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Cell culture system versus adventitious root culture system in Asian and American ginseng: a collation

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Abstract

Panax ginseng and Panax quinquefolius of Panax genus are valuable as health foods as well as pharmaceuticals for the treatment of cancer, diabetes and ageing as these plants possess saponins. In the current study, Cell and adventitious root cultures of P. ginseng and P. quinquefolius were investigated for the biomass, cell division, saponin content and ginsenosides profile from four lines namely P. quinquefolius (AM), P. ginseng mountain (Mt.) Baekdu line, P. ginseng Cheong-sol line (CS) and P. ginseng CBN line (CBN) with the objective of comparing cell and adventitious root systems to check their efficacy for the production of ginseng saponins. Additionally, genes related to ginsenoside biosynthesis were also analyzed concerning to cell and adventitious root lines. The results indicated that various cell lines were better in multiplication and growth compared to adventitious root lines. However, adventitious root lines showed higher accumulation of dry biomass (1.5–2 fold) than that of cell lines. CS adventitious root line showed higher saponin content and ginsenoside productivity (10.48 mg·g−1 DW, 12.88 mg·L−1, respectively) than that of CS cell line (9.50 mg·g−1 DW, 2.39 mg·L−1, respectively). Especially, Rd ginsenoside productivity of CS adventitious root line recorded fourfold higher than CS cell line. Genes which are related to ginsenoside biosynthesis such as P. ginseng squalene synthase (PgSS2), P. ginseng squalene epoxidase (PgSE2), P. ginseng protopanaxadial synthase (PgPPDS) and P. ginseng protopanaxatriol synthase (PgPPTS) were analyzed by real time quantitative polymerase chain reaction to support ginsenoside production. The adventitious root culture system described in this study is useful system for biomass and ginsenoside production.

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References

  • Abe I, Rohmer M, Prestwich GD (1993) Enzymatic cyclization of squalene and odidosquaelene to sterols and triterpenes. Chem Rev 93:2189–2206

    Article  CAS  Google Scholar 

  • Akalezi CO, Liu S, Li QS, Yu JT, Zhong JJ (1999) Combined effects of initial sucrose concentration and inoculums size on cell growth and ginseng saponin production by suspension cultures of Panax ginseng. Process Biochem 34:639–642

    Article  CAS  Google Scholar 

  • Furuya T, Yoshikawa T, Kajii K (1983a) Effect of auxin on growth and saponin production in callus cultures of Panax ginseng. Planta Med 47:183–187

    Article  CAS  PubMed  Google Scholar 

  • Furuya T, Yoshikawa T, Orihara Y, Oda H (1983b) Saponin production in cell suspension cultures of Panax ginseng. Planta Med 48:83–87

    Article  CAS  PubMed  Google Scholar 

  • Han JY, Kim HJ, Kwon YS, Choi YE (2011) The Cyt P450 CYP716A47 catalyzes the formation of protopanaxadiol from dammarenediol-II during ginsenoside biosynthesis in Panax ginseng. Plant Cell Physiol 52:2062–2073

    Article  CAS  PubMed  Google Scholar 

  • Han JY, Hwang HS, Choi SW, Kim HJ, Choi YE (2012) Cytochrome P450 CYP716A53v2 catalyzes the formation of protopanaxatriol from protopanaxadiol during ginsenoside biosynthesis in Panax ginseng. Plant Cell Physiol 53:1543–1545

    Article  Google Scholar 

  • Hwang CR, Lee SH, Jang GY, Hwang IG, Kim HY, Woo KS, Lee J, Jeong HS (2014) Changes in ginsenoside compositions and antioxidant activities of hydroponic-cultured ginseng roots and leaves with heating temperature. J Ginseng Res 38:180–186

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jeong GT, Park DH, Ryu HW, Hwang B, Woo JC, Kim D, Kim SW (2005) Production of antioxidant compounds by culture of Panax ginseng C. A. Meyer hairy roots. Appl Biochem Biotechnol 121–124:1147–1157

    Article  PubMed  Google Scholar 

  • Kim YJ, Zhang D, Yang DC (2015) Biosynthesis and biotechnological production of ginsenosides. Biotechcnol Adv 33:717–735

    Article  CAS  Google Scholar 

  • Kiselev KV, Shumakova OA, Tchernoded GK (2011) Mutation of Panax ginseng genes during long-term cultivation of ginseng cell cultures. J Plant Physiol 168:1280–1285

    Article  CAS  PubMed  Google Scholar 

  • Kiselev KV, Dubrovina AS, Shumakova OA (2013) DNA mutagenesis in 2- and 20-yr-old Panax ginseng cell cultures. In Vitro Cell Dev Biol Plant 49:175–182

    Article  CAS  Google Scholar 

  • Lemontey C, Mousset-Declass C, Munier-Jolain N, Boutin JP (2000) Maternal genotype influences pea seed size by controlling both mitotic activity during early embryogenesis and final endoreduplication level/cotyledon cell size in mature seed. J Exp Bot 51:167–175

    Article  CAS  PubMed  Google Scholar 

  • Liang LF, Keng CL, Lim BP (2006) Selection of cell lines for production of rosamarinic acid from cell suspension cultures of Orthosiphon stamineus Benth. In Vitro Cell Dev Biol Plant 42:538–542

    Article  CAS  Google Scholar 

  • Liu S, Zhong JJ (1998) Phosphate effect on production ginseng saponin and polysaccharide by cell suspension cultures of Panax ginseng and Panax quninquefolium. Process Biochem 33:69–74

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Murthy HN, Hahn EJ, Paek KY (2008) Adventitious roots and secondary metabolism. Chin J Biotech 24:711–716

    Article  CAS  Google Scholar 

  • Murthy HN, Georgiev MI, Kim YS, Jeong CS, Kim SJ, Park SY, Paek KY (2014a) Ginsenosides: perspective for sustainable biotechnological production. Appl Microbiol Biotechnol 98:6243–6254

    Article  CAS  PubMed  Google Scholar 

  • Murthy HN, Lee EJ, Paek KY (2014b) Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation. Plant Cell Tissue Organ C 118:1–16

    Article  CAS  Google Scholar 

  • Murthy HN, Dandin VS, Paek KY (2016) Tools for biotechnological production of useful phytochemicals from adventitious root cultures. Phytochem Rev 15:127–145

    Article  Google Scholar 

  • Obae SG, West TP (2012) Nuclear DNA content and genome size of American ginseng. J Med Plants Res 6:4719–4723

    Article  CAS  Google Scholar 

  • Paek KY, Murthy HN, Hahn EJ, Zhong JJ (2009) Large scale culture of ginseng adventitious roots for production of ginsenosides. Adv Biochem Eng Biotechnol 113:151–176

    CAS  PubMed  Google Scholar 

  • Park JD, Rhee DK, Lee YH (2005) Biological activities and chemistry of saponins from Panax ginseng C. A. Meyer. Phytochem Rev 4:159–175

    Article  CAS  Google Scholar 

  • Payne GF, Bringi V, Prince C, Shuler ML (1991) The quest for commercial production of chemicals from plant cell culture. In: Payne GF, Bringi V, Prince C, Shuler ML (eds) Plant cell and tissue culture in liquid systems. Hanser, Munich

    Google Scholar 

  • Shi FX, Li MR, Li YL, Jiang P, Zhang C, Pan YZ, Liu B, Xiao HX, Li LF (2015) The impact of polyploidy, geographic and ecological isolations on the diversification of Panax (Araliaceae). BMC Plant Biol 15:297

    Article  PubMed  PubMed Central  Google Scholar 

  • Thanh NT, Murthy HN, Paek KY (2014) Ginseng cell culture for production of ginsenosides. In: Paek KY, Murthy HN, Zhong JJ (eds) Production of biomass and bioactive compounds using bioreactor technology. Springer, Dordrecht

    Google Scholar 

  • Vincken JP, Heng L, de Groot A, Gruppen H (2007) Saponins, classification and occurrence in the plant kingdom. Phytochemistry 68:275–297

    Article  CAS  PubMed  Google Scholar 

  • Waminal NE, Park HM, Ruy KB, Kim JH, Yang TJ, Kim HH (2012) Karyotype analysis of Panax ginseng C. A. Meyer, 1843 (Araliaceae) based on rDNA loci and DAPI band distribution. Comp Cytogenet 6:425–441

    Article  PubMed  PubMed Central  Google Scholar 

  • Wu J, Zhong JJ (1999) Production of ginseng and its bioactive components in plant cell culture: current biotechnological and applied aspects. J Biotechnol 68:89–99

    Article  CAS  PubMed  Google Scholar 

  • Yu KW, Hahn EJ, Paek KY (2000) Production of adventitious ginseng roots using bioreactors. J Plant Biotechnol 27:309–315

    Google Scholar 

Download references

Acknowledgements

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Advanced Production Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (Grant Number 315013-4).

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Authors and Affiliations

  1. Division of Animal, Horticulture and Food Sciences, Department of Horticultural Science, Chungbuk National University, Ch’ongju, 28644, Republic of Korea

    Jong-Du Lee, Kim-Cuong Le, Hosakatte Niranjana Murthy, Kee-Yoeup Paek & So-Young Park

  2. Department of Forest Genetic Resources, National Institute of Forest Science, 39 Onjeong-ro, Suwon, 16631, Republic of Korea

    Young-Ki Park

  3. Department of Botany, Karnatak University, Dharwad, 580003, India

    Hosakatte Niranjana Murthy

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  1. Jong-Du Lee
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  2. Kim-Cuong Le
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  3. Young-Ki Park
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  4. Hosakatte Niranjana Murthy
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  5. Kee-Yoeup Paek
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Contributions

J-DL, KCL, and YKP contribute for acquisition data and writing of manuscript. HNM, and K-YP participated in interpreted data and revising for important intellectual content. S-YP made substantial contributions to conception and design this study.

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Correspondence to Hosakatte Niranjana Murthy or So-Young Park.

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The authors declare that they have no conflicts of interest.

Additional information

Communicated by Sergio J. Ochatt.

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Lee, JD., Le, KC., Park, YK. et al. Cell culture system versus adventitious root culture system in Asian and American ginseng: a collation. Plant Cell Tiss Organ Cult 132, 295–302 (2018). https://doi.org/10.1007/s11240-017-1329-x

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  • DOI: https://doi.org/10.1007/s11240-017-1329-x

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