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Transcriptome-wide identification and expression profiling of Pinus massoniana MYB transcription factors responding to phosphorus deficiency

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Abstract

Myeloblastosis (MYB) proteins constitute one of the largest transcription factor (TF) families in plants and play crucial roles in regulating plant physiological and biochemical processes, including adaptation to diverse abiotic stresses. These TF families contain highly conserved MYB repeats (1R, R2R3, 3R and 4R) at the N-terminus. Roles for MYB TFs have been reported in response to such stresses as dehydration, salt, cold, and drought. The characterization of Masson pine (Pinus massoniana) MYB TFs are reported, including the analysis of MYB TFs expression in seedlings under controlled conditions and two different phosphate (Pi) deficient treatments. By searching for conserved MYB motifs in full transcriptomic RNA sequencing data for P. massoniana, 59 sequences were identified as MYB TFs. Conserved domain structures and comparative functional and phylogenetic relationships of these MYB TFs with those in Arabidopsis were assessed using various bioinformatics tools. Based on microarray analysis, P. massoniana MYB genes exhibited different expression patterns under the two Pi deficiency conditions. Genes encoding MYB TFs that showed increased expression under critical Pi deficiency were identified, and some MYBs were differentially expressed only under conditions of severe Pi starvation. These results are useful for the functional characterization of MYB TFs that may be involved in the response to Pi deficiency and play divergent roles in plants.

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Authors and Affiliations

  1. Institute for Forest Resources and Environment of Guizhou, Guizhou University, Guiyang, 550025, China

    Fuhua Fan & Guijie Ding

  2. College of Forestry, Guizhou University, Guiyang, 550025, China

    Fuhua Fan, Qingzhu Wang & Guijie Ding

  3. Institute of Agro-Bioengineering, Guizhou University, Guiyang, 550025, China

    Qingzhu Wang & Xiaopeng Wen

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  1. Fuhua Fan
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  2. Qingzhu Wang
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  4. Guijie Ding
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Correspondence to Fuhua Fan.

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Project funding: This study was funded by the Natural Science Foundation of Guizhou Province, P. R. China (20161051), the National Natural Science Foundation of China (31660185) and the Doctoral Fund Project of Guizhou University, P. R. China (201464).

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Corresponding editor: Yu Lei.

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Supplementary Fig. S1 The conserved motifs in the MYB proteins of P. massoniana (TIFF 350 kb)

11676_2019_911_MOESM5_ESM.tif

Supplementary Fig. S2 Conserved amino acid residues are present among the 59 proteins of the P. massoniana MYBs. The amino acid sequences are aligned, and gaps (dots) have been introduced to maximize the alignment (TIFF 3687 kb)

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Fan, F., Wang, Q., Wen, X. et al. Transcriptome-wide identification and expression profiling of Pinus massoniana MYB transcription factors responding to phosphorus deficiency. J. For. Res. 31, 909–919 (2020). https://doi.org/10.1007/s11676-019-00911-2

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