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Aptamer-wrapped gold nanoparticles for the colorimetric detection of omethoate

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Abstract

Organophosphorous pesticide (OP) contamination could have serious adverse effects on human health and the environment. Due to the toxicity of OPs and the threat presented by their accidental or intentional release in populated areas, the determination and monitoring of these OPs in food products and environment is of great importance. OPs are present in very small quantities and therefore, methods for their detection need to be highly sensitive and selective. Here, we aimed to develop a simple and selective aptamer-based colorimetric assay for the detection of omethoate, which is one of the commonly used OPs. The principle of the assay is that single-stranded DNA (ssDNA)-wrapped gold nanoparticles (AuNPs) are resistant to salt-induced aggregation. By employing an “artificial antibody” organophosphorous pesticide-binding aptamer (OBA) as the recognition element, aptamer-wrapped AuNPs (Au-apta) show high selectivity towards omethoate, resulting in the disconnection of aptamers from AuNPs and the aggregation of AuNPs. As there is a significant color change from the interparticle plasmon coupling during the aggregation of AuNPs, the established assay showed good linearity between 0.1 and 10 μmol/L, with a low detection limit of 0.1 μmol/L. Other OPs such as profenofos, phorate, and isocarbophos would not interfere with the detection of omethoate despite having similar structures. Thus, the colorimetric method shows potential for use in the detection of omethoate in real soil samples.

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Authors and Affiliations

  1. School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China

    Ying Wan, Yan Su & Chunhai Fan

  2. School of Environmental and Biological and Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China

    Pengjuan Wang, Ying Wan, Shengyuan Deng & Shulin Yang

  3. Chemistry Department, King Saud University, Riyadh, 1145, Saudi Arabia

    Aldalbahi Ali

  4. Laboratory of Physical Biology, Chinese Academy of Sciences, Shanghai, 201800, China

    Chunhai Fan

  5. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China

    Chunhai Fan

Authors
  1. Pengjuan Wang
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  2. Ying Wan
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  3. Aldalbahi Ali
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  4. Shengyuan Deng
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  5. Yan Su
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  6. Chunhai Fan
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  7. Shulin Yang
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Correspondence to Ying Wan or Shulin Yang.

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Wang, P., Wan, Y., Ali, A. et al. Aptamer-wrapped gold nanoparticles for the colorimetric detection of omethoate. Sci. China Chem. 59, 237–242 (2016). https://doi.org/10.1007/s11426-015-5488-5

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  • DOI: https://doi.org/10.1007/s11426-015-5488-5

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