Abstract
Uptaking U(VI) from the environment by biological method is an environmental friendly and efficient way. In this work, we have acquired and isolated the protein DSR A by genetic engineering, then assessed its capacity and mechanisms to absorb U(VI) from wastewater. As results, we proved that protein DSR A can precisely recognize, enrich and remove uranyl ions in simulated wastewater solution. Its great removal potential was demonstrated in the adsorption experiments, the adsorption capacity of protein DSR A can reach 182.3 mg/g in the condition at 10 mg/L U(VI) and pH = 6. The Langmuir isotherm model and the pseudo-first-order kinetic equation were used to better describe the absorption process. We can confirm that Na+, Sr2+ and K+, these three metal ions have less effect on the enrichment of U(VI) by protein DSR A compared with other common cations. Besides, we can educe some mechanisms for the removal of U (VI) by protein DSR A from the results of FTIR, SEM–EDS, XPS (binding energy = 2.0 ~ 4.0ke V), MAP and XRD analysis before and after adsorption. This work has demonstrated the great potential of genetic engineering and biological methods in dealing with environmental heavy ion pollution.
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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (Grant number 11705085) and the Hunan Province Natural Science Foundation of China (Grant numbers 2020JJ4077 and 2020JJ6050) are gratefully acknowledged.
Funding
This work was supported by [National Natural Science Foundation of China] (Grant number [11705085]) and [Hunan Province Natural Science Foundation of China] (Grant numbers [2020JJ4077] and [2020JJ6050]).
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Material preparation, data collection and analysis were performed by [LC], [CC] and [KG]. The first draft of the manuscript was written by [YS] and [JX]. [GP] provided funding and technical guidance. All authors contributed to the study conception and design, all authors commented on previous versions of the manuscript, all authors read and approved the final manuscript.
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Shu, Y., Xie, J., Cheng, C. et al. Bio-enrichment of heavy metals U(VI) in wastewater by protein DSR A. World J Microbiol Biotechnol 38, 174 (2022). https://doi.org/10.1007/s11274-022-03362-w
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DOI: https://doi.org/10.1007/s11274-022-03362-w