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Independent QTL underlie resistance to the native pathogen Quambalaria pitereka and the exotic pathogen Austropuccinia psidii in Corymbia

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Abstract

Fungal diseases such as the exotic myrtle rust (Austropuccinia psidii), and the native Quambalaria shoot blight (QSB; caused by Quambalaria species including Q. pitereka), constitute a significant threat to both native forests and Corymbia plantations in Australia and overseas. We here use quantitative trait loci (QTL) analysis to understand the genetic architecture of resistance to these pathogens in C. torelliana and C. citriodora subsp. variegata. QTL analysis was undertaken using 360 genotypes from two F1 crosses of C. torelliana × C. citriodora subsp. variegata, phenotyped by controlled inoculation with the strain of A. psidii present in Australia and independent inoculations with two strains of Q. pitereka (QSB1 & QSB2). A total of 22 QTL were identified, six for rust and 16 for QSB. The QTL for resistance to A. psidii and Q. pitereka in these pedigrees were independent from one another since they were generally in different parts of the genome, with only one case of co-location (QTL peak location within ± 2 MB). The QTL for the different QSB strains all mapped to discrete locations. The QTL for QSB were generally of a greater effect size than those for A. psidii. Several co-locations with QTL for resistance to rust and other fungal pathogens found in another eucalypt, Eucalyptus globulus, were detected and the implications of this observation are discussed.

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Acknowledgements

The authors wish to thank Ben Gosney for his advice regarding methods to evaluate QTL co-location, Mervyn Shepherd for his contributions to the genetic mapping of the Corymbia cross and DAF staff John Oostenbrink and Tracey Menzies for field and nursery work associated with the study.

Funding

This work was supported by the Australian Research Council (grant numbers DP140102552, DP110101621) and an Australian Government Research Training Program Scholarship.

Data archiving statement

New marker sequences are presented in the Online Resources. The Corymbia citriodora subsp. variegata genome assemblies will be published in the Comparative Genomics database (https://genomevolution.org/coge/) when the associated paper is prepared.

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Authors and Affiliations

  1. School of Natural Sciences, University of Tasmania, Private Bag 55, Hobart, TAS, 7001, Australia

    Jakob B. Butler, Brad M. Potts & René E. Vaillancourt

  2. ARC Training Centre for Forest Value, University of Tasmania, Private Bag 55, Hobart, TAS, 7001, Australia

    Brad M. Potts, René E. Vaillancourt & Jules S. Freeman

  3. Forest Industries Research Centre, University of the Sunshine Coast, Maroochydore DC, QLD, 4558, Australia

    David J. Lee

  4. Department of Agriculture and Fisheries, Ecosciences Precinct, GPO Box 267, Brisbane, QLD, 4001, Australia

    Geoff S. Pegg

  5. Scion, Private Bag 3020, Rotorua, 3046, New Zealand

    Jules S. Freeman

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  1. Jakob B. Butler
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Correspondence to Jules S. Freeman.

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Communicated by C. Kulheim

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Butler, J.B., Potts, B.M., Vaillancourt, R.E. et al. Independent QTL underlie resistance to the native pathogen Quambalaria pitereka and the exotic pathogen Austropuccinia psidii in Corymbia. Tree Genetics & Genomes 15, 72 (2019). https://doi.org/10.1007/s11295-019-1378-x

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