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Low-dose priming of gamma radiation enhanced cadmium tolerance in Chlamydomonas reinhardtii by modulating physio-biochemical pathways

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Abstract

Microalgae are natural biotic models for exploring the genotoxic effect of heavy metals, irradiation, other external stimuli and the toxicant elimination. The effective removal of heavy metals from the aquatic environment using microalgae has gained considerable attention. However, limited research was carried out on cadmum toxicity in microalgae and their use as bio-accumulants. Previous research suggested that low-dose priming with non-ionizing radiations, such as gamma radiation, increased heavy metal tolerance in plants and aquatic photosynthetic microalgae. In the present study, we have hypothesized the growth inhibitory physiochemical properties of cadmium (Cd) in Chlamydomonas reinhardtii, and analyzed the protective role of low-dose gamma radiations priming against Cd-induced growth inhibition by emphasizing mechanism of cell survival by antioxidant defence system. Experimentally, the gamma-primed C. reinhardtii exhibited higher cell survival and Cd tolerance with effective modulation of biochemical responses such as antioxidant enzymes. The current investigation revealed that low-dose priming of gamma radiation masks Cd-mediated oxidative stress and enhances cellular detoxification via intracellular antioxidant enzymes in C. reinhardtii.

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Acknowledgements

The authors are thankful to Berhampur University for providing the necessary facilities to carry out this research work.

Funding

The authors are thankful to MoEF & CC, Govt. India (F.n. No. 22018/19/2015-Cs (TAX) to carry out this piece of research work.

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Authors and Affiliations

  1. Algal Biotechnology and Molecular Systematic Laboratory, Post Graduate Department of Botany, Berhampur University, Bhanja Bihar, Berhampur, 760007, India

    Biswajita Pradhan, Rabindra Nayak, Chhandashree Behera & Mrutyunjay Jena

  2. Department of Biotechnology, Sangmyung University, Seoul, 03016, Korea

    Biswajita Pradhan & Jang-Seu Ki

  3. Cancer and Cell Death Laboratory, Department of Life Science, National Institute of Technology, Rourkela, India

    Srimanta Patra

  4. Division of Microbiology and NCDs, ICMR-Regional Medical Research Centre, Bhubaneswar, Odisha, 751023, India

    Shasank S. Swain

  5. Department of Biochemistry, All India Institute of Medical Science, Ansari Nagar, New Delhi, 110023, India

    Bimal Prasad Jit

  6. Department of Biological and Environmental Sciences and Technologies, Campus Ecotekne, University of Salento, via Monteroni, 73100, Lecce, Italy

    Andrea Ragusa

  7. CNR-Nanotec, Institute of Nanotechnology, via Monteroni, 73100, Lecce, Italy

    Andrea Ragusa

Authors
  1. Biswajita Pradhan
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  2. Srimanta Patra
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  3. Rabindra Nayak
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  7. Andrea Ragusa
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  8. Jang-Seu Ki
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  9. Mrutyunjay Jena
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Contributions

Biswajita Pradhan: Conceptualization, Methodology, Validation, Formal analysis, Investigation, Resources, Writing - original draft, Writing - review & editing, figure editing, Visualization, Supervision, Proof correction. Srimanta Patra: Formal analysis, review & editing. Rabindra Nayak: Methodology, review & figure editing. Shasank S. Swain: Formal analysis. Bimal Prasad Jit: Formal analysis. Chhandashree Behera: formal analysis. Andrea Ragusa: Formal analysis. Jang-Seu Ki: Formal analysis. Mrutyunjay Jena: Conceptualization, Methodology, Validation, Investigation, Resources, Review & editing, Supervision, Proof correction.

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Correspondence to Mrutyunjay Jena.

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Fig. S1.

The physiological and biochemical parameters after Cd, GR, and CdGR treatment of the C. reinhardtii strains as determined by principal component analysis (PCA). (PNG 388 kb)

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Pradhan, B., Patra, S., Nayak, R. et al. Low-dose priming of gamma radiation enhanced cadmium tolerance in Chlamydomonas reinhardtii by modulating physio-biochemical pathways. Environ Sci Pollut Res 29, 80383–80398 (2022). https://doi.org/10.1007/s11356-022-21374-9

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