Arsenic accumulation by a rhizosphere bacterial strain Ochrobactrum tritici reduces rice plant arsenic levels

Abstract

Arsenic naturally occurs in the earth’s crust and can be introduced in the environment by human activities. Agricultural practices in arsenic-contaminated environments pose a threat to human health. The contamination of crops contributes to the metalloid’s introduction in the food chain. This study aims to test the hypotheses that the inoculation of a hyperaccumulator rhizobacterial strain, Ochrobactrum tritici As5, to the rhizosphere of rice plants reduces the arsenic presence inside the tissue of the rice plants and reduces the inhibitory effect of the metalloid on the plant’s growth parameters. Inoculation of the hyperaccumulating strain O. tritici As5 showed the lowest concentration of arsenic in the plant’s tissue (2.6 fold lower than sterile plants), compared to the unmodified type O. tritici SCII24 and sterile rice plants. The inoculation of the type strain SCII24 also led to a decrease in arsenic concentration in the plant tissue compared with sterile plants (1.6 fold lower than sterile plants). The difference in arsenic presence in shoots was smaller among treatment groups than in the roots, showing a similar trend. The inoculation of the hyperaccumulator As5 strain alleviated some of the toxic effects of arsenic on shoot growth compared to inoculation of the unmodified type strain. All these findings together, contribute to our understanding of the interplay between arsenic pollution, plants and their rhizobacteria, especially the role of bioaccumulation of metal(oids) by rhizobacteria, and provide important information on the prevention of arsenic uptake by crops and the development of phytostabilizers.

Graphic abstract