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Genetic diversity and population structure of Butea monosperma (Lam.) Taub.- a potential medicinal legume tree

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Abstract

Three molecular marker systems, Random Amplified Polymorphic DNA (RAPD), Inter-Simple Sequence Repeats (ISSR) and Sequence-Related Amplified Polymorphism (SRAP) were employed to investigate the genetic structure and diversity among the 14 natural populations of Butea monosperma collected from different geographical regions of India. Detected by 17 RAPD, 15 ISSR and 11 SRAP primer combinations, the proportions of polymorphic bands were 84.2 %, 77.2 % and 91.9 %, respectively, and the mean Nei’s genetic distances among the populations were 0.13, 0.10 and 0.13, respectively. Partitioning of genetic variability by Analysis of molecular variance (AMOVA) revealed that the high genetic diversity was distributed within the populations. AMOVA also revealed that the coefficient of gene differentiation among populations based on FST was very high irrespective of markers used. The overall gene flow among populations (Nm) was very low. Cophenetic correlation coefficients of Nei’s distance values and clustering pattern by Mental test were statistically significant for all three marker systems used but poor fit for ISSR data than for RAPD, SRAP and combined data set of all three markers. For all markers, a high similarity in dendrogram topologies was obtained, although some differences were observed with ISSR. The dendrogram obtained by RAPD, SRAP and combined data set of all three markers reflect relationship of most of the populations according to their geographic distribution.

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Acknowledgements

Authors are highly thankful and gratefully acknowledge Dr. KV Bhat, Principal Scientist, National Bureau of Plant Genetic Resources (NBPGR) New Delhi for helping in data analysis and to Department of Biotechnology, New Delhi (India) for financial support to carry out the present study.

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  1. Department of Botany, University of Delhi, Delhi, 110007, India

    Amit Vashishtha, Tabassum Jehan & Suman Lakhanpaul

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  1. Amit Vashishtha
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  2. Tabassum Jehan
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  3. Suman Lakhanpaul
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Correspondence to Suman Lakhanpaul.

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Vashishtha, A., Jehan, T. & Lakhanpaul, S. Genetic diversity and population structure of Butea monosperma (Lam.) Taub.- a potential medicinal legume tree. Physiol Mol Biol Plants 19, 389–397 (2013). https://doi.org/10.1007/s12298-013-0170-x

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