Skip to main content
SpringerLink
Log in

Media derived from brown seaweeds Cystoseira myriophylloides and Fucus spiralis for in vitro plant tissue culture

  • Original Article
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

In the present study, the effect of seaweed extract (SE) from Fucus spiralis (Fs), Cystoseira myriophylloides (Cm) and Laminaria digitata (Ld) on in vitro plant tissue culture was examined. Combination of 25 % of SE from Cm with 25 % of MS medium increased adventitious shoot regeneration from Nicotiana benthamiana leaf discs explants by 620 %, when compared to the conventional regeneration medium. Similarly SE from Fs and Ld enhanced regeneration by about 500 %. However, when increasing SE to 50 %, only Cm significantly enhanced shoot regeneration. The effect of SE was also evaluated on in vitro micropropagation of N. benthamiana, grape, plum and apricot by assessing shoot length, number of leaves and internodes. When used alone but at lower concentrations (2.5 and 12.5 %), SE from Fs and Cm resulted in at least the same efficacy as MS alone for micropropagation of N. benthamiana shoots. However, for micropropagation of grapevine, plum and apricot woody plants, a combination of 50 % of SE from Cm or Fs with 50 % of their conventional micropropagation media was necessary. Rooting was also enhanced in N. benthamiana and grapevine, and was correlated with their higher concentrations of indole acetic acid when compared to SE from Ld. This finding, in addition to mineral analysis data, suggests that SE of Fs and Cm contain necessary nutrients and growth regulators to allow their use as medium for in vitro plant culture.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Abbreviations

SE:

Seaweed extracts

Fs:

Fucus spiralis

Cm:

Cystoseira myriophylloides

Ld:

Laminaria digitata

MS:

Murashige and Skoog

SMM:

Shoot multiplication medium

WPM:

Woody plant medium

BAP:

6-Benzylaminopurine

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

NAA:

Naphthalene acetic acid

References

  • Ainsworth EA, Gillespie KM (2007) Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteau reagent. Nat Protoc 2:875–877

    Article  CAS  PubMed  Google Scholar 

  • Aremu AO, Masondo NA, Rengasamy KRR, Amoo SO, Gruz J, Bíba O, Subrtova M, Pencık A, Novak O, Dolezal K, Van Staden J (2015) Physiological role of phenolic biostimulants isolated from Brown seaweed Ecklonia maxima on plant growth and development. Planta 241:1313–1324

    Article  CAS  PubMed  Google Scholar 

  • Banerjee M (2005) In vitro callusing in Stevia rebaodiana Bertoni using cyanobacterial media. Int J Biol 3:163–168

    Google Scholar 

  • Berry WL, Smith OE (1969) Effects of rubidium, potassium and gibberellic acid on plant growth. Plant Cell Physiol 10:161–170

    CAS  Google Scholar 

  • Blunden G (1977) Cytokinin activity of seaweeds extracts. In: Faulkner DL, Fenical WF (eds) Marine natural products chemistry. Plenum, New York, pp 337–343

    Chapter  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein–dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Crouch U, Van Staden J (1991) Evidence for rooting factors in a seaweed concentrate prepared from Ecklonia maxima. J Plant Physiol 137:319–322

    Article  Google Scholar 

  • De Klerk GJ, Guan H, Huisman P, Marinova S (2011) Effects of phenolic compounds on adventitious root formation and oxidative decarboxylation of applied indoleacetic acid in Malus ‘Jork 9’. Plant Growth Regul 63:175–185

    Article  CAS  Google Scholar 

  • Driver JA, Kuniyuki AH (1984). In vitro propagation of Paradox walnut rootstock. Hortiscience 19:507–509

    Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method determination of sugars and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  • Durand N, Briand X, Meyer C (2003) The effect of marine bioactive substances (NPRO) and exogenous cytokinins on nitrate reductase activity in Arabidopsis thaliana. Physiol Plant 119:489–493

    Article  CAS  Google Scholar 

  • Ehmann A (1977) The Van Urk-Salkowski reagent-a sensitive and specific chromogenic reagent for silica gel thin-layer chromatographic detection and identification of indole derivatives. J Chromatogr 132:267–276

    Article  CAS  PubMed  Google Scholar 

  • Featonby-Smith BC, Van Staden J (1983) The effect of seaweed concentrate and fertilizer on the growth of Beta vulgaris. Z Pjlanzenphysiol Bd 112:155–162

    Article  Google Scholar 

  • Gamborg OL (2002) Plant tissue culture biotechnology milestones. In vitro Cell Dev Biol Plant 38:84–92

    Article  Google Scholar 

  • George EF (1993) Plant propagation by tissue culture Part 1: the technology. Excegetics Limited, Westbury, p 574

    Google Scholar 

  • Hanus-Fajerska E, Czura A, Grabski K, Tukaj Z (2009) The effect of conditioned medium obtained from Scenedesmus subspicatus on suspension culture of Silene vulgaris (Caryophyllaceae). Acta Physiol Plant 31:881–887

    Article  Google Scholar 

  • Kalaivanan C, Venkatesalu V (2012) Utilization of seaweed Sargassum myriocystum extracts as a stimulant of seedlings of Vigna mungo (L.) Hepper. Span J Agric Res 10:466–470

    Article  Google Scholar 

  • Khan W, Rayirath UP, Submanian S, Jithesh MN, Rayorath P, Hodges DM, Critchley AT, Craigie JS, Norrie J, Prithivraj B (2009) Seaweed extracts as biostimulants of plant growth and development. Plant Growth Regul 28:386–399

    Article  CAS  Google Scholar 

  • Khan W, Hiltz D, Critchley AT, Prithiviraj B (2011) Bioassay to detect Ascophyllum nodosum extract-induced cytokinin-like activity in Arabidopsis thaliana. J Appl Phycol 23:409–414

    Article  Google Scholar 

  • Kodym A, Zapata Arias FJ (2001) Low cost alternatives for the micropropagation of banana. Plant Cell Tissue Organ Cult 66:67–71

    Article  CAS  Google Scholar 

  • Kowalski B, Jager AK, Van Staden J (1999) The effect of a seaweed on the in vitro growth and acclimatization of potato plantlets. Potato Res 42:131–139

    Article  Google Scholar 

  • Kumar G, Sahoo D (2011) Effect of seaweed liquid extract on growth and yield of Triticum aestivum var. Pusa Gold. J Appl Phycol 23:251–255

    Article  Google Scholar 

  • Latique S, Chernane H, Mansori M, El Kaoua M (2013) Seaweed liquid fertilizer effect on physiological and biochemical parameters of bean plant (Phaesolus vulgaris variety Paulista) under hydroponic system. Eur Sci J 9:174–191

    Google Scholar 

  • Maliro FAM, Lameck G (2004) Potential of cassava flour as a gelling agent in media for plant tissue cultures. Afr J Biotechnol 3:244–247

    Google Scholar 

  • Michael PS (2011) Effects of coconut water on callus initiation and plant regeneration potentials of sweet potato. J Proc Roy Soc New South Wales 144:91–101

    Google Scholar 

  • Mooney PA, Van Staden J (1986) Algae and Cytokinins. J Plant Physiol 12:1

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nelson WR, Van Staden J (1984) The effect of seaweed concentrates on wheat culms. J Plant Physiol 115:433–437

    Article  CAS  PubMed  Google Scholar 

  • Neumann K-H, Kumar A, Imani J (2009) Plant cell and tissue culture-a tool in biotechnology. Springer-Verlag, Berlin

    Google Scholar 

  • Ogero KO, Mburugu GN, Mwangi M, Ombori O, Ngugi M (2012) In vitro micropropagation of cassava through low cost tissue culture. Asian J Agric Sci 4(3):205–209

    Google Scholar 

  • Quoirin M, Lepoivre P (1977) Etude de milieux adaptes aux cultures in vitro de Prunus. Acta Hortic 78:437–442

    Article  Google Scholar 

  • Rayorath P, Jithesh MN, Farid A, Khan W, Palanisamy R, Hankins SD, Critchley AT, Prithiviraj B (2008) Rapid bioassay to evaluate the plant growth promoting activity of Ascophyllum nodosum (L.) Le Jol. using a model plant, Arabidopsis thaliana (L.) Heynh. J Appl Phycol 20:423–429

    Article  CAS  Google Scholar 

  • Reitz SR, Trumble SR (1996) Effects of cytokinins containing seaweed extract on Phaseolus lunatus L.: influence on nutrient availability and apex removal. Bot Mar 39:33–38

    Article  CAS  Google Scholar 

  • Santana MA, Romay G, Matehus J, Vicente-Villardon JL, Demey JR (2009) A simple and low-cost strategy for micropropagation of cassava (Manihot esculenta Crantz). Afr J Biotechnol 8:3789–3897

    Google Scholar 

  • Satish L, Periyasamy R, Arokiam SR, Stanislaus AC, Subramani P, Ramakrishnan R, Manikandan R (2016) Somatic embryogenesis and regeneration using Gracilaria edulis and Padina boergesenii seaweed liquid extracts and genetic fidelity in finger millet (Eleusine coracana). J Appl Phycol 28:2083–2098

    Article  Google Scholar 

  • Seema T, Arnold R, Tiwari A, Mishra RM, Chauhan UK (2011) Cyanobacterial extract and MS media as a novel tool for in vitro regeneration of Stevia rebaudiana Bertoni. J Algal Biomass Utln 2:24–40

    Google Scholar 

  • Seneviratne P, Wijesekara G (1996) The problem of phenolic exudates in in vitro cultures of mature Hevea brasiliensis. J Plant Crops 24:54–62

    Google Scholar 

  • Sharma N, Chauhan RS, Sood H (2015) Seaweed extract as a novel elicitor and medium for mass propagation and picroside-I production in an endangered medicinal herb Picrorhiza kurroa. Plant Cell Tissue Organ Cult 122:57–65

    Article  Google Scholar 

  • Sivanandhan G, Selvaraj N, Ganapathi A, Manickavasagam M (2014) Improved production of withanolides in shoot suspension culture of Withania somnifera (L.) dunal by seaweed extracts. Plant Cell Tissue Organ Cult 119:221–225

    Article  Google Scholar 

  • Sivasankari S, Venkatesalu V, Anantharaj M, Chandrasekaran M (2006) Effect of seaweed extracts on the growth and biochemical constituents of Vigna sinensis. Bioresour Technol 97:1745–1751

    Article  CAS  PubMed  Google Scholar 

  • Stirk WA, Arthur GD, Lourens AF, Novák O, Strnad M, Van Staden J (2004) Changes in cytokinin and auxin concentrations in seaweed concentrates when stored at an elevated temperature. J Appl Phycol 1:31–39

    Article  Google Scholar 

  • Tay SA, Macleod JK, Palni LM, Letham DS (1985) Detection of cytokinins in a seaweed extract. Phytochemistry 24:2611–2614

    Article  CAS  Google Scholar 

  • Thorpe TA, Harry IS (1990) Special problems and prospects in the propagation of woody species. Plant aging. Springer, Berlin, pp 67–74

    Google Scholar 

  • Vinoth S, Gurusaravanan P, Jayabalan N (2012) Effect of seaweed extracts and plant growth regulators on high-frequency in vitro mass propagation of Lycopersicon esculentum L (tomato) through double cotyledonary nodal explant. J Appl Phycol 24:1329–1337

    Article  CAS  Google Scholar 

  • Wiszniewska A, Nowak B, Kołton A, Sitek E, Grabski K, Dziurka M, Długosz-Grochowska O, Dziurka K, Tukaj Z (2016) Rooting response of Prunus domestica L. microshoots in the presence of phytoactive medium supplements. Plant Cell Tissue Organ Cult 125:163–176

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by the Moroccan CNRST (Centre National de Recherche Scientifique et Technique) within the frame work of the RS/2011/22 project.

Author information

Authors and Affiliations

  1. Laboratory of Plant Biotechnology and Ecosystem Valorisation, Faculty of Sciences, University Chouaib Doukkali, El Jadida, Morocco

    Siham Esserti, Mohamed Faize, Lalla Aicha Rifai, Amal Smaili, Malika Belfaiza, Tayeb Koussa & Kacem Makroum

  2. Group of Fruit Tree Biotechnology, Department of Plant Breeding, CEBAS-CSIC, PO Box 164, 30100, Murcia, Spain

    Lydia Faize, Nuria Alburquerque & Lorenzo Burgos

Authors
  1. Siham Esserti
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mohamed Faize
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lalla Aicha Rifai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Amal Smaili
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Malika Belfaiza
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lydia Faize
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Nuria Alburquerque
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Lorenzo Burgos
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Tayeb Koussa
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Kacem Makroum
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mohamed Faize.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Esserti, S., Faize, M., Rifai, L.A. et al. Media derived from brown seaweeds Cystoseira myriophylloides and Fucus spiralis for in vitro plant tissue culture. Plant Cell Tiss Organ Cult 128, 437–446 (2017). https://doi.org/10.1007/s11240-016-1121-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-016-1121-3

Keywords

Search

Navigation