Abstract
The effect of Tween 80 on the fermentative production of polymalic acid (PMA) and pullulan using Aureobasidium pullulans CCTCC M2012223 was investigated. Tween 80 is beneficial for the biosynthesis of PMA and pullulan, and can regulate the ratio of PMA to pullulan in a dose-dependent manner. After adding 0.05 % Tween 80 to the media, the maximal PMA and pullulan production was 46.45 and 28.8 g/L at 60 h in a 5 L fermenter, with an increase of 75.08 and 27.21 % when compared to the control. Tween 80 could regulate and enhance oxygen uptake rate and carbon dioxide evolution rate in the early phase of fermentation, and change the cell morphology. The transcription levels of mitochondrial dicarboxylate transporter and transmembrane transporter were also dramatically upregulated. The present work will be helpful in deeply understanding the mechanism of Tween 80 on the effect of PMA and pullulan production.
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References
Cheng KC, Demirci A, Catchmark JM (2011) Pullulan: biosynthesis, production, and applications. Appl Microbiol Biotechnol 92(1):29–44
Chi Z, Wang F, Chi Z, Yue L, Liu G, Zhang T (2009) Bioproducts from Aureobasidium pullulans, a biotechnologically important yeast. Appl Microbiol Biotechnol 82(5):793–804
Ding H, Portilla-Arias J, Patil R, Black KL, Ljubimova JY, Holler E (2011) The optimization of polymalic acid peptide copolymers for endosomolytic drug delivery. Biomaterials 32(22):5269–5278
Dionisio M, Cordeiro C, Remunan-Lopez C, Seijo B, Rosa da Costa AM, Grenha A (2013) Pullulan-based nanoparticles as carriers for transmucosal protein delivery. Eur J Pharm Sci 50(1):102–113
Duan X, Chi Z, Wang L, Wang X (2008) Influence of different sugars on pullulan production and activities of α-phosphoglucose mutase, UDPG-pyrophosphorylase and glucosyltransferase involved in pullulan synthesis in Aureobasidium pullulans Y68. Carbohydr Polym 73(4):587–593
Huypens P, Pillai R, Sheinin T, Schaefer S, Huang M, Odegaard ML, Ronnebaum SM, Wettig SD, Joseph JW (2011) The dicarboxylate carrier plays a role in mitochondrial malate transport and in the regulation of glucose-stimulated insulin secretion from rat pancreatic beta cells. Diabetologia 54(1):135–145
Kimmich GA, Randles J, Brand JS (1975) Assay of picomole amounts of ATP, ADP, and AMP using the luciferase enzyme system. Anal Biochem 69(1):187–206
Lanz-Landazuri A, Garcia-Alvarez M, Portilla-Arias J, de Ilarduya AM, Patil R, Holler E, Ljubimova JY, Munoz-Guerra S (2011) Poly(methyl malate) nanoparticles: formation, degradation, and encapsulation of anticancer drugs. Macromol Biosci 11(10):1370–1377
Lanz-Landazuri A, Garcia-Alvarez M, Portilla-Arias J, De Ilarduya AM, Holler E, Ljubimova J, Munoz-Guerra S (2012) Modification of microbial polymalic acid with hydrophobic amino acids for drug-releasing nanoparticles. Macromol Chem Phys 213(15):1623–1631
Leathers TD, Manitchotpisit P (2013) Production of poly(beta-l-malic acid) (PMA) from agricultural biomass substrates by Aureobasidium pullulans. Biotechnol Lett 35(1):83–89
Li BX, Zhang N, Peng Q, Yin T, Guan FF, Wang GL, Li Y (2009) Production of pigment-free pullulan by swollen cell in Aureobasidium pullulans NG which cell differentiation was affected by pH and nutrition. Appl Microbiol Biotechnol 84(2):293–300
Liu YS, Wu JY (2012) Effects of Tween 80 and pH on mycelial pellets and exopolysaccharide production in liquid culture of a medicinal fungus. J Ind Microbiol Biotechnol 39(4):623–628
Manitchotpisit P, Skory CD, Peterson SW, Price NP, Vermillion KE, Leathers TD (2012) Poly(beta-l-malic acid) production by diverse phylogenetic clades of Aureobasidium pullulans. J Ind Microbiol Biotechnol 39(1):125–132
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31(3):426–428
Prajapati VD, Jani GK, Khanda SM (2013) Pullulan: an exopolysaccharide and its various applications. Carbohydr Polym 95(1):540–549
Ruodong C, Hao W, Hua Z, Yuanyuan C, Ruoping H, Ping W (2012) Investigation of poly(β-malic acid) synthesis pathways and regulation by strains of Aureobasidium pulluans. CIESC J 63(11):3639–3644
Sheng L, Zhu G, Tong Q (2013) Mechanism study of Tween 80 enhancing the pullulan production by Aureobasidium pullulans. Carbohydr Polym 97(1):121–123
Silva CC, Dekker RFH, Silva RSSF, da Silva MDLC, Barbosa AM (2007) Effect of soybean oil and Tween 80 on the production of botryosphaeran by Botryosphaeria rhodina MAMB-05. Process Biochem 42(8):1254–1258
Sousa MJ, Mota M, Leao C (1992) Transport of malic acid in the yeast Schizosaccharomyces pombe: evidence for proton-dicarboxylate symport. Yeast 8(12):1025–1031
Thomsen LB, Lichota J, Kim KS, Moos T (2011) Gene delivery by pullulan derivatives in brain capillary endothelial cells for protein secretion. J Control Release 151(1):45–50
Valentini M, Storelli N, Lapouge K (2011) Identification of C-4-dicarboxylate transport systems in Pseudomonas aeruginosa PAO1. J Bacteriol 193(17):4307–4316
Yu X, Wang Y, Wei G, Dong Y (2012) Media optimization for elevated molecular weight and mass production of pigment-free pullulan. Carbohydr Polym 89(3):928–934
Zan Z, Zou X (2013) Efficient production of polymalic acid from raw sweet potato hydrolysate with immobilized cells of Aureobasidium pullulans CCTCC M2012223 in aerobic fibrous bed bioreactor. J Chem Technol Biotechnol 88(10):1822–1827
Zhang B-B, Cheung PCK (2011) A mechanistic study of the enhancing effect of Tween 80 on the mycelial growth and exopolysaccharide production by Pleurotus tuber-regium. Bioresour Technol 102(17):8323–8326
Zhang H, Cai J, Dong J, Zhang D, Huang L, Xu Z, Cen P (2011) High-level production of poly (beta-l-malic acid) with a new isolated Aureobasidium pullulans strain. Appl Microbiol Biotechnol 92(2):295–303
Zhang B-B, Chen L, Cheung PCK (2012) Two-dimensional gel electrophoresis analysis of mycelial cells treated with Tween 80: differentially expressed protein related to enhanced metabolite production. J Agric Food Chem 60(42):10585–10591
Zhao Z, Hu X, Ross CW (1987) Comparison of tissue preparation methods for assay of nicotinamide coenzymes. Plant Physiol 84(4):987–988
Zou X, Hang HF, Chu J, Zhuang YP, Zhang SL (2009) Oxygen uptake rate optimization with nitrogen regulation for erythromycin production and scale-up from 50 L to 372 m3 scale. Bioresour Technol 100(3):1406–1412
Zou X, Zhou Y, Yang ST (2013) Production of polymalic acid and malic acid by Aureobasidium pullulans fermentation and acid hydrolysis. Biotechnol Bioeng 110(8):2105–2113
Acknowledgments
This work was supported in part by grants from the National High Technology Research and Development Program of China (863 Program) (No. 2014AA021205), National Transformation Fund for Agricultural Science and Technology (2012F1003006), and Fundamental Research Funds for the Central Universities (XDJK2013B039 and 2362014XK07).
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11274_2014_1779_MOESM1_ESM.xlsx
Table S1: The sequence annotation of different expression gene with addition of different Tween 80 concentration in 5L stirred-tank fermenter (XLSX 12 kb)
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Tu, G., Wang, Y., Ji, Y. et al. The effect of Tween 80 on the polymalic acid and pullulan production by Aureobasidium pullulans CCTCC M2012223. World J Microbiol Biotechnol 31, 219–226 (2015). https://doi.org/10.1007/s11274-014-1779-9
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DOI: https://doi.org/10.1007/s11274-014-1779-9