Photosynthesis Research

, Volume 137, Issue 3, pp 421–429 | Cite as

Effect of artificial redox mediators on the photoinduced oxygen reduction by photosystem I complexes

  • Anastasia Petrova
  • Mahir Mamedov
  • Boris Ivanov
  • Alexey Semenov
  • Marina Kozuleva
Original Article


The peculiarities of interaction of cyanobacterial photosystem I with redox mediators 2,6-dichlorophenolindophenol (DCPIP) and N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD) were investigated. The higher donor efficiency of the reduced DCPIP form was demonstrated. The oxidized form of DCPIP was shown to be an efficient electron acceptor for terminal iron–sulfur cluster of photosystem I. Likewise methyl viologen, after one-electron reduction, DCPIP transfers an electron to the molecular oxygen. These results were discussed in terms of influence of these interactions on photosystem I reactions with the molecular oxygen and natural electron acceptors.


Redox mediator Oxygen reduction Photosystem I Methyl viologen 2,6-Dichlorophenolindophenol N,N,N′,N′-tetramethyl-p-phenylenediamine Electron acceptor Midpoint potential 









The midpoint potential


Electron transfer




A semi-reduced form of DCPIP


The fully reduced form of DCPIP


The fully oxidized form of DCPIP


The apparent Michaelis constant


Methyl viologen




Photosystem I, photosystem II


The ratio of the rate of oxygen uptake in the presence of MV to the rate of oxygen uptake in the absence of MV




A semi-oxidized form of TMPD


Rate of oxygen uptake in the absence of MV


Rate of oxygen uptake in the presence of MV



This work was supported by the Russian Science Foundation (Grant #17-14-01323). In part of higher plant thylakoid membranes (Table 1), the work was supported by the Russian Science Foundation (Grant #14-14-00535). The authors are grateful to Dr. Dmitry Cherepanov for valuable discussion.

Supplementary material

11120_2018_514_MOESM1_ESM.docx (13 kb)
Supplementary Table 1 (DOCX 12 KB)
11120_2018_514_MOESM2_ESM.tif (479 kb)
Supplementary Figure 1—The scheme of redox conversations of TMPD and DCPIP (TIF 479 KB)
11120_2018_514_MOESM3_ESM.tif (104 kb)
Supplementary Figure 2—The effect of ascorbate concentration of O2 uptake rate (VO2) in the absence (squires, solid line) or presence (triangles, dashed line) of MV (A) and the steady-state P700+ level, ΔP700+, in the presence of MV (B). On A, VO2 values in the presence of 1 mM TMPD are shown for comparison (circles). Trimeric PS I at 2 (A) or 5 (B) µg Chl mL−1, pH 7.6. Data are shown as mean of 3 repetitions ± SE (TIF 104 KB)


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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.A.N. Belozersky Institute of Physical-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Institute of Basic Biological ProblemsRussian Academy of SciencesPushchinoRussia
  3. 3.Department of Molecular Biology and Ecology of Plants, The George S. Wise Faculty of Life SciencesTel Aviv UniversityTel AvivIsrael

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