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Oral Nigella sativa oil administration alleviates arsenic-induced redox imbalance, DNA damage, and metabolic and histological alterations in rat liver

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Abstract

Arsenic, an omnipresent environmental contaminant, is regarded as a potent hepatotoxin. Nigella sativa oil (NSO) consumption has been shown to improve hepatic functions in various in vivo models of acute hepatic injury. The present study evaluates the protective efficacy of NSO against sodium arsenate (As)–induced deleterious alterations in the liver. Male Wistar rats were divided into four groups, namely, control, As, NSO, and AsNSO. After pre-treating rats in AsNSO and NSO groups with NSO (2 mL/kg bwt, orally) for 14 days, NSO treatment was further extended for 30 days, with and without As treatment (5 mg/kg bwt, orally), respectively. As induced an upsurge in serum ALT and AST activities indicating liver injury, as also confirmed by the histopathological findings. As caused significant alterations in the activities of membrane marker enzymes and carbohydrate metabolic enzymes, and in the vital components of antioxidant defense system. Marked DNA damage and hepatic arsenic accumulation were also observed in As-treated rats. Oral NSO administration ameliorated these deleterious alterations and improved overall hepatic antioxidant and metabolic status in As-treated rats. Prevention of oxidative damage could be the underlying mechanism of NSO-mediated protective effects. The results suggest that NSO could be a useful dietary supplement in the management of arsenic hepatotoxicity.

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Acknowledgements

Financial support to the Department of Biochemistry from the UGC-SAP-DRS III, DST-FIST, and DBT-PURSE programs is gratefully acknowledged. TA is the recipient of a senior research fellowship from DBT (Department of Biotechnology), Government of India. CIL, Central University of Punjab, Bhatinda, is acknowledged for providing the facilities for ICP-MS studies. Department of Chemistry, Aligarh Muslim University, Aligarh, is also acknowledged for providing the facilities for GC-MS studies.

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  1. Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, U.P., 202002, India

    Tauseef Alam, Sana Rizwan, Zeba Farooqui & Farah Khan

  2. Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, U.P., 202002, India

    Subuhi Abidi & Iqbal Parwez

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  1. Tauseef Alam
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Contributions

Conceived and designed the experiments: FK and TA; performed the experiments: TA and ZF; performed the histological experiment: IP and SA; analyzed the data: FK, TA, and SR; wrote the manuscript: TA; revised the manuscript: FK

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Correspondence to Farah Khan.

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Animal experiments were permitted by the Ministry of Environment, Forests and Climate Change, Government of India, under registration no. 714/GO/Re/02/CPCSEA issued by the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA) dated 29th October 2014 and approved by the Institutional Animal Ethics Committee (IAEC) of the Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India.

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Alam, T., Rizwan, S., Farooqui, Z. et al. Oral Nigella sativa oil administration alleviates arsenic-induced redox imbalance, DNA damage, and metabolic and histological alterations in rat liver. Environ Sci Pollut Res 28, 41464–41478 (2021). https://doi.org/10.1007/s11356-021-13493-6

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