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Enhanced production of metabolites by elicitation in Opuntia ficus-indica, Opuntia megacantha, and Opuntia streptacantha callus

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Abstract

Callus from Opuntia streptacantha (cv. Tuna loca), Opuntia megacantha (cv. Rubí reina), and Opuntia ficus-indica (cv. Rojo vigor) were exposed to jasmonic acid (JA) and abiotic stress (drought and UV light) to improve the metabolite production. The callus growth curves, phenolic acids and flavonoids content, antioxidant activity and phenylalanine ammonia lyase (PAL) activity were analyzed under normal and stress conditions. In O. streptacantha callus, the phenolics concentration increased 1.6 to 3 times times in presence of 5% PEG or after irradiation with UV light for 240 min, respectively, while flavonoids triplicate with UV light. A significant increase in antioxidant activity was observed in calli from the three Opuntia species in media with 50 µM JA. The relationships between metabolites/PAL activity, and metabolites/antioxidant activity were analyzed using a surface response methodology. Results showed that PAL activity, induced with PEG and UV, correlated with flavonoids content in O. megacantha and O. ficus-indica calli; PAL activity was related to both flavonoids and phenolics compounds in O. ficus-indica and O. megacantha calli exposed to JA, but only to flavonoids in O. streptacantha callus. In general, the JA stimulated simultaneously the metabolic pathways for phenolics and flavonoids synthesis, while abiotic stress induced mainly flavonoids route. As the stressed Opuntia calli exhibited as high antioxidant activity as cladodes, they are a promising system for research on antioxidant biosynthesis and/or to identify new compounds with antioxidant properties.

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Acknowledgements

The authors are grateful to CONACYT for the Grant No. 142873 (Bilateral Mexico-France Project) and for the fellow no. 247838 to NG Camarena-Rangel.

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Authors and Affiliations

  1. Facultad de Ciencias Químicas, UASLP, Manuel Nava 6, 78210, San Luis Potosí, Mexico

    Nancy Gabriela Camarena-Rangel & María del Socorro Santos-Díaz

  2. Instituto Potosino de Investigación Científica y Tecnológica A.C., Camino a la Presa San José, No. 2055, Lomas 4ª Sección, 78216, San Luis Potosí, Mexico

    Ana Paulina Barba-De la Rosa

  3. Colegio de Postgraduados, Campus Córdova, Km 348 Carretera Federal Córdova-Veracruz, 94946, Amatlán de los Reyes, Veracruz, Mexico

    José Andrés Herrera-Corredor

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  1. Nancy Gabriela Camarena-Rangel
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  2. Ana Paulina Barba-De la Rosa
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  3. José Andrés Herrera-Corredor
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  4. María del Socorro Santos-Díaz
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Correspondence to María del Socorro Santos-Díaz.

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Camarena-Rangel, N.G., Barba-De la Rosa, A.P., Herrera-Corredor, J.A. et al. Enhanced production of metabolites by elicitation in Opuntia ficus-indica, Opuntia megacantha, and Opuntia streptacantha callus. Plant Cell Tiss Organ Cult 129, 289–298 (2017). https://doi.org/10.1007/s11240-017-1177-8

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  • DOI: https://doi.org/10.1007/s11240-017-1177-8

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