Skip to main content
SpringerLink
Log in

Comprehensive determination of nine polyphenols in Polygoni Avicularis Herba with a new HPLC–DAD method and their correlation with the antioxidant activities

  • Original Paper
  • Published:
Journal of Food Measurement and Characterization Aims and scope Submit manuscript

Abstract

A simple high performance liquid chromatography (HPLC) method was established for identification and quantification of nine polyphenols (gallic acid, chlorogenic acid, caffeic acid, myricetrin, hyperoside, luteoloside, quercitrin-3-rhamnoside, quercetin and kaempferol) in Polygoni Avicularis Herba. The analysis was performed on a Pinnacle II C18 column (250 × 4.6 mm, 5.0 µm) with a gradient elution of an aqueous mobile phase (containing 0.3% acetic acid) modified by acetonitrile. Diode-array detector (DAD) was used to detect the analytes at four wavelengths (270, 326, 355, and 370 nm). The established method was validated with good linearity, precision, accuracy and reproducibility to determine the polyphenols in Polygoni Avicularis Herba. The applicability of this analytical method was confirmed by successful analysis of the herb samples. The results indicated that the contents of these polyphenols varied with different locations of the samples. The anti-oxidation activities of Polygoni Avicularis Herba were further evaluated based on DPPH, ABTS and superoxide anion free radical scavenging assay. The herb exhibited obvious radical scavenging capacity, and gallic acid, chlorogenic acid, myricetrin and hyperoside played important roles in this activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Y.Y. Sung, T. Yoon, W.K. Yang, S.J. Kim, D.S. Kim, H.K. Kim, The antiobesity effect of Polygonum aviculare L. ethanol extract in high-fat diet-induced obese mice. Evid. Based. Complement. Altern. Med. 2013, 626397 (2013)

    Article  Google Scholar 

  2. Eurpean Pharmacopoeia. (European Directorate for the Quality of Medicines & HealthCare, 2017), https://www.edqm.Eu/en/European-pharmacopoeia-9th-editin1563.html. Accessed 15 June 2017

  3. H.M.H. Salama, N. Marraiki, Antimicrobial activity and phytochemical analysis of Polygonum aviculare L. (Polygonaceae), naturally growing in Egypt. Saudi. J. Biol. Sci. 17, 57–63 (2010)

    Article  CAS  PubMed  Google Scholar 

  4. H. Tunon, C.L. Bohlin, Evaluation of anti-inflammatory activity of some Swedish medicinal plants. Inhibition of prostaglandin biosynthesis and PAF-induced exocytosis. J. Ethnopharmacol. 48, 61–76 (1995)

    Article  CAS  PubMed  Google Scholar 

  5. S.H. Park, Y.Y. Sung, K.J. Nho, H.K. Kim, Anti-atherosclerotic effects of Polygonum aviculare L. ethanol extract in ApoE knock-out mice fed a Western diet mediated via the MAPK pathway. J. Ethnopharmacol. 151, 1109–1115 (2014)

    Article  Google Scholar 

  6. J.X. Nan, E.J. Park, H.J. Kim, G. Ko, D.H. Sohn, Antifibrotic effects of the methanol extract of Polygonum aviculare in fibrotic rats induced by bile duct ligation and scission. Biol. Pharm. Bull. 23, 240–243 (2000)

    Article  CAS  PubMed  Google Scholar 

  7. R.M. Habibi, R.A. Mohammadi, A. Delazar, R. Halabian, R.J. Soleimani, A. Mehdipour, M. Bagheri, A. Jahanian-Najafabadi, Effect of Polygonum aviculare herbal extract on proliferation and apoptotic gene expression of MCF-7. DARU J. Pharm. Sci. 19, 326–331 (2011)

    Google Scholar 

  8. C.Y. Hsu, Antioxidant activity of extract from Polygonum aviculare L. Biol. Res. 39, 281–288 (2006)

    Article  PubMed  Google Scholar 

  9. C. Nichita, C. Bazdoaca, M. Giurginca, A. Meghea, Chemical-structural characteristics and the anti-oxidative capacity of some plant extracts obtained by classical and modern technique. Rev. Chim. 56, 495–498 (2005)

    CAS  Google Scholar 

  10. N.A. Yunuskhodzhaeva, K.A. Eshbakova, V.N. Abdullabekova, Flavonoid composition of the herb Polygonum aviculare. Chem. Nat. Compd. 46, 803–804 (2010)

    Article  CAS  Google Scholar 

  11. S. Granica, M.E. Czerwinska, B. Zyzynska-Granica, A.K. Kiss, New acetylated flavonol glucuronides isolated from Polygoni avicularis herba and their influence on some neutrophiles’ functions. Planta Med. 79, 1214–1214 (2013)

    Article  Google Scholar 

  12. A. Nugroho, E.J. Kim, J.S. Choi, H.J. Park, Simultaneous quantification and peroxynitrite-scavenging activities of flavonoids in Polygonum aviculare L. herb. J. Pharm. Biomed. 89, 93–98 (2014)

    Article  CAS  Google Scholar 

  13. G.G. Nikolaeva, M.V. Lavrent’eva, I.G. Nikolaeva, Phenolic compounds from several Polygonum species. Chem. Nat. Compd. 45, 735–736 (2009)

    Article  CAS  Google Scholar 

  14. U. Sawicka, W. Cisowski, G. Matysik, A. Kowalczyk, HPTLC of phenolic acids in Bistortae rhizoma, Polygoni avicularis herba, Rhei radix with densitometric determination. JPC 15, 442–448 (2002)

    CAS  Google Scholar 

  15. J.B. Xie, Y.Q. Zhang, D.Q. Kong, M. Rexit, Rapid identification and determination of 11 polyphenols in Herba lycopi by HPLC-MS/MS with multiple reactions monitoring mode (MRM). Food Compos. Anal. 24, 1069–1072 (2011)

    Article  CAS  Google Scholar 

  16. S. Granica, Phytochemical investigations of Polygoni avicularis herba. Quantification of phenolic compounds with UHPLC-CAD method. Planta Med. 81, 1540–1540 (2015)

    Google Scholar 

  17. S. Granica, Quantitative and qualitative investigations of pharmacopoeial plant material polygoni avicularis herba by UHPLC-CAD and UHPLC-ESI-MS methods. Phytochem. Anal. 26, 374–382 (2015)

    Article  CAS  PubMed  Google Scholar 

  18. S. Granica, J.P. Piwowarski, M. Poplawska, M. Jakubowska, J. Borzym, A.K. Kiss, Novel insight into qualitative standardization of Polygoni avicularis herba (Ph. Eur.). J. Pharm. Biomed. 72, 216–222 (2013)

    Article  CAS  Google Scholar 

  19. J.B. Xie, W.Q. Wang, Y.Q. Zhang, Y. Bai, Q. Yang, Simultaneous analysis of glycyrrhizin, paeoniflorin, quercetin, ferulic acid, liquiritin, formononetin, benzoic acid and isoliquiritigenin in the Chinese proprietary medicine Xiao Yao Wan by HPLC. J. Pharm. Biomed. 45, 450–455 (2007)

    Article  CAS  Google Scholar 

  20. C. Sarikurkcu, B. Tepb, D. Daferera, M. Polissiou, M. Harmandar, Studies on the antioxidation activity of the essential oil and methanol extract of Marrubium globosum subsp. Globosum (Lamiaceae) by three different chemical assays. Bioresour. Technol. 99, 4239–4246 (2008)

    Article  CAS  PubMed  Google Scholar 

  21. W. Chen, Y. Shen, H.M. Su, X.D. Zheng, Hispidin derived from Phellinus linteus affords protection against acrylamide-induced oxidative stress in Caco-2 cells. Chemico 219, 83–89 (2014)

    CAS  Google Scholar 

  22. D.R. Pompeu, E.M. Silva, H. Rogez, Optimisation of the solvent extraction of phenolic antioxidants from fruits of euterpeoleracea using response surface methodology. Bioresour. Technol. 100, 6076–6082 (2009)

    Article  CAS  PubMed  Google Scholar 

  23. J.B. Xie, L.T. Sang, Y.Q. Zhang, L.L. Fang, Y.X. Li, Determination of stachydrine and leonurine in Herba Leonuri and its succedaneum-Herba Lagopsis with a sensitive HPLC-MS/MS method. J. Liq. Chromatogr. Relat. Technol. 38, 810–815 (2015)

    Article  CAS  Google Scholar 

  24. Chinese Pharmacopoeia Commission, Pharmacopoeia of People’s Republic of China (part 1). (Pharmacopoeia Commission of People’s Republic of China (China Medical Science and Technology Press, Beijing, 2015), pp. 334–335

    Google Scholar 

  25. Q. Fu, S. Liu, H. Wang, S.Z. Chen, Simultaneous determination of eight flavonoids in Polygonum aviculare L. by RP-HPLC-UV. J. Pharm. Sci. 23, 170–176 (2014)

    CAS  Google Scholar 

  26. S. Miraghaei, B. Mohammadi, A. Babaei, S. Keshavarz, Development and validation of a new HPLC-DAD method for quantification of sofosbuvir in human serum and its comparison with LC–MS/MS technique: application to a bioequivalence study. J. Chromatogr. B 1063, 118–122 (2017)

    Article  CAS  Google Scholar 

  27. M. Zhu, J.A. Duan, Y.P. Tang, J.M. Guo, E.X. Shang, Z.H. Zhu, Identification of chemical constituents in SiWu decoction by UHPLC-DAD-TOF/MS. Acta Chromatogr. 26, 517–537 (2014)

    Article  CAS  Google Scholar 

  28. N. Nenadis, L.F. Wang, M. Tsimidou, H.Y. Zhang, Estimation of scavenging activity of phenolic compounds using the ABTS·+ assay. J. Agric. Food Chem. 52, 4669–4674 (2004)

    Article  CAS  PubMed  Google Scholar 

  29. H. Ozbek, Z. Guvenalp, T. Ozek, H.G. Sevindik, H. Yuca, K.O. Yerdelen, Chemical composition, antioxidant and anticholinesterase activities of the essential oil of Origanum rotundifolium Boiss. from Turkey. Rec. Nat. Prod. 11, 484–490 (2017)

    Article  Google Scholar 

  30. L.C. Zhu, J.Q. Chen, J. Tan, X. Liu, B.C. Wang, Flavonoids from Agrimonia pilosa Ledeb: free radical scavenging and DNA oxidative damage protection activities and analysis of bioactivity-structure relationship based on molecular and electronic structures. Molecules. 2, 195 (2017)

    Article  CAS  Google Scholar 

  31. Y.L. Xiong, Inhibition of hazardous compound formation in muscle foods by antioxidative phytophenols. Ann. N.Y. Acad. Sci. 1398, 37–46 (2017)

    Article  CAS  PubMed  Google Scholar 

  32. S.K. Chung, Y.C. Kim, Y. Takaya, K. Terashima, M. Niwa, Novel flavonol glycoside, 7-O-methyl mearnsitrin, from Sageretia theezans and its antioxidant effect. J. Agr. Food Chem. 52, 4664–4668 (2004)

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by “131” innovative talents training project in Tianjin, and Tianjin Distinguished Professor project (2013-Wen).

Author information

Authors and Affiliations

  1. College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, 300134, China

    Lijuan Jiao, Chenpu Yan, Kunsheng Zhang, Junbo Xie, Yanqing Zhang & Zhiyou Wen

  2. Tianjin Key Laboratory of Food Biotechnology, Tianjin, 300134, China

    Kunsheng Zhang, Junbo Xie & Yanqing Zhang

  3. Department of Food Science and Human Nutrition, Iowa State University, Ames, IA, 50010, USA

    Zhiyou Wen

Authors
  1. Lijuan Jiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chenpu Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kunsheng Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Junbo Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yanqing Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Zhiyou Wen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Yanqing Zhang or Zhiyou Wen.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jiao, L., Yan, C., Zhang, K. et al. Comprehensive determination of nine polyphenols in Polygoni Avicularis Herba with a new HPLC–DAD method and their correlation with the antioxidant activities. Food Measure 12, 1593–1600 (2018). https://doi.org/10.1007/s11694-018-9774-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11694-018-9774-8

Keywords

Search

Navigation