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Transcriptomic analyses provide insight into adventitious root formation of Euryodendron excelsum H. T. Chang during ex vitro rooting

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Abstract

Euryodendron excelsum H. T. Chang, a critically endangered species endemic to China, is a source of valuable material for the furniture and construction industries. However, this species has some challenges associated with rooting during in vitro propagation that have yet to be resolved. In this study, we optimized rooting and conducted a transcriptomic analysis to appreciate its molecular mechanism, thereby promoting the practical application of in vitro propagation of E. excelsum, and providing technical support for the ecological protection of this rare and endangered species. Results showed that ex vitro rooting performed the highest rooting percentage with 98.33% at 25 days. During ex vitro rooting, there was a wide fluctuation of endogenous levels of indole-3-acetic acid (IAA) and hydrogen peroxide (H2O2) at the stage of root primordia formation. Transcriptome analysis revealed multiple differentially expressed genes (DEGs) involved in adventitious root (AR) development. DEGs involved in plant hormone signal transduction, such as genes encoding auxin-induced protein, auxin-responsive protein, and auxin transporter-like protein, and in response to H2O2, oxidative stress, abiotic and biotic stimuli were significantly up- or down-regulated by ex vitro treatment with 1 mM indole-3-butyric acid (IBA). Our results indicate that ex vitro rooting is an effective method to induce AR from E. excelsum plantlets during micropropagation. DEGs involved in the plant hormone signal transduction pathway played a crucial role in AR formation. H2O2, produced by environmental stimulation, might be related to AR induction as a result of the synergistic action with IBA, ultimately regulating the level of endogenous IAA.

Key message

Under ex vitro rooting, a synergistic action between H2O2 produced by environmental stimulation and IBA played crucial role in the regulation of AR formation from E. excelsum plantlets during micropropagation.

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Data availability

All data generated or analyzed during this study are included in this published article and its supplementary information files. The RNA-seq data has been deposited in the Sequence Read Archives Database (https://www.ncbi.nlm.nih.gov/sra/) under accession number PRJNA723111 (http://www.ncbi.nlm.nih.gov/bioproject/723111).

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Acknowledgements

We thank Personal Biotechnology Co., Ltd. for their skillful support of RNA-sequencing.

Funding

This work was supported by the National Natural Science Foundation of China Youth Fund (32100311), National Key Research Plan of China (Grant No.: 2016YFC0503104) and Guangdong Province Science and Technology Program (Number: 2015B020231008).

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Author notes

  1. Yuping Xiong and Shuangyan Chen have contributed equally to this work.

Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, China

    Yuping Xiong, Xiaohong Chen, Beiyi Guo, Ting Zhang, Yuying Yin, Jinhui Pang, Meiyun Niu, Xinhua Zhang, Yuan Li, Kunlin Wu, Lin Fang, Guohua Ma & Songjun Zeng

  2. University of Chinese Academy of Sciences, Beijing, 100039, China

    Yuping Xiong, Xiaohong Chen, Beiyi Guo, Ting Zhang, Yuying Yin, Jinhui Pang & Meiyun Niu

  3. Institute of Nanfan & Seed Industry, Guangdong Academy of Sciences, Guangdong, 510316, China

    Shuangyan Chen

  4. Zhanjiang Sugarcane Research Center, Guangzhou Sugarcane Industry Research Institute, Zhanjiang, 524300, Guangdong, China

    Shuangyan Chen

  5. Zhongkai University of Agriculture and Engineering, Guangzhou, 510650, China

    Zhenpeng Wei & Xincheng Yu

  6. Independent researcher, P.O. Box 7, Miki-cho, Ikenobe 3011-2, Kagawa-ken, Takamatsu, 761-0799, Japan

    Jaime A. Teixeira da Silva

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  1. Yuping Xiong
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Contributions

XHZ, YL, KLW, FL, JATdS, GHM and SJZ designed the experiment and provided guidance on the study. YPX and SYC prepared samples for AR induction and RNA-seq analysis. XHC, TZ, BYG and MYN performed the statistical analysis on the determination of IAA and H2O2 content, and RNA-seq data. ZPW, YYY, XCY and JHP participated in the experiment of AR induction and anatomical analysis. YPX, JATdS and SYC were involved in statistical analyses and co-wrote the manuscript. All authors wrote, read and approved the manuscript.

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Correspondence to Guohua Ma or Songjun Zeng.

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Communicated by Paloma Moncaleán.

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Xiong, Y., Chen, S., Wei, Z. et al. Transcriptomic analyses provide insight into adventitious root formation of Euryodendron excelsum H. T. Chang during ex vitro rooting. Plant Cell Tiss Organ Cult 148, 649–666 (2022). https://doi.org/10.1007/s11240-021-02226-9

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