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Dissection of Physiological Traits for Terminal Heat Stress Tolerance in Indian Mustard (Brassica juncea L.) Using Microsatellite Markers

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Abstract

Heat stress, especially during the reproductive stage, has been exacerbated by changing climatic conditions, resulting in reduced seed yield in crops like Indian mustard. In the present study, 154 genotypes of Indian mustard were used to find novel SSR marker-based associations for seed yield and physiological traits by linkage disequilibrium. Extensive phenotyping of 154 genotypes for seed yield and various physiological traits was done for two consecutive years (2020–2021 and 2021–2022) in timely and late-sown environments. Out of 237 SSR markers, a total of 111 polymorphic markers containing 312 alleles were used for analysis. Two different models, i.e., general linear model (GLM) and mixed linear model (MLM), were used to detect the significant marker–trait associations for timely and late-sown environment separately. A total of 12 and 10 MTAs were found to be associated under timely and late-sown conditions with seed yield and physiological traits using MLM model. Out of these promising MTAs identified in the present study, five SSRs were specifically detected only in late-sown environments which meant that these specific genomic regions and QTLs were associated under terminal heat conditions. This is the first report on the identification of marker-trait associations for physiological traits under terminal heat stress conditions. It may be possible to develop the molecular markers for significant SSRs after due validation. The findings contribute to our understanding of the genetic basis of heat tolerance and provide valuable information for breeding programs aiming to improve crop productivity under heat stress conditions.

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Acknowledgements

The authors gratefully acknowledge Dr. Naveen Singh (Scientist, IARI, New Delhi) and Dr. P. K. Rai (Principal Scientist and Director, Directorate of Rapeseed and Mustard Research, Bharatpur) for providing the seed material for the research experiment. Authors are also thankful to CSIR (Council of Scientific and Industrial Research)- HRDG, New Delhi, for awarding the Senior Research Fellowship to Raju Ram Choudhary during his doctorate study.

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  1. Raju Ram Choudhary

    Present address: Sri Karan Narendra Agriculture University, Jobner, 303329, Rajasthan, India

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  1. Department of Genetics & Plant Breeding, CCS Haryana Agricultural University, Hisar, 125004, Haryana, India

    Raju Ram Choudhary, Ram Avtar, Pawan Kumar, Manjeet Singh, Mahavir Bishnoi, Neeraj Kumar & Rakesh Punia

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Contributions

RRC: conducted the research, recorded data, and drafted the manuscript; RA: supervision, guidance, planning of experiment, and manuscript editing; PK: statistical analysis, data interpretation, and manuscript editing; MS: assisted in wet lab work; MB: manuscript editing; NK: crop management and technical help; RP: crop management and data recording.

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Correspondence to Pawan Kumar.

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Choudhary, R.R., Avtar, R., Kumar, P. et al. Dissection of Physiological Traits for Terminal Heat Stress Tolerance in Indian Mustard (Brassica juncea L.) Using Microsatellite Markers. Plant Mol Biol Rep (2024). https://doi.org/10.1007/s11105-024-01456-3

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