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Buffalo (Bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro

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Abstract

Recent studies suggested that placentae amniotic membrane is a valuable source of stem cells in human as well as in livestock species. Advantages of amnion over other sources of stem cells included abundant availability, ethically non-objectionable and non-invasive source. The aim of the present study was the isolation, culture and characterization of amniotic-membrane-derived mesenchymal stem cells from term placentae collected postpartum in buffalo. We have observed that both presumptive epithelial-like and fibroblast-like cells were cultured and maintained from term amnion. These cells were shown the positive expression of pluripotency markers (OCT-4, SOX-2, NANOG, TERT), mesenchymal stem cell markers (CD29, CD44, CD105) and negative for haematopoietic marker (CD34) genes at different passages. In addition, these cells were also positive for alkaline phosphatase staining. Stem-ness potential of any stem cells is determined by their potential to differentiate into specific lineages of cell type. In the present study, we have successfully differentiated the amniotic-membrane-derived cells into adipogenic, chondrogenic and osteogenic lineages of cells in vitro. In conclusion, the results of this study demonstrate that amniotic-membrane-derived cells expressed pluripotent and mesenchymal stem cells markers and have propensity to differentiate into cells of mesenchymal lineage cell type upon directed differentiation in vitro.

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Acknowledgments

The present work was funded by institutional research project grant, ICAR-Central Institute for Research on Buffaloes to Dr. PS Yadav. KG is a recipient of University Research Scholarship from Department of Biotechnology, Chaudhary Devi Lal University, Sirsa-India.

Conflict of interest

The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.

Author contributions

Conceived and designed the experiments: PSY, RK, JS and SKG. Performed the experiments: KG, RK and JS. Contributed reagents/materials/analysis tools: PSY, DK, BRG and SPY. Wrote the paper: NLS, JS and KG.

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Authors and Affiliations

  1. Animal Physiology and Reproduction Division, Central Institute for Research on Buffaloes, Hisar, 125001, Haryana, India

    Kaushalya Ghosh, Dharmendra Kumar, Naresh Lalaji Selokar, S. P. Yadav & P. S. Yadav

  2. Department of Biotechnology, Chaudhary Devi Lal University, Sirsa, 125055, Haryana, India

    Kaushalya Ghosh & S. K. Gahlawat

  3. National Research Centre on Equines, Hisar, 125001, Haryana, India

    Rajesh Kumar, Jarnail Singh & B. R. Gulati

Authors
  1. Kaushalya Ghosh
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  2. Rajesh Kumar
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  3. Jarnail Singh
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  4. S. K. Gahlawat
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  5. Dharmendra Kumar
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  6. Naresh Lalaji Selokar
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  7. S. P. Yadav
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  8. B. R. Gulati
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  9. P. S. Yadav
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Corresponding author

Correspondence to P. S. Yadav.

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Editor: T. Okamoto

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Ghosh, K., Kumar, R., Singh, J. et al. Buffalo (Bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro . In Vitro Cell.Dev.Biol.-Animal 51, 915–921 (2015). https://doi.org/10.1007/s11626-015-9920-0

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  • DOI: https://doi.org/10.1007/s11626-015-9920-0

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