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New Achievement in Panax vietnamensis Research

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Biotechnology of Neglected and Underutilized Crops

Abstract

Panax vietnamensis Ha et Grushv., an endemic Panax genus of Vietnam, is a well known Vietnamese ginseng (Ngoc Linh ginseng) rich in pharmaceutical compounds, most importantly saponin. Its cultivation takes a long time, generally 5–7 years, and needs extensive efforts to quality control in the face of environmental stresses including soil, shade, climate, pathogens and pests. In vitro techniques have been widely explored for rapid and efficient production of ginseng biomass and ginsenosides. The establishment of cell and adventitious root cultures of P. vietnamensis opens the way to commercial applications. Various physiological and biochemical parameters affecting the biomass production and ginsenoside accumulation have been investigated. These parameters are effect of various phytohormones, sucrose and activated charcoal (AC) on shoot regeneration and proliferation from callus, and adventitious and secondary root formation. The saponin analysis of calli and roots showed the presence of ginsenoside-Rg1, majonoside-R2, and ginsenoside-Rb1. These results indicated that P. vietnamensis biomass has a great potential to produce saponin as a new source for the pharmaceutical and cosmetic industry.

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Abbreviations

AC:

Activated charcoal

MS:

Murashige and Skoog

NAA:

α-napthaleneacetic acid

2, 4-D:

2, 4-dichlorophenoxyacetic acid

TDZ:

Thidiazuron

IBA:

Indole-3-butyric acid

References

  • Akalezi CO, Liu S, Li QS, Yu JT, Zhong JJ (1999) Combined effects of initial sucrose concentration and inoculum size on cell growth and ginseng saponin production by suspension cultures of Panax ginseng. Process Biochem 34:639–642

    Article  CAS  Google Scholar 

  • Arya S, Arya IDI, Eriksson T (1993) Rapid multiplication of adventitious somatic embryos of Panax ginseng. Plant Cell Tiss Org Cul 34:157–162

    Article  CAS  Google Scholar 

  • Bonfill M, Rosa MC, Javier P, Teresa PM, Morales C (2002) Influence of auxins on organogenesis and ginsenoside production in Panax ginseng calluses. Plant Cell Tiss Org Cul 68:73–78

    Article  CAS  Google Scholar 

  • Dong NT, Luan TC, Huong NTT (2007) Ngoc Linh Ginseng and some medicinal plants belong to Ginseng family. Science and Technology Publishing House, Vietnam

    Google Scholar 

  • Dung NN (1995) Propagation of Panax vietnamensis by biological technology. Agricultural Publishing Houses, Vietnam

    Google Scholar 

  • Duong VB, Dao Don DV, Long NV, Luong HV, Lau TV (2008) Study on quantitative analysis of some main ginsenosides of Ngoc Linh Ginseng by HPLC method. Med J 390:41–43

    Google Scholar 

  • Furuya T, Yoshikawa T, Ushiyama K, Oda H (1986) Formation of plantlets from callus cultures of ginseng (Panax ginseng). Experientia 42:193–194

    Article  CAS  Google Scholar 

  • Garcia FG, John WE (1983) Ethylene and ethane production in 2,4-D treated and salt treated tobacco tissue cultures. Ann Bot 51:287–295

    CAS  Google Scholar 

  • George EF, Sheringtoln PD (1984) Plant propagation by tissue culture. Exegetics Ltd., Eversley

    Google Scholar 

  • Jacques P, Kevers C, Gaspar T, Dommes J, Thonart P (2007) Condition Panax vietnamensis cell mass production in bioreactors. Acta Bot Gallica 154(1):21–26

    CAS  Google Scholar 

  • Kull T, Arditti J (2002) Orchid biology reviews and perspective, vol 8. Kluwer Academic Publishers, The Netherland, pp 443–487

    Book  Google Scholar 

  • Langhansová L, Maršík P, Vaněk T (2005) Production of saponins from Panax ginseng suspension and adventitious root cultures. Biol Plantarium 49(3):463–465

    Article  Google Scholar 

  • Lim HT, Lee HS (1997) Regeneration of Panax ginseng C. A. Meyer by organogenesis DNA analysis of regenerants. Plant Cell Tiss Org Cul 49:179–187

    Article  CAS  Google Scholar 

  • Medina M, Villalobos N, Cruz PJDL, Dorado A, Guerra H (1998) Effect of culture medium and light conditions on the morphological characteristics and carbohydrate contents of Medicago strasseri calli. Acta Physiol Plant 20(4):383–392

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nhut DT, Luan VQ, Binh NV, Phong PT, Huy BN, Ha DTN, Tam PQ, Nam NB, Hien VT, Vinh BT, Hang LTM, Ngoc DTM, Thao LB, Luan TC (2009a) The effects of some factors on in vitro biomass production of vietnamese ginseng (Panax vietnamensis ha et grushv.) and preliminary analysis of saponin content. J Biotechnol Viet 7(3):365–370

    Google Scholar 

  • Nhut DT, Tam PQ, Luan VQ, Binh NV, Ha DTN, Chien HX, Nhan NCT Nam NB, Truc NB, Thuy LNM, Nguyen PLH, Hien VT, Hang LTM, Hang NTT, Hai NT (2009b) Adventitious and secondary root formation of Panax vietnamensis cultured in vitro. In: Proceedings of biotechnology conference of southern provinces of Vietnam. Science and Technology Publishers, pp. 252–258

    Google Scholar 

  • Nhut DT, Hang LTM, Vinh BT, Tam PQ, Nam NB, Nhan NCT, Chien HX, Thuy LNMT, Hien VT, Binh NV, Luan VQ, Luan TC, Duc DT (2010) Identification of saponin content and residue of some phytohormones in callus, shoot and root of Panax vietnamensis cultured in vitro. J Biotechnol Viet 8(2):189–202

    Google Scholar 

  • Nhut DT, Huy NP, Luan VQ, Binh NV, Nam NB, Thuy LNM, Ha DTN, Chien HX, Huong TT, Cuong HV, Cuong LK, Hien VT (2011) Shoot regeneration and micropropagation of Panax vietnamensis Ha et Grushv. from ex vitro leaf-derived callus. Afr J Biotechnol 10(84):19499–19504

    CAS  Google Scholar 

  • Radhakrishnan T, Murthy TGK, Chandran K, Banyopadhyay A (2001) Somatic embryogenesis in Arachis hypogaea revisited. Aust J Bot 49:753–759

    Article  CAS  Google Scholar 

  • William EC (2000) Ginseng. Harwood Academic Publishers, The Gordon and Breach Publishing Group

    Google Scholar 

  • Zhang JT (2006) The chemistry, metabolism and biological activities of ginseng. Chemical Industry Press, Beijing, pp 3–17

    Google Scholar 

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Acknowledgments

The authors would like to thank the National Foundation for Science and Technology Development (Ministry of Science and Technology, Vietnam), the Committee for Local Science and Technology (Ministry of Science and Technology, Vietnam) and the Department of Application and Development of Technology (Vietnam Academy of Science and Technology) for the financial support.

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Authors and Affiliations

  1. Tay Nguyen Institute of Biology, 116 Xo Viet Nghe Tinh, Dalat, Lam Dong, Vietnam

    Duong Tan Nhut, Nguyen Thanh Hai, Nguyen Phuc Huy, Hoang Xuan Chien & Nguyen Ba Nam

Authors
  1. Duong Tan Nhut
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  2. Nguyen Thanh Hai
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  3. Nguyen Phuc Huy
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  4. Hoang Xuan Chien
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  5. Nguyen Ba Nam
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Correspondence to Duong Tan Nhut .

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Editors and Affiliations

  1. , Department of Agricultural, University of Helsinki, 5 Latokartanonkaari Room C 130, Helsinki, 00014, Finland

    Shri Mohan Jain

  2. Dept. Agricultural & Food Engineering, Indian Institute of Technology, Kharagpur, 721302, India

    S. Dutta Gupta

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Nhut, D.T., Hai, N.T., Huy, N.P., Chien, H.X., Nam, N.B. (2013). New Achievement in Panax vietnamensis Research. In: Jain, S., Dutta Gupta, S. (eds) Biotechnology of Neglected and Underutilized Crops. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5500-0_3

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