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Alterations in the Development and Gonadal Structure of Nile Tilapia (Oreochromis niloticus) Exposed to Natural and Synthetic Estrogens

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Abstract

This study aimed to investigate the effects of endocrine disruptors 17β-estradiol (E2) and 17α-ethinylestradiol (EE2) on Nile tilapia (Oreochromis niloticus) development, focusing on gonadal histological factors. Concentrations of estrogens E2 and EE2 ranging from 250 to 1000 ng·L−1 can produce intersex individuals, significantly decreasing the condition factor as the concentrations increased. These concentrations could also induce the development of morphological anomalies. Increasing the concentration of estrogens by one order of magnitude (µg·L−1) was lethal for Nile tilapia larvae, with no effect on the incubation time and percentage of larvae hatching. Additionally, morphological anomalies and developmental problems were observed. Estrogens applied at a concentration of 160 µg·L−1 for 28 days caused the birth of a small number of intersex individuals in Nile tilapia, but generated almost entirely female populations in hormonal treatments. Furthermore, the gonads of fish exposed to the estrogens were smaller and less developed, without any significant difference between E2 and EE2. Variation in the exposure time (one to 4 weeks) of Nile tilapia to estrogens (160 µg·L−1) resulted in the appearance of intersex individuals and the development of morphological anomalies, regardless of the exposure time.

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Funding

The authors would like to thank the National Council for Scientific and Technological Development (CNPq), Process nº 481985/2012–3, for the financial support.

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  1. Department of Hydraulic and Environmental Engineering, Federal University of Ceará, Campus do Pici, Bloco 713. Pici. CEP, Fortaleza, Ceará, 60455-900, Brazil

    Oscar Pacheco Passos Neto, André Bezerra dos Santos & Suetônio Mota

  2. Department of Biology, Federal University of Ceará, Fortaleza, Ceará, Brazil

    José Roberto Feitosa Silva

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  1. Oscar Pacheco Passos Neto
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  2. André Bezerra dos Santos
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Pacheco Passos Neto, O., Bezerra dos Santos, A., Feitosa Silva, J.R. et al. Alterations in the Development and Gonadal Structure of Nile Tilapia (Oreochromis niloticus) Exposed to Natural and Synthetic Estrogens. Water Air Soil Pollut 232, 448 (2021). https://doi.org/10.1007/s11270-021-05375-x

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