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Synergistic action of indole acetic acid with homobrassinolide in easing the NaCl-induced toxicity in Solanum melongena L. seedlings

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Abstract

In the current work, the influence of indole-3-acetic acid (IAA; 10–5 M) and 28-homobrassinolide (HBL; 10–8 M) alone or in combination was explored in Solanum melongena L. seedlings treated with sodium chloride (NaCl). The NaCl at different concentrations (5 mM and 15 mM) reduced growth attributes, photosynthetic pigments as well as photosynthesis, and enhanced the dark respiratory rate, and these effects were accompanied with Na+ and reactive oxygen species (ROS) accumulation in leaf tissues. Moreover, NaCl reduced the proficiency of PS II with the increasing doses of selected stress which is apparent from the declined values of Fv/Fm (Phi_P0), Ѱ0 (Psi_0), ϕE0 (Phi_E0) and PIABS, and augmented values of energy fluxes: ABS/RC, ET0/RC, TR0/RC and DI0/RC. The NaCl treatment caused substantial reduction in nitrate and nitrite contents, activities of nitrate reductase, nitrite reductase, glutamine synthetase and glutamate synthase, and increased ammonium content and glutamate dehydrogenase activity. Enhanced level of ROS, i.e. superoxide radical (O2•−) and hydrogen peroxide (H2O2) under NaCl stress amplified the lipid peroxidation product malondialdehyde (MDA), in spite of considerable upsurge in performance of superoxide dismutase, peroxidase, catalase, glutathione-S-transferase enzymes and amount of non-protein thiol, cysteine and proline. Exogenous supplementation of IAA and HBL considerably assuaged the NaCl-induced collapse in growth attributes by manipulating the structural and functional features of photosynthetic apparatus. The ameliorating effect of IAA and HBL, in combination, was due to (1) substantial decrease in Na+ accumulation, (2) improvement in PS II efficiency and nitrogen metabolism and (3) reduced oxidative damage in S. melongena L. due to stimulatory response of antioxidants that keeps the level of oxidants under limit in the seedlings. Thus, the overall results campaigned that IAA and HBL act synergistically to overcome the toxic effects that NaCl had on S. melongena L. seedlings.

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Acknowledgements

Aman Deep Raju is grateful to the CSIR-UGC (JRF; letter no. 2061631013) for getting financial assistance as SRF to conduct the present piece of work. Professor Sheo Mohan Prasad is also thankful to the Department of Science and Technology (DST), New Delhi, for getting research amenities under DST‒FIST program.

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  1. Jitendra Kumar

    Present address: Institute of Engineering and Technology, Dr. Shakuntala, Misra National Rehabilitation University, Mohaan Road, Lucknow, U.P., 226017, India

  2. Aman Deep Raju, Parul Parihar and Rachana Singh contributed equally.

Authors and Affiliations

  1. Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Prayagraj, U.P., 211002, India

    Aman Deep Raju, Parul Parihar, Rachana Singh, Jitendra Kumar & Sheo Mohan Prasad

  2. School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Jalandhar, Punjab, India

    Parul Parihar

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Raju, A.D., Parihar, P., Singh, R. et al. Synergistic action of indole acetic acid with homobrassinolide in easing the NaCl-induced toxicity in Solanum melongena L. seedlings. Acta Physiol Plant 42, 68 (2020). https://doi.org/10.1007/s11738-020-03054-8

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