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Quantitative proteomic analysis of tomato genotypes with differential cadmium tolerance

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Abstract

This is a report on comprehensive characterization of cadmium (Cd)-exposed root proteomes in tomato using label-free quantitative proteomic approach. Two genotypes differing in Cd tolerance, Pusa Ruby (Cd-tolerant) and Calabash Rouge (Cd-sensitive), were exposed during 4 days to assess the Cd-induced effects on root proteome. The overall changes in both genotypes in terms of differentially accumulated proteins (DAPs) were mainly associated to cell wall, redox, and stress responses. The proteome of the sensitive genotype was more responsive to Cd excess, once it presented higher number of DAPs. Contrasting protein accumulation in cellular component was observed: Cd-sensitive enhanced intracellular components, while the Cd-tolerant increased proteins of extracellular and envelope regions. Protective and regulatory mechanisms were different between genotypes, once the tolerant showed alterations of various protein groups that lead to a more efficient system to cope with Cd challenge. These findings could shed some light on the molecular basis underlying the Cd stress response in tomato, providing fundamental insights for the development of Cd-safe cultivars.

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Funding

This work was funded by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP—grants 2009/54676-0 and 2016/14349-3). This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES)—Finance Code 001. RAA also received research fellowship from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (grant 303749/2016-4).

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Authors and Affiliations

  1. Departamento de Genética, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Piracicaba, São Paulo, 13418-900, Brasil

    Karina Lima Reis Borges, Fernanda Salvato & Ricardo Antunes Azevedo

  2. Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, 13083-862, Brasil

    Fernanda Salvato

  3. W.M. Keck FTMS Laboratory for Human Health Research, Department of Chemistry, North Carolina State University, Raleigh, NC, 27695, USA

    Philip L. Loziuk & David C. Muddiman

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  1. Karina Lima Reis Borges
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  2. Fernanda Salvato
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  3. Philip L. Loziuk
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  4. David C. Muddiman
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  5. Ricardo Antunes Azevedo
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Correspondence to Ricardo Antunes Azevedo.

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Borges, K.L.R., Salvato, F., Loziuk, P.L. et al. Quantitative proteomic analysis of tomato genotypes with differential cadmium tolerance. Environ Sci Pollut Res 26, 26039–26051 (2019). https://doi.org/10.1007/s11356-019-05766-y

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