Abstract
Genetic mapping of quantitative trait loci (QTL) for resistance to Melampsora leaf rust was performed in two willow families: the progeny from a backcross between Salix viminalis and a hybrid S. viminalis × Salix schwerinii (population S1), and the F1 progeny of a cross between two S. viminalis (population S3). Disease levels were scored in the field for three consecutive years. In the laboratory, five different rust strains were sprayed onto leaf disks and the following resistance components were scored: latent period, diameter and number of uredinia, and flecking. One major QTL and 14 smaller were identified in the S1 host population. One rust strain, that represents a Melampsora form with limited incidence on S. viminalis, showed lower aggressiveness to the S1 host population together with a different pattern in QTLs. In the S3 host population, we detected 13 QTLs for rust resistance, of which two were located to the same genomic regions as those found for the S1 population. We showed that the strongest QTL co-segregated with a gene homologous to a known Toll/interleukin receptor-nucleotide binding site-leucine-rich repeat resistance gene in poplar. The importance of the identified QTLs is discussed in relation to breeding for durable resistance.
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Acknowledgment
We highly appreciate the assistance by Dr. Merje Toome, Solveig Höglund, and Marika Pettersson in the infection experiment work, the assistance by Urban Pettersson in the greenhouse work and by Dr. Katarina Ihrmark and Dr. Rena Gadjieva in the sequencing work. The study was supported by grants from the Swedish Energy Agency, Lantmännen Agroenergi AB and the Faculty of Natural Resources and Agricultural Sciences at the Swedish University of Agricultural Sciences.
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Communicated by W. Boerjan
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Supplementary Table 1
Disease resistance-like proteins in genomic regions of Populus trichocarpa corresponding to genomic regions of leaf rust resistance QTLs in the Salix genome (DOC 81 kb)
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Samils, B., Rönnberg-Wästljung, AC. & Stenlid, J. QTL mapping of resistance to leaf rust in Salix . Tree Genetics & Genomes 7, 1219–1235 (2011). https://doi.org/10.1007/s11295-011-0408-0
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DOI: https://doi.org/10.1007/s11295-011-0408-0