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Characterization of a collection of Chinese fir elite genotypes using sequence-related amplified polymorphism markers

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Abstract

Chinese fir [Cunninghamia lanceolata (Lamb.) Hook] is a major timber tree species in China. In the present study, sequence-related amplified polymorphism (SRAP) markers were used to evaluate the polymorphisms, diversity, and relationships in a collection of Chinese fir elite genotypes (n = 103) from the Lechang provenance, an endangered provenance. Thirty-five SRAP primer combinations produced 620 bands among the tested 103 Lechang provenance genotypes and 17 non-Lechang provenance genotypes; 577 (93.1 %) of these were polymorphic, yielding an average of 16.5 polymorphic bands per primer combination. Of the SRAP primer combinations (n = 35), 14 ones identified ≥70.0 % of the 120 genotypes. The Me12–Em19 combination had the strongest discriminatory ability with 93.3 % of the genotypes identified. An UPGMA dendrogram further showed that most of the Lechang provenance genotypes (87.4 %) grouped together (Cluster A). The Lechang genotypes had a considerable amount of genetic variation with genetic similarity from 0.40 to 0.81. The current work may facilitate the management of the endangered Lechang Chinese fir provenance.

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Acknowledgments

We thank LetPub (www.letpub.com) for linguistic assistance during the preparation of this manuscript.

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Authors and Affiliations

  1. National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Hongjing Duan & Yun Li

  2. Guangdong Provincial Key Laboratory of Bio-control for the Forest Disease and Pest, Guangdong Academy of Forestry, Guangzhou, 510520, People’s Republic of China

    Hongjing Duan, Dehuo Hu & Huiquan Zheng

Authors
  1. Hongjing Duan
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  2. Dehuo Hu
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  3. Yun Li
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  4. Huiquan Zheng
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Corresponding author

Correspondence to Huiquan Zheng.

Additional information

Project funding: This work was supported by the National Natural Sciences Foundation of China (No. 31200506), the Special Fund for Forest Scientific Research in the Public Welfare (No. 201404127), the Special Plan for the Cultivation of High-level Talents of Guangdong (No. 2014TQ01N140), and the National ‘863’ Project of the 12th Five-Year Plan in China (2011AA100203).

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Corresponding editor: Hu Yanbo

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Duan, H., Hu, D., Li, Y. et al. Characterization of a collection of Chinese fir elite genotypes using sequence-related amplified polymorphism markers. J. For. Res. 27, 1105–1110 (2016). https://doi.org/10.1007/s11676-016-0233-2

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  • DOI: https://doi.org/10.1007/s11676-016-0233-2

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