Skip to main content
Log in

Physicochemical and phytochemical properties of Tunisian carob molasses

  • Original Paper
  • Published:
Journal of Food Measurement and Characterization Aims and scope Submit manuscript

Abstract

Carob molasses is widely consumed in many Mediterranean countries, including Tunisia where it is known as ‘Rub El Kharroub’. The main objective of the present study was to evaluate the physicochemical properties and biological activities of both commercial and homemade Tunisian carob molasses. The physicochemical characterization revealed that the main parameters (color and HMF concentration) were related to non-enzymatic browning reactions occurring during juice concentration. The phytochemical analysis proved that the presence of bioactive compounds (volatile compounds, phenolic substances and products of non-enzymatic browning reactions) in carob molasses samples justify their biological effects (antioxidant and antibacterial activities). Accordingly, such characteristics may qualify Tunisian carob molasses (both homemade and commercial) as nutritious and healthy food that could be directly consumed or used a functional ingredient in food and pharmaceutical industry.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. K. Dhaouadi, M. Belkhir, I. Akinocho, F. Raboudi, D. Pamies, E. Barrajón, C. Estevan, S. Fattouch, Sucrose supplementation during traditional carob syrup processing affected its chemical characteristics and biological activities. Food Sci. Technol. 57, 1–8 (2014)

    CAS  Google Scholar 

  2. L. Tounsi, S. Karra, H. Kechaou, N. Kechaou, Processing, physico-chemical and functional properties of carob molasses and powders. J. Food Meas. Charact. 11, 1440–1448 (2017)

    Google Scholar 

  3. O.B. Karaca, I.B. Saydam, M. Güven, Physicochemical, mineral and sensory properties of set-type yoghurts produced by addition of grape, mulberry and carob molasses (Pekmez) at different ratios. Int. J. Dairy Technol. 65, 111–117 (2012)

    CAS  Google Scholar 

  4. F. Abbès, W. Kchaou, C. Blecker, M. Ongena, G. Lognay, H. Attia, S. Besbes, Effect of processing conditions on phenolic compounds and antioxidant properties of date syrup. Ind. Crops Prod. 44, 634–642 (2013)

    Google Scholar 

  5. K. Dhaouadi, F. Raboudi, C. Estevan, E. Barrajon, E. Vilanova, M. Hamdaoui, S. Fattouch, Cell viability effects and antioxidant and antimicrobial activities of Tunisian date syrup (Rub El Tamer) polyphenolic extracts. J. Agric. Food Chem. 59, 402–406 (2011)

    CAS  PubMed  Google Scholar 

  6. K. Dhaouadi, F. Raboudi, L. Funez-Gomez, D. Pamies, C. Estevan, M. Hamdaoui, S. Fattouch, Polyphenolic extract of barbary-fig (Opuntia ficus-indica) syrup: RP-HPLC-ESI-MS analysis and determination of antioxidant, antimicrobial and cancer-cells cytotoxic potentials. Food Anal. Methods 6, 45–53 (2013)

    Google Scholar 

  7. B.S. Wang, L.W. Chang, Z.C. Kang, H.L. Chu, H.M. Tai, M.H. Huang, Inhibitory effects of molasses on mutation and nitric oxide production. Food Chem. 126, 1102–1107 (2011)

    CAS  Google Scholar 

  8. M. Sengül, M.F. Ertugay, M. Sengül, Y. Yüksel, Rheological characteristics of carob Pekmez. Int. J. Food Prop. 10, 39–46 (2007)

    Google Scholar 

  9. H. Vaikousi, K. Koutsoumanis, C.G. Biliaderis, Kinetic modelling of non-enzymatic browning of apple juice concentrates differing in water activity under isothermal and dynamic heating conditions. Food Chem. 107, 785–796 (2008)

    CAS  Google Scholar 

  10. S. Benjakul, W. Visessanguan, V. Phongkanpai, M. Tanaka, Antioxidative activity of caramelisation products and their preventive effect on lipid oxidation in fish mince. Food Chem. 90, 231–239 (2005)

    CAS  Google Scholar 

  11. J.A. Rufián-Henares, F.J. Morales, Functional properties of melanoidins: In vitro antioxidant, antimicrobial and antihypertensive activities. Food Res. Int. 40, 995–1002 (2007)

    Google Scholar 

  12. L. Tounsi, N. Kechaou, Le caroubier (Ceratonia siliqua L.) et ses fruits: descriptions, intérêts et applications (Éditions Universitaires Européennes, Sarrebruck, 2017)

    Google Scholar 

  13. M.M. Özcan, D. Arslan, H. Gökçalik, Some compositional properties and mineral contents of carob (Ceratonia siliqua) fruit, flour and syrup. Int. J. Food Sci. Nutr. 58, 652–658 (2007)

    PubMed  Google Scholar 

  14. A. Şimşek, N. Artik, Studies of composition of concentrates from different fruit. GIDA 27, 459–467 (2002)

    Google Scholar 

  15. N. Tetik, İ. Turhan, M. Karhan, H.R. Öziyci, Characterization of, and 5-hydroxymethylfurfural concentration in carob Pekmez. GIDA 35, 417–422 (2010)

    Google Scholar 

  16. N. Tetik, I. Turhan, H.R. Oziyci, M. Karhan, Determination of d-pinitol in carob syrup. Int. J. Food Sci. Nutr. 62, 572–576 (2011)

    CAS  PubMed  Google Scholar 

  17. O.S. Toker, M. Dogan, N.B. Ersöz, M.T. Yilmaz, Optimization of the content of 5-hydroxymethylfurfural (HMF) formed in some molasses types: HPLC-DAD analysis to determine effect of different storage time and temperature levels. Ind. Crop. Prod. 50, 137–144 (2013)

    CAS  Google Scholar 

  18. CIE, Colorimetry, Supplement No. 2, Publication No. 15. (Commision International de l’Eclairage, Paris, France, 1986)

  19. AFNOR, Jus de fruits et de légumes - Détermination de l’acidité titrable. NF EN 12147. (Association Française de Normalisation, Paris, France, 1997)

  20. N. Turkmen, F. Sari, E.S. Poyrazoglu, Y.S. Velioglu, Effects of prolonged heating on antioxidant activity and colour of honey. Food Chem. 95, 653–657 (2006)

    CAS  Google Scholar 

  21. A.E. Cohen, Y. Birk, C. Mannheim, I. Saguy, A rapid method to monitor quality of apple juice during thermal processing. Food Sci. Technol. 31, 612–616 (1998)

    Google Scholar 

  22. A. Orphanides, V. Goulas, M. Chrysostomou, V. Gekas, Recovery of essential oils from carobs through various extraction methods, in Recent Advances in Environment, Energy Systems and Naval Science (2011), pp. 219–224

  23. M. Dubois, K.A. Gilles, J.K. Hamilton, P.A. Rebers, F. Smith, Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350–356 (1956)

    CAS  Google Scholar 

  24. G.L. Miller, Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31, 426–428 (1959)

    CAS  Google Scholar 

  25. AOAC, Official Methods of Analysis, 17th edn. (Association of Official Analytical Chemists, Washington, DC, 2000)

    Google Scholar 

  26. L.Y. Chew, K.N. Prasad, I. Amin, A. Azrina, C.Y. Lau, Nutritional composition and antioxidant properties of Canarium odontophyllum Miq. (dabai) fruits. J. Food Compos. Anal. 24, 670–677 (2011)

    CAS  Google Scholar 

  27. AFNOR, Jus de fruits et de légumes - Dosage des minéraux par spectrométrie d’absorption atomique. NF V76-117. (Association Française de Normalisation, Paris, France, 1994)

  28. R.E. Kitson, M.G. Mellon, Colorimetric determination of phosphorus as molybdivanadophosphoric acid. Ind. Eng. Chem. Anal. Ed. 16, 379–383 (1944)

    CAS  Google Scholar 

  29. V.L. Singleton, J.A. Rossi, Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 16, 144–158 (1965)

    CAS  Google Scholar 

  30. R. Avallone, M. Plessi, M. Baraldi, A. Monzani, Determination of chemical composition of carob (Ceratonia siliqua): protein, fat, carbohydrates, and tannins. J. Food Compos. Anal. 10, 166–172 (1997)

    CAS  Google Scholar 

  31. P. Prieto, M. Pineda, M. Aguilar, Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal. Biochem. 269, 337–341 (1999)

    CAS  PubMed  Google Scholar 

  32. P. Bersuder, M. Hole, G. Smith, Antioxidants from a heated histidine-glucose model system. I: Investigation of the antioxidant role of histidine and isolation of antioxidants by high-performance liquid chromatography. J. Am. Oil Chem. Soc. 75, 181–187 (1998)

    CAS  Google Scholar 

  33. A. Yildirim, A. Mavi, A.A. Kara, Determination of Antioxidant and Antimicrobial activities of Rumex crispus L. Extracts. J. Agric. Food Chem. 49, 4083–4089 (2001)

    CAS  PubMed  Google Scholar 

  34. D. A. Van den Berghe, A. J. Vlietinck, Screening methods for antibacterial and antiviral agents from higher plants. in Methods in Plant Biochemistry. (Academic Press, London, 1991), pp. 47–69

  35. A.S. Karaman, A. Kayacier, Effect of temperature on rheological characteristics of molasses: modeling of apparent viscosity using adaptive neuro-fuzzy inference system (ANFIS). Food Sci. Technol. 44, 1717–1725 (2011)

    CAS  Google Scholar 

  36. A. Guilherme, T.L. Honorato, A.S. Dornelles, G.A.S. Pinto, E.S. Brito, S. Rodrigues, Quality evaluation of mesquite (Prosopis juliflora) pods and cashew (Anacardium occidentale) apple syrups. J. Food Process Eng. 32, 606–622 (2009)

    Google Scholar 

  37. IEC, Multimedia systems and equipment: colour measurement and management, International Standard 61966-2-1. (International Electrotechnical Commission, Geneva, Switzerland, 1999)

  38. M. Akbulut, H. Coklar, G. Ozen, Rheological characteristics of Juniperus drupacea fruit juice (Pekmez) concentrated by boiling. Food Sci. Technol. Int. 14, 321–328 (2008)

    CAS  Google Scholar 

  39. Codex Alimentarius, List of codex specifications for food additives, CAC/MISC 6-2013 (2013)

  40. A. Dehpour, B. Babakhani, S. Khazaei, M. Asadi, Chemical composition of essential oil and antibacterial activity of extracts from flower of Allium atroviolaceum. J. Med. Plants Res. 5, 3667–3672 (2011)

    CAS  Google Scholar 

  41. A. Djilani, A. Dicko, The therapeutic benefits of essential oils, in Nutrition, Well-Being and Health. (InTech, New York, 2012), pp. 155–178

    Google Scholar 

  42. M.M. Raj, H.V. Patel, L.M. Raj, N.K. Patel, Synthesis, characterization and in-vitro antimicrobial evaluation of some novel isoxazoline derivatives. Int. J. Res. Pharm. Chem. 3, 612–618 (2013)

    CAS  Google Scholar 

  43. A. Ben Hsouna, M. Trigui, R. Ben Mansour, R.M. Jarraya, M. Damak, S. Jaoua, Chemical composition, cytotoxicity effect and antimicrobial activity of Ceratonia siliqua essential oil with preservative effects against Listeria inoculated in minced beef meat. Int. J. Food Microbiol. 148, 66–72 (2011)

    CAS  Google Scholar 

  44. M.J. Cantalejo, Effects of roasting temperature on the aroma components of carob (Ceratonia siliqua L.). J. Agric. Food Chem. 45, 1345–1350 (1997)

    CAS  Google Scholar 

  45. M.A. Farag, D.M. El-Kersh, Volatiles profiling in Ceratonia siliqua (Carob bean) from Egypt and in response to roasting as analyzed via solid-phase microextraction coupled to chemometrics. J. Adv. Res. 8, 379–385 (2017)

    CAS  PubMed  PubMed Central  Google Scholar 

  46. G. Macleod, M. Forcen, Analysis of volatile components derived from the carob. Phytochemistry 31, 3113–3119 (1992)

    CAS  Google Scholar 

  47. M.E. Wakefield, G.P. Bryning, L.E. Collins, J. Chambers, Identification of attractive components of carob volatiles for the foreign grain beetle, Ahasverus advena (Waltl) (Coleoptera: Cucujidae). J. Stored Prod. Res. 41, 239–253 (2005)

    CAS  Google Scholar 

  48. ‬M. Papagiannopoulos, H. R. Wollseifen, A. Mellenthin, B. Haber, R. Galensa, Identification and quantification of polyphenols in carob fruits (Ceratonia siliqua) and derived products by HPLC-UV-ESI/MS. J. Agric. Food Chem. 52, 3784–3791 (2004)

  49. F. Abbès, M.A. Bouaziz, C. Blecker, M. Masmoudi, H. Attia, S. Besbes, Date syrup: effect of hydrolytic enzymes (pectinase/cellulase) on physico-chemical characteristics, sensory and functional properties. Food Sci. Technol. 44, 1827–1834 (2011)

    Google Scholar 

  50. E.I. Oikeh, E.S. Omoregie, F.E. Oviasogie, K. Oriakhi, Phytochemical, antimicrobial, and antioxidant activities of different citrus juice concentrates. Food Sci. Nutr. 4, 103–109 (2016)

    CAS  PubMed  Google Scholar 

  51. S.L. Chen, D.J. Yang, H.Y. Chen, S.C. Liu, Effect of hot acidic fructose solution on caramelisation intermediates including colour, hydroxymethylfurfural and antioxidative activity changes. Food Chem. 114, 582–588 (2009)

    CAS  Google Scholar 

  52. G. Hwang, H.Y. Kim, K.S. Woo, J. Lee, H.S. Jeong, Biological activities of Maillard reaction products (MRPs) in a sugar-amino acid model system. Food Chem. 126, 221–227 (2011)

    CAS  Google Scholar 

  53. M.I. Halpin-Dohnalek, E.H. Marth, Staphylococcus aureus: production of extracellular compounds and behavior in foods—a review. J. Food Prot. 52, 267–282 (1989)

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors would like to thank the industry “Confiserie Triki-le Moulin” (Sfax, Tunisia) for the financial support and analytical assistance. The authors are also grateful to all the Tunisian families (Bekalta, Monastir) for providing kindly carob molasses samples and explaining their manufacturing process.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Leila Tounsi.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tounsi, L., Ghazala, I. & Kechaou, N. Physicochemical and phytochemical properties of Tunisian carob molasses. Food Measure 14, 20–30 (2020). https://doi.org/10.1007/s11694-019-00263-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11694-019-00263-9

Keywords

Navigation