Abstract
The peculiarities of interaction of cyanobacterial photosystem I with redox mediators 2,6-dichlorophenolindophenol (DCPIP) and N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD) were investigated. The higher donor efficiency of the reduced DCPIP form was demonstrated. The oxidized form of DCPIP was shown to be an efficient electron acceptor for terminal iron–sulfur cluster of photosystem I. Likewise methyl viologen, after one-electron reduction, DCPIP transfers an electron to the molecular oxygen. These results were discussed in terms of influence of these interactions on photosystem I reactions with the molecular oxygen and natural electron acceptors.
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Abbreviations
- Asc:
-
Ascorbate
- β-DM:
-
n-dodecyl-β-d-maltoside
- Chl:
-
Chlorophyll
- E m :
-
The midpoint potential
- ET:
-
Electron transfer
- DCPIP:
-
2,6-dichlorophenolindophenol
- DCPIP(H)· :
-
A semi-reduced form of DCPIP
- DCPIPH2 :
-
The fully reduced form of DCPIP
- DCPIPOX :
-
The fully oxidized form of DCPIP
- K m :
-
The apparent Michaelis constant
- MV:
-
Methyl viologen
- Pc:
-
Plastocyanin
- PS I, PS II:
-
Photosystem I, photosystem II
- R MV :
-
The ratio of the rate of oxygen uptake in the presence of MV to the rate of oxygen uptake in the absence of MV
- TMPD:
-
N,N,N′,N′-tetramethyl-p-phenylenediamine
- TMPD·+ :
-
A semi-oxidized form of TMPD
- VO2 :
-
Rate of oxygen uptake in the absence of MV
- VO2 MV :
-
Rate of oxygen uptake in the presence of MV
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Acknowledgements
This work was supported by the Russian Science Foundation (Grant #17-14-01323). In part of higher plant thylakoid membranes (Table 1), the work was supported by the Russian Science Foundation (Grant #14-14-00535). The authors are grateful to Dr. Dmitry Cherepanov for valuable discussion.
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11120_2018_514_MOESM3_ESM.tif
Supplementary Figure 2—The effect of ascorbate concentration of O2 uptake rate (VO2) in the absence (squires, solid line) or presence (triangles, dashed line) of MV (A) and the steady-state P700+ level, ΔP700+, in the presence of MV (B). On A, VO2 values in the presence of 1 mM TMPD are shown for comparison (circles). Trimeric PS I at 2 (A) or 5 (B) µg Chl mL−1, pH 7.6. Data are shown as mean of 3 repetitions ± SE (TIF 104 KB)
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Petrova, A., Mamedov, M., Ivanov, B. et al. Effect of artificial redox mediators on the photoinduced oxygen reduction by photosystem I complexes. Photosynth Res 137, 421–429 (2018). https://doi.org/10.1007/s11120-018-0514-z
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DOI: https://doi.org/10.1007/s11120-018-0514-z