389/fpls.2015.00671

    Article  PubMed  PubMed Central  Google Scholar 

  • Bulgari R, Cocetta G, Trivellini A, Vernieri P, Ferrante A (2015) Biostimulants and crop responses: a review. Biol Agric Hortic 31:1–17

    Article  Google Scholar 

  • Candido V, Boari F, Cantore V, Castronuovo D, Denora M, Sergio L, Todorovic M, Schiattone MI (2023) Interactive effect of water regime, nitrogen rate and biostimulant application on physiological and biochemical traits of wild rocket. Agric Water Manag 277:108075. https://doi.org/10.1016/j.agwat.2022.108075

    Article  Google Scholar 

  • Cirillo A, Conti S, Graziani G, El-Nakhel C, Rouphael Y, Ritieni A, Di Vaio C (2021) Mitigation of high-temperature damage by application of kaolin and pinolene on young olive trees (Oleaeuropaea L.): a preliminary experiment to assess biometric, eco-physiological and nutraceutical parameters. Agronomy 11:1884. https://doi.org/10.3390/agronomy11091884

    Article  CAS  Google Scholar 

  • Conde-Innamorato P, García C, Villamil JJ, Ibáñez F, Zoppolo R, Arias-Sibillotte M, Ponce De León I, Borsani O, García-Inza GP (2022) The impact of irrigation on olive fruit yield and oil quality in a humid climate. Agronomy 12:313. https://doi.org/10.3390/agronomy12020313

    Article  CAS  Google Scholar 

  • Dalal A, Bourstein R, Haish N, Shenhar I, Wallach R, Moshelion M (2019) Dynamic physiological phenotyping of drought-stressed pepper plants treated with “productivity-enhancing” and “survivability enhancing” biostimulants. Front Plant Sci 10:905. https://doi.org/10.3389/fpls.2019.00905

    Article  PubMed  PubMed Central  Google Scholar 

  • Del Buono D, Regni L, Del Pino AM, Bartucca ML, Palmerini CA, Proietti P (2021) Effects of Megafol on the olive cultivar ‘Arbequina’ grown under severe saline stress in terms of physiological traits, oxidative stress, antioxidant defenses, and cytosolic Ca2+. Front Plant Sci 11:603576

    Article  PubMed  PubMed Central  Google Scholar 

  • Dias MC, Araújo M, Silva S, Santos C (2022) Sustainable olive culture under climate change: the potential of biostimulants. Horticulturae 8:1048. https://doi.org/10.3390/horticulturae8111048

    Article  Google Scholar 

  • Diaz-Espejo A, Nicolas E, Fernandez JE (2007) Seasonal evolution of diffusional limitations and photosynthetic capacity in olive under drought. Plant Cell Envir 30:922–933

    Article  CAS  Google Scholar 

  • El Yamani M, Sakar EH, Boussakouran A, Rharrabti Y (2019) Physiological and biochemical responses of young olive trees (Olea europaea L.) to water stress during flowering. Arch Biol Sci 71(1):123–132. https://doi.org/10.2298/ABS181001054E

    Article  Google Scholar 

  • Fereres E, Castel JR (1981) Drip irrigation management. Division of agricultural 480 sciences. University of California (Publication Leaflet 21259)

  • Frioni T, Sabbatini P, Tombesi S, Norrie J, Poni S, Gatti M, Palliotti A (2018) Effects of a biostimulant derived from the brown seaweed Ascophyllum nodosum on ripening dynamics and fruit quality of grapevines. Sci Hortic 232:97–106

    Article  Google Scholar 

  • Gholami R, Hoveizeh NF, Zahedi SM, Gholami H, Carillo P (2022) Melatonin alleviates the adverse effects of water stress in adult olive cultivars (Olea europea cv. Sevillana & Roughani) in field condition. Agri Water Manag 269:107681. https://doi.org/10.1016/j.agwat.2022.107681

    Article  Google Scholar 

  • Gonçalves A, Silva E, Brito C, Martins S, Pinto L, Dinis LT, Luzio A, Martins-Gomes C, Fernandes-Silva A, Ribeiro C, Rodrigues MÂ, Moutinho-Pereira J, Nunesc FM, Correia C (2020) Olive tree physiology and chemical composition of fruits are modulated by different deficit irrigation strategies. J Sci Food Agric 100:682–694

    Article  PubMed  Google Scholar 

  • Grattan SR, Berenquer MJ, Connell JH, Polito VS, Vossen PM (2006) Olive oil production as influenced by different quantities of applied water. Agr Water Manag 85:133–140

    Article  Google Scholar 

  • Gucci R, Lombardini L, Tattini M (1997) Analysis of leaf water relations in leaves of two olive (Oleaeuropaea) cultivars differing in tolerance to salinity. TreePhysiol 17:13–21

    CAS  Google Scholar 

  • Gurav RG, Jadhav JP (2013) A novel source of biofertilizer from feather biomass for banana cultivation. Environ SciPollut R 20:4532–4539. https://doi.org/10.1007/s11356-012-1405-z

    Article  CAS  Google Scholar 

  • Gutiérrez-Gamboa G, Romanazzi G, Garde-Cerdán T, Pérez-Álvarez EP (2019) A review of the use of biostimulants in the vineyard for improved grape and wine quality: Effects on prevention of grapevine diseases. J Sci Food Agric 99:1001–1009. https://doi.org/10.1002/jsfa.9353

    Article  CAS  PubMed  Google Scholar 

  • Hassanein RA, Abdelkader AF, Faramawy HM (2019) Moringa leaf extracts as biostimulants-inducing salinity tolerance in the sweet basil plant. Egypt J Bot 59(2):303–318

    Google Scholar 

  • Hernández ML, Velázquez-Palmero D, Sicardo MD, Fernández JE, Diaz-Espejo A, Martínez-Rivas JM (2018) Effect of a regulated deficit irrigation strategy in a hedgerow ‘Arbequina’ olive orchard on the mesocarp fatty acid composition and desaturase gene expression with respect to olive oil quality. Agric Water Manag 204:100–106

    Article  Google Scholar 

  • Hueso A, Trentacoste ER, Junquera P, Gómez-Miguel V, Gómez-del-Campo M (2019) Differences in stem water potential during oil synthesis determine fruit characteristics and production but not vegetative growth or return bloom in an olive hedgerow orchard (cv. Arbequina). Agric Water Manag 223:105589

    Article  Google Scholar 

  • Iniesta F, Testi L, Orgaz F, Villalobos FJ (2009) The effects of regulated and continuous deficit irrigation on the water use, growth and yield of olive trees. Eur J Agron 30:258–265

    Article  Google Scholar 

  • IOC (2015) International Olive Council. https://www.internationaloliveoil.org/. Accessed 15 Oct 2020

  • Kim DO, Jeong SW, Lee CY (2003) Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem 81(3):321–326. https://doi.org/10.1016/S0308-8146(02)00423-5

    Article  CAS  Google Scholar 

  • Leogrande R, El Chami D, Fumarola G, Di Carolo M, Piegari G, Elefante M, Perrelli D, Dongiovanni C (2022) Biostimulants for resilient agriculture: a preliminary assessment in Italy. Sustainability 14:6816. https://doi.org/10.3390/su14116816

    Article  CAS  Google Scholar 

  • Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 11(5):591–592. https://doi.org/10.1042/bst0110591

    Article  CAS  Google Scholar 

  • Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Meth Enzymol 148(C):350–382. https://doi.org/10.1016/0076- 6879(87)48036–1

  • Little HA, Spann TM (2010) Commercial extracts of Ascophyllum nodosum increase growth and improve water status of potted citrus rootstocks under deficit irrigation. Hortscience 45:S63

    Google Scholar 

  • Min Z, Li R, Chen L, Zhang Y, Li Z, Liu M, Ju Y, Fang Y (2019) Alleviation of drought stress in grapevine by foliar-applied strigolactones. Plant Physiol Biochem 135:99–110. https://doi.org/10.1016/j.plaphy.2018.11.037

    Article  CAS  PubMed  Google Scholar 

  • Mínguez-Mosquera MI, Gandul-Rojas B, Montaño-Asquerino A, Garrido-Fernández J (1991) Determination of chlorophylls and carotenoids by HPLC during olive lactic fermentation. J Chromatogr 585:259–266

    Article  Google Scholar 

  • Mullins MG (2006) Plant Improvement in Horticulture: The Case for Fruit Breeding. The Regional Institute, pp. 85–92

  • Peñalver R, Martínez-Zamora L, Lorenzo JM, Ros G, Nieto G (2022) Nutritional and antioxidant properties of moringaoleifera leaves in functional foods. Foods 11:1107. https://doi.org/10.3390/foods11081107

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Petridis A, Therios I, Samouris G, Koundouras S, Giannakoula A (2012) Effect of water deficit on leaf phenolic composition, gas exchange, oxidative damage and antioxidant activity of four Greek olive (Olea europaea L.) cultivars. Plant Physiol Biochem 60:1–11. https://doi.org/10.1016/J.PLAPHY.2012.07.014

    Article  CAS  PubMed  Google Scholar 

  • Regni L, Del Buono D, Miras-Moreno B, Senizza B, Lucini L, Trevisan M, MorelliVenturi D, Costantino F, Proietti P (2021) Biostimulant effects of an aqueous extract of duckweed (Lemna minor L.) on physiological and biochemical traits in the olive tree. Agriculture 11:1299. https://doi.org/10.3390/agriculture11121299

    Article  CAS  Google Scholar 

  • Robyt JF, White BJ (1987) Biochemical technique, theory and practice. Brooks/Cole Publishing Company, Monterrey, p 407

    Google Scholar 

  • Ruzzi M, Aroca R (2015) Plant growth-promoting rhizobacteria act as biostimulants in horticulture. Sci Hortic 196:124–134. https://doi.org/10.1016/J.Scienta.2015.08.042

    Article  CAS  Google Scholar 

  • Samad M, Sajid M, Hussain I, Samad N, Jan N (2019) Influence of herbal extract and storage duration on fruit quality of China lime. Horticult Int J 3(3):153–158. https://doi.org/10.15406/hij.2019.03.00123

    Article  Google Scholar 

  • Shackel K, Ahmadi AH, Biasi W (1997) Plant water status as an index of irrigation need in deciduous fruit trees. HortTechnology 7(1):23–29

    Article  Google Scholar 

  • Skerget M, Kotnik P, Hadolin M, Hras A, Simonic M, Knez Z (2005) Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chem 89:191–198. https://doi.org/10.1016/j.foodchem.2004.02.025

    Article  CAS  Google Scholar 

  • Soppelsa S, Kelderer M, Casera C, Bassi M, Robatscher P, Andreotti C (2018) Use of biostimulants for organic apple production: effects on tree growth, yield, and fruit quality at harvest and during storage. Front Plant Sci 9:1342

    Article  PubMed  PubMed Central  Google Scholar 

  • Tanou G, Ziogas V, Molassiotis A (2017) Foliar nutrition, biostimulants and prime-like dynamics in fruit tree physiology: New insights on an old topic. Front Plant Sci 8:75. https://doi.org/10.3389/fpls.2017.00075

    Article  PubMed  PubMed Central  Google Scholar 

  • Van Kooten O, Snel JFH (1990) The use of chlorophyll fluorescence nomenclature in plant stress physiology. Photosynth Res 25(3):147–150

    Article  PubMed  Google Scholar 

  • Wahbi S, Wakrim R, Aganchich B, Tahi H, Serraj R (2005) Effects of partial rootzone drying (PRD) on adult olive tree (Olea europaea) in field conditions under arid climate: I. Physiological and agronomic responses. Agric Ecosyst Environ 106(2–3):289–301. https://doi.org/10.1016/J.AGEE.2004.10.015

    Article  Google Scholar 

  • Yasmeen A, Nouman W, Basra SMA, Wahid A, Hussain N, Afzal I (2014) Morphological and physiological response of tomato (Solanumlycopersicum L.) to natural and synthetic cytokinin sources: a comparative study. Acta Physiol Plant 36:3147–3155. https://doi.org/10.1007/s11738-014-1662-1

    Article  CAS  Google Scholar 

  • Yasmeen A (2011) Exploring the potential of moringa (Moringaoleifera) leaf extract as natural plant growth enhancer (Doctoral dissertation; University of Agriculture, Faisalabad, Pakistan). Retrieved from http://docplayer.net/21758964-Exploring-the-potential-of-moringa-moringa-oleifera-leaf-extract-as-natural-plant-growth-enhancer.html

  • Younis A, Akhtar MS, Riaz A, Zulfiqar F, Qasim M, Farooq A, Tariq U, Ahsan M, Bhatti ZM (2018) Improved cut flower and corm production by exogenous moringa leaf extract application on gladiolus cultivars. Acta Sci Pol 17:25–38. https://doi.org/10.24326/asphc.2018.4.3

    Article  Google Scholar 

  • Zulfiqar F, Allaire SE, Akram NA, Mendez A, Younis A, Peerzada AM, Shaukat N, Wright SR (2019) Challenges in organic component selection and biochar as an opportunity in potting substrates. J Plant Nutr 42:1386–1401. https://doi.org/10.1080/01904167.2019.1617310

    Article  CAS  Google Scholar 

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Acknowledgements

This study was supported by the Regional Center of Agriculture Research of SidiBouzid, Tunisia. The authors thank Rchid Guedri, Mohamed Ali Guedri, Walid Jlali, Adel Guedri, and Donya Ammari for technical assistance and support.

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  1. Regional Center of Agricultural Research of Sidi Bouzid (CRRA) PB 357, 9100, Sidi Bouzid, Tunisia

    Walid Abidi & Rawaa Akrimi

  2. Higher Institute of Agronomy of Chott Mariem, Sousse, Tunisia

    Mohamed Gouiaa

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Abidi, W., Akrimi, R. & Gouiaa, M. Mitigating drought stress by plant-derived biostimulant in Arbequina olive (Olea europeae L.) cultivar conducted in super high density. Acta Physiol Plant 45, 132 (2023). https://doi.org/10.1007/s11738-023-03613-9

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