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Effects of Sleeve Gastrectomy on Serum 12α-Hydroxylated Bile Acids in a Diabetic Rat Model

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Abstract

Background

Diabetes mellitus is a prevalent disease that endangers human health. Bariatric surgery can effectively relieve insulin resistance with elevated serum bile acids (BAs). 12α-Hydroxylated BAs were previously reported to be associated with insulin resistance. The aim of this study was to analyze changes in 12α-hydroxylated BA composition and possible associated mechanisms in diabetic rats following sleeve gastrectomy (SG).

Methods

SG and sham operations were performed in diabetic rats induced by high-fat diet feeding and streptozotocin. Body weight, food intake, oral glucose tolerance test (OGTT), insulin tolerance test (ITT), and serum BAs were analyzed at corresponding time points. Cholesterol 12α-hydroxylase (CYP8B1) and transcription factor V-Maf Avian Musculoaponeurotic Fibrosarcoma Oncogene Homolog G (MAFG) expression levels were assessed by RT-PCR and western blotting.

Results

Compared with the SHAM group, the SG group displayed significant weight loss from 6 weeks postoperatively, accompanied by decreased food intake from 4 weeks after the operation. At 2 and 12 weeks postoperatively, the areas under the curve of OGTT and ITT were significantly decreased in the SG group. At 12 weeks post-operation, the SG group displayed elevated serum BAs, but the percentage of 12α-hydroxylated BAs was reduced. Furthermore, SG rats exhibited higher MAFG and lower CYP8B1 protein and mRNA levels in the liver (P < 0.05).

Conclusion

The percentage of 12α-hydroxylated bile acids was reduced after SG, which was relevant with the inhibition of CYP8B1 and overexpression of MAFG. These outcomes may play an important role in the improvement of insulin sensitivity following SG.

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Author information

Author notes

  1. Minggang Wang and Qunzheng Wu contributed equally to this work.

Authors and Affiliations

  1. Department of Hernia and Abdominal Wall Surgery, Beijing Chao-Yang Hospital, Capital Medical University, 100043, Beijing, People’s Republic of China

    Minggang Wang

  2. Department of General Surgery, Qilu Hospital of Shandong University, 107#, Wenhua Xi Road, Jinan, 250012, Shandong, People’s Republic of China

    Qunzheng Wu, Haibin Xie, Yi Shao, Mingwei Zhong, Xiang Zhang, Shaozhuang Liu, Sanyuan Hu & Guangyong Zhang

  3. Department of Cardiology, Affiliated Hospital of Taishan Medical University, Taian, 271000, Shandong, People’s Republic of China

    Xiao He

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  1. Minggang Wang
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  2. Qunzheng Wu
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Corresponding author

Correspondence to Guangyong Zhang.

Ethics declarations

All study protocols were approved by the Animal Care and Utilization Committee of Shandong University.

Conflict of Interest

The authors declare that they have no conflict of interest.

Statement of Human and Animal Rights

All applicable institutional and national guidelines for the care and use of animals were followed.

Funding

The National Natural Science Foundation of China (no. 81270888/H0713, no. 81370496/H0308) and the Fundamental Research Funds of Shandong University (no. 2014QLKY22).

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Wang, M., Wu, Q., Xie, H. et al. Effects of Sleeve Gastrectomy on Serum 12α-Hydroxylated Bile Acids in a Diabetic Rat Model. OBES SURG 27, 2912–2918 (2017). https://doi.org/10.1007/s11695-017-2714-6

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  • DOI: https://doi.org/10.1007/s11695-017-2714-6

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