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Advances in multiplex molecular detection technologies for harmful algae

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Abstract

As the eutrophication of natural water bodies becomes more and more serious, the frequency of outbreaks of harmful algal blooms (HABs) mainly formed by harmful algae also increases. HABs have become a global ecological problem that poses a serious threat to human health and food safety. Therefore, it is extremely important to establish methods that can rapidly detect harmful algal species for early warning of HABs. The traditional morphology-based identification method is inefficient and inaccurate. In recent years, the rapid development of molecular biology techniques has provided new ideas for the detection of harmful algae and has become a research hotspot. The current molecular detection methods for harmful algal species mainly include fluorescence in situ hybridization, sandwich hybridization, and quantitative PCR (qPCR), but all of these methods can only detect single harmful algal species at a time. The establishment of methods for the simultaneous detection of multiple harmful algal species has become a new trend in the development of molecular detection technology because various harmful algal species may coexist in the natural water environment. The established molecular techniques for multiple detections of harmful algae mainly include gene chip, multiplex PCR, multiplex qPCR, massively parallel sequencing, antibody chip, and multiple isothermal amplification. This review mainly focuses on the principles, advantages and disadvantages, application progress, and application prospects of these multiple detection technologies, aiming at providing effective references not only for the fisheries but also for economic activities, environment, and human health.

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Acknowledgements

This work was supported by the Shandong Provincial Natural Science Foundation, China (ZR2020MD081); the National Scientific Foundation of China (no. 31600309, 41476086); HIT Scientific Research Innovation Fund/the Fundamental Research Funds for the Central Universities (no. HIT.NSRIF.201702 and HIT.NSRIF.201709); HIT Environment and Ecology Innovation Special Funds (no. HSCJ201622).

Funding

This work was supported by the Shandong Provincial Natural Science Foundation, China (ZR2020MD081); the National Scientific Foundation of China (no. 31600309, 41476086); HIT Scientific Research Innovation Fund/the Fundamental Research Funds for the Central Universities (no. HIT.NSRIF.201702 and HIT.NSRIF.201709); HIT Environment and Ecology Innovation Special Funds (no. HSCJ201622).

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  1. College of Oceanology, Harbin Institute of Technology (Weihai), Weihai, 264209, People’s Republic of China

    Hanyu Fu, Chunyun Zhang, Yuanyuan Wang & Guofu Chen

  2. School of Environment, Harbin Institute of Technology, Harbin, 150009, People’s Republic of China

    Guofu Chen

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Conceived and designed the paper: Chunyun Zhang and Guofu Chen; Analysis and arrangement of documents: Yuanyuan Wang and Hanyu Fu; Wrote the paper: Hanyu Fu.

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Correspondence to Guofu Chen.

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Fu, H., Zhang, C., Wang, Y. et al. Advances in multiplex molecular detection technologies for harmful algae. Environ Sci Pollut Res 29, 43745–43757 (2022). https://doi.org/10.1007/s11356-022-20269-z

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