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High-frequency direct shoot induction from leaf explants of Pogostemon quadrifolius (Benth.) F. Muell.: an ethnomedicinal herb

  • Plant Tissue Culture
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Abstract

Pogostemon quadrifolius is an important lateritic plant with high ethnomedicinal and ecological value. Here, we describe an efficient and rapid in vitro propagation protocol via direct regeneration from leaf explants of P. quadrifolius. In vitro leaves obtained from nodal segment culture were inoculated on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzylaminopurine (BAP; 0.44 to 6.66 μM), thidiazuron (TDZ; 0.454 to 6.81 μM), and kinetin (KN; 0.465 to 6.97 μM) alone and in combination with α-naphthaleneacetic acid (NAA; 0.537 to 10.74 μM). The highest frequency of response (96.5%) and shoot induction (79.1 shoots per explant) was obtained on MS medium with 2.22 μM BAP and 8.055 μM NAA. The optimum root induction (98.1%) and root number (16.24) were observed in a half-strength MS medium containing 2.46 μM indole-3-butyric acid (IBA). The plantlets with well-developed roots were acclimatized in small plastic cups containing sterilized vermiculite and coconut husk mixture (1:1) and later successfully shifted to the field. To our knowledge, this is the first report of in vitro organogenesis in P. quadrifolius. This optimized protocol is cost-effective and highly efficient for the rapid mass propagation of P. quadrifolius and is a prerequisite to meet pharmaceutical and ecological needs.

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Funding

Deepa A. V. thankfully acknowledges the financial support from CSIR, Govt. of India, in the form of JRF (award no. 09/1108(0011)/2016-EMR-I).

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  1. Department of Plant Science, Central University of Kerala, Tejaswini Hills, Periye, Kasaragod, Kerala, PIN-671314, India

    A. V. Deepa & T. Dennis Thomas

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  1. A. V. Deepa
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Correspondence to T. Dennis Thomas.

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Deepa, A.V., Thomas, T.D. High-frequency direct shoot induction from leaf explants of Pogostemon quadrifolius (Benth.) F. Muell.: an ethnomedicinal herb. In Vitro Cell.Dev.Biol.-Plant 58, 321–329 (2022). https://doi.org/10.1007/s11627-022-10265-w

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