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Comparative analysis of the physicochemical properties and biological activities of Ziziphus Jujuba cv. Goutouzao polysaccharides obtained by fractional precipitation

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Abstract

Polysaccharides are the main active constituents of Jujuba, yet there are few researches on Ziziphus Jujuba cv. Goutouzao polysaccharides. Traditional separation and purification method of Jujuba polysaccharides was ion-exchange column chromatography, which is complicated and time-consuming. In this study, four polysaccharide components (SJP-1, SJP-2, SJP-3, and SJP-4) were obtained from crude Ziziphus Jujuba cv. Goutouzao polysaccharides (SJP) by ethanol fractional precipitation which are higher convenience than traditional method. The physicochemical properties, immune activities, and antioxidant activities (free radical scavenging ability, reducing power, lipid peroxidation, metal chelating power, inhibition of protein oxidative damage, protective effects of HL-7702 cell damage) of SJP and SJP-1–SJP-4 were investigated to determine its major active constituent. These results showed that SJP-4 had the highest antioxidant and immune activities, which could be related to its monosaccharide composition (Ara, 29.5%; Man, 10.0%; Glc, 16.9%; and Gal, 28.2%) and low molecular weight (0.73 × 104 Da). These results provide a scientific basis for the comprehensive development and utilization of Ziziphus Jujuba cv. Goutouzao in the food, medicine, and health product industry.

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Abbreviations

RQP1d:

Fructus Jujubae polysaccharides fraction

EDTA:

Ethylene diamine tetraacetie acid

DMEM:

Dulbecco's modified eagle medium

DEAE-cellulose:

Diethylaminoethyl cellulose

BSA:

Bovine serum albumin

ZP2:

Jujube fruits polysaccharide

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Acknowledgements

This study was supported by National Natural Science Foundation of China (Nos. 31901694, 31972024, 32001697 and 31870798), Shaanxi Province Innovation Capability Support Plan-Science and Technology Innovation Team (2020TD-044).

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Author notes

  1. Tingting Liu and Wenqi Huang have contributed equally to this work.

Authors and Affiliations

  1. College of Life Sciences, Northwest University, Xi’an, 710069, China

    Tingting Liu, Linjuan Huang & Zhongfu Wang

  2. Infinitus (China) Company Ltd, Guangzhou, 510000, Guangdong, China

    Xiaoliang Lin

  3. Shaanxi Natural Carbohydrate Resource Engineering Research Center, College of Food Science and Technology, Northwest University, Xi’an, 710069, China

    Wenqi Huang, Tong Zhao, Linhua Nan, Jing Sun, Qian Liu, Linjuan Huang, Guiping Gong & Zhongfu Wang

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  1. Tingting Liu
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Liu, T., Huang, W., Zhao, T. et al. Comparative analysis of the physicochemical properties and biological activities of Ziziphus Jujuba cv. Goutouzao polysaccharides obtained by fractional precipitation. Food Measure 17, 1046–1057 (2023). https://doi.org/10.1007/s11694-022-01681-y

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