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Improved growth of bait microalgae Isochrysis and aquacultural wastewater treatment with mixotrophic culture

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Abstract

This research of mixotrophic microalgae Isochrysis 3011 with glycerol was combined with the treatment of aqua-cultural wastewater, different initial concentrations, and optimized light intensities. The algae growth rate, removal efficiencies of total nitrogen (TN) and total phosphorus (TP) were determined. Results showed that the suitable initial concentration was 0.4 g L−1, and the optimum light intensity was 60 µmol m−2 s−1. The growth of the mixotrophic group was better than that of the autotrophic culture. The biomass yield of the mixotrophic group with glycerol was 0.17 g L−1 d−1, and the removal rates of TN and TP were 73.39% and 95.61%, respectively. The content of total lipid and total protein in mixotrophic group were higher than the values of the autotrophic group. This indicates that aquaculture wastewater treatment with mixotrophic bait microalgae can obtain superior micro-algal biomass, which is also a potential technology for wastewater utilization and ecological protection.

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Acknowledgements

This research was supported in part by grants from the National Key Research and Development Program of China (2018YFD0901504), the National Natural Science Foundation of China (32170369), the State Key Laboratory of Marine Resource Utilization in South China Sea (Hainan University) (MRUKF2021003), the Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences (KF2019NO3), the Natural Science Foundation of Zhejiang Province (LY20D060003), the Fundamental Research Funds for the Provincial Universities (SJLY2020007), Ningbo Science and Technology Research Projects, China (2019C10023), the General Research Project of Zhejiang Provincial Department of Education (Y202045551), the Scientific Research Foundation of Graduate School of Ningbo University (IF2021081), and the Ningbo Municipal Science and Technology Project (2019C10071).

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Authors and Affiliations

  1. College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, 315211, Zhejiang, China

    Ruirui Chu, Chengxu Zhou & Pengfei Cheng

  2. State Key Laboratory of Marine Resource Utilization in South China Sea, College of Oceanology, Hainan University, Haikou, 570228, Hainan, China

    Jingxue Ma & Yandu Lu

  3. School of Resource and Environmental Engineering, Hubei University of Technology, Wuhan, Hubei, China

    Defu Liu

  4. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Guangce Wang

  5. Center for Biorefining, Department of Bioproducts and Biosystems Engineering, University of Minnesota-Twin Cities, Saint Paul, MN, 55108, USA

    Roger Ruan

  6. Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, 315211, Zhejiang, China

    Xiaojun Yan

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  1. Ruirui Chu
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Correspondence to Yandu Lu or Pengfei Cheng.

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Fig. S1

Effects of different carbon sources and its concentration on the growth of Isochrysis 3011. (PPTX 50 KB)

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Chu, R., Ma, J., Zhou, C. et al. Improved growth of bait microalgae Isochrysis and aquacultural wastewater treatment with mixotrophic culture. Bioprocess Biosyst Eng 45, 589–597 (2022). https://doi.org/10.1007/s00449-021-02681-w

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