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Monitoring benthic microflora in river bed sediments: a case study in the Anllóns River (Spain)

  • Sediments, Sec 4 • Sediment-Ecology Interactions • Research Article
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Abstract

Purpose

The objectives of this study were to investigate the abundance and composition of the superficial biofilm on the bed sediments of the Anllóns River (NW Spain), to evaluate the relationships between biochemical parameters and biological methods based on identification and counting, and to explore the relationships between biofilm growth and the properties of the sedimentary habitat, mainly the trophic state.

Materials and methods

Bed sediment samples (0–5 cm) were collected in two different seasons (winter and summer) at four sampling sites along the river course. Physicochemical properties of pore waters and sediments were determined. Biological properties included the determination of dehydrogenase activity (DHA) and phytopigment (Chl a Chl b and total carotenoids) concentrations, as well as taxonomic identification. For taxonomic identification, two sampling methods were compared: the Pasteur pipette method and a mini-corer method. Total and relative algal abundances (TA and RA, respectively) and genus richness were calculated. The relationships between the different variables were examined using Pearson correlations and principal component analysis.

Results and discussion

The main taxa belonged to Chlorophyta, Cyanophyta, Euglenophyta, and Heterokontophyta. The most abundant class was Bacillariophyceae, which represents >86 % of the total abundances in the superficial sediments. The highest total algal abundance and genus richness were observed in summer at the river mouth, where DHA and phytopigment concentrations were also the highest. The statistical analysis revealed positive correlations between TA and the biochemical parameters (DHA and phytopigments) as well as positive relationships of these three parameters with the physicochemical properties of the sediments, such as electrical conductivity, and the concentrations of fine particles, C, N, S, and total P.

Conclusions

The results of this study reveal the positive relationships between the biochemical properties (phytopigments and respiratory activity) and total algal abundances determined by taxonomic identification and counting. All of these properties presented evidence of a clear influence of the nutrients and organic matter contents of the sediments, pointing to the importance of the site conditions, particularly the trophic state, in the development of benthic microflora.

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Acknowledgments

The authors wish to thank the Spanish Ministry of Economy and Competitiveness for (MINECO-FEDER) for financial support (project ref. CGL2010-22059 and CGL2013-46003-P). Rosa Devesa-Rey gratefully acknowledges the financial support of the Ángeles Alvariño Programme of the Xunta de Galicia. Diego Martiñá Prieto wishes to acknowledge the financial support of the Spanish Ministry of Economy and Competitiveness for his FPI Fellowship (BES-2011-044514).

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Authors and Affiliations

  1. Dpto. Edafología y Química Agrícola, Facultad de Farmacia, Universidade de Santiago de Compostela, 15712, Santiago de Compostela, Spain

    Diego Martiñá Prieto, Francisco Díaz-Fierros & Maria Teresa Barral

  2. Centro Universitario de la Defensa, Escuela Naval Militar Marín, Universidade de Vigo, Plaza de España 2, 36920, Marín, Spain

    Rosa Devesa-Rey

  3. Dpto. Biología Vegetal y Ciencia del Suelo, Facultad de Ciencias, Universidade de Vigo, Campus de Ourense, As Lagoas s/n, 32004, Ourense, Spain

    Remigio Paradelo

  4. Dpto. Botánica, Facultad de Biología, Universidade de Santiago de Compostela, 15172, Santiago de Compostela, Spain

    Maria Penalta-Rodríguez

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  1. Diego Martiñá Prieto
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  2. Rosa Devesa-Rey
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Correspondence to Diego Martiñá Prieto.

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Prieto, D.M., Devesa-Rey, R., Paradelo, R. et al. Monitoring benthic microflora in river bed sediments: a case study in the Anllóns River (Spain). J Soils Sediments 16, 1825–1839 (2016). https://doi.org/10.1007/s11368-016-1395-4

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