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Measurement of Electron Transport System (ETS) activity in soil

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Abstract

Electron transport system (ETS) activity was measured in amended and nonamended soil by measuring the reduction of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT) to iodonitrotetrazolium formazan (INT-formazan), which can be easily extracted with methanol without interference from other compounds found in soil. A high correlation between ETS activity and oxygen consumption was observed. This technique allows rapid quantitative measurements of microbial ETS activity in soil.

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Authors and Affiliations

  1. Department of Biology, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada

    J. T. Trevors, C. I. Mayfield & W. E. Inniss

Authors
  1. J. T. Trevors
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  2. C. I. Mayfield
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  3. W. E. Inniss
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Trevors, J.T., Mayfield, C.I. & Inniss, W.E. Measurement of Electron Transport System (ETS) activity in soil. Microb Ecol 8, 163–168 (1982). https://doi.org/10.1007/BF02010449

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  • DOI: https://doi.org/10.1007/BF02010449

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