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Effects of virus infection on plant growth, root development and phytohormone levels in in vitro-cultured pear plants

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Abstract

Virus infection is common in pear (Pyrus spp.) trees commercially cultivated in China. In this study, the growth speed, root development and phytohormone levels of virus-free and virus-infected pear cultures were comparatively investigated. Results showed that the co-infection of Apple stem grooving virus (ASGV) and Apple stem pitting virus (ASPV) significantly decreased the growth and proliferation of in vitro plants of P. communis cv. ‘Confenence’, but showed relatively less effect for P. pyrifolia cv. ‘Jinshui no. 2’, P. communis cv. ‘Red Clapp Favonite’ and P. sinkiangensis cv. ‘Korla’. Strong inhibition of ASGV infection to root development was observed for in vitro plants of ‘Jinshui no. 2’, but not for ‘Confenence’. Furthermore, during root induction, ASGV infection significantly increased CTKs/IAA ratio and decreased IAA/ABA ratio in the rooting region of in vitro plants of ‘Jinshui no. 2’. Nevertheless, for in vitro plants of ‘Confenence’, these values were rarely influenced by ASGV and ASPV infection. The result indicated that the changes of CTKs/IAA and IAA/ABA ratios might be responsible for rooting inhibition of in vitro plants of ‘Jinshui no. 2’.

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Abbreviations

6-BA:

6-Benzylamino purine

ABA:

Abscisic acid

ASGV:

Apple stem pitting virus

ASPV:

Apple stem pitting virus

CTK:

Cytokinin

HPLC:

High-performance liquid chromatography

IAA:

Indole-3-acetic acide

IBA:

3-Indole butyric acid

iP:

Isopentenyladenine

iPA:

Isopentenyladenine adenosine

NAA:

1-Naphthaleneacetic acid

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Acknowledgements

This work was financially supported by Chinese Ministry of Agriculture, with the Project nyhyzx-201203076-03, the earmarked fund for Pear Modern Agro-industry Technology Research System CARS-29-10 and the Fundamental Research Funds for the Central Universities with Program No. 2662016PY107. We give our thanks to professor Hong-Ju Hu at Institute of Fruit and Tea, Hubei Academy of Agriculture Sciences, Wuhan, Hubei 430070, China and professor Yuan-Jun Li at Yantai Agricultural Science and Technology Institute, Yantai Academy of Agricultural Science, Yantai, Shandong, China, for kindly providing source pear materials.

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Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China

    Jie Chen & Ni Hong

  2. College of Plant Science and Technology, Hubei Crop Disease Monitoring and Safety Control Key Laboratory, Huazhong Agricultural University, Wuhan, 430070, China

    Jie Chen, Hui-Hui Tang, Liu Li, Shi-Jiao Qin, Guo-Ping Wang & Ni Hong

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  1. Jie Chen
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  2. Hui-Hui Tang
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  3. Liu Li
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  4. Shi-Jiao Qin
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  5. Guo-Ping Wang
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  6. Ni Hong
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Contributions

NH and JC conceived the experiments and wrote the manuscript, JC performed experiments with assistance of S-JQ and LL, H-HT provided the method of root induction, G-PW conceived tissue culture experiments and provided plant materials for this study.

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Correspondence to Ni Hong.

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Communicated by Maurizio Lambardi.

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Chen, J., Tang, HH., Li, L. et al. Effects of virus infection on plant growth, root development and phytohormone levels in in vitro-cultured pear plants. Plant Cell Tiss Organ Cult 131, 359–368 (2017). https://doi.org/10.1007/s11240-017-1289-1

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