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Influence of nutrients on oxidation of low level methane by mixed methanotrophic consortia

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Abstract

Low-level methane emissions from coal mine ventilation air (CMV-CH4; i.e., 1 % CH4) can significantly contribute to global climate change, and therefore, treatment is important to reduce impacts. To investigate CMV-CH4 abatement potential, five different mixed methanotrohic consortia (MMCs) were established from soil/sediment sources, i.e., landfill top cover soil, bio-solid compost, vegetated humus soil, estuarine and marine sediments. Enrichment conditions for MMCs were as follows: nitrate mineral salt (NMS) medium, pH ~ 6.8; 25 °C; 20–25 % CH4; agitation 200 rpm; and culture period 20 days, in mini-bench-top bioreactors. The enriched cultures were supplemented with extra carbon (methanol 0.5–1.5 %, formate 5–15 mM, and acetate 5–15 mM), nitrogen (nitrate 0.5–1.5 g L−1, ammonium 0.1–0.5 g L−1, or urea: 0.1–0.5 g L−1), and trace elements (copper 1–5 μM, iron 1–5 μM, and zinc 1–5 μM) in different batch experiments to improve low-level CH4 abatement. Average CH4 oxidation capacities (MOCs) of MMCs varied between 1.712 ± 0.032 and 1.963 ± 0.057 mg g−1DWbiomass h−1. Addition of formate improved the MOCs of MMCs, but the dose-response varied for different MMCs. Acetate, nitrate and copper had no significant effect on MOCs, while addition of methanol, ammonium, urea, iron and zinc impacted negatively. Overall, MMCs enriched from marine sediments and landfill top cover soil showed high MOCs which were largely resilient to nutrient supplementation, suggesting a strong potential for biofilter development for industrial low-level CH4 abatement, such as those present in CMV.

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Acknowledgments

The project is supported by the Advanced Manufacturing Cooperative Research Centre (AMCRC), funded through the Australian Government’s Cooperative Research Centre Scheme, grant number 2.3.4. The funders had no role in study design, data collection and analysis or preparation of the manuscript and have provided permission to publish. This research is part of the MBD Energy Research and Development program for Biological Carbon Capture and Storage. We thank Townsville City Council and McCahills Landscaping Supplies for providing the soil samples from Stuart Landfill and Composting Facilities, respectively. KCP was supported by an AMCRC PhD fellowship at James Cook University. The authors appreciate the technical support in the operation of the Cirrus 2 and GC by SN and Mr. Shane Askew, respectively.

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  1. College of Marine and Environmental Sciences, James Cook University, Townsville, 4811, Queensland, Australia

    Obulisamy Parthiba Karthikeyan, Karthigeyan Chidambarampadmavathy, Saravanan Nadarajan & Kirsten Heimann

  2. Comparative Genomics Centre, James Cook University, Townsville, 4811, Queensland, Australia

    Obulisamy Parthiba Karthikeyan & Kirsten Heimann

  3. Centre for Bio-discovery and Molecular Development of Therapeutics, James Cook University, Townsville, 4811, Queensland, Australia

    Kirsten Heimann

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  1. Obulisamy Parthiba Karthikeyan
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Mixed methanotrophic consortium biomass yields of different soil/sediment types under 20 and 1 % methane enrichment (PDF 92.9 kb)

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Karthikeyan, O.P., Chidambarampadmavathy, K., Nadarajan, S. et al. Influence of nutrients on oxidation of low level methane by mixed methanotrophic consortia. Environ Sci Pollut Res 23, 4346–4357 (2016). https://doi.org/10.1007/s11356-016-6174-7

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