Abstract
Aims
Encroachment of woody species into grasslands is a global phenomenon that affects ecosystem services, including soil organic carbon (SOC) storage and microbial community structure. We determined stocks of SOC and soil microbial biomass as affected by conversion of grasslands to coniferous forests.
Methods
We examined SOC and soil δ13C signatures under three vegetation covers: native grasslands, eastern redcedar (Juniperus virginiana), and ponderosa pine (Pinus ponderosa), at six soil depths (0 − 10, 10 − 30, 30 − 100, 100 − 170, 170 − 240, 240 − 300 cm). Fatty acid methyl esters (FAMEs) were used to quantify soil microbial biomass (MB) and as biomarkers for bacteria, arbuscular mycorrhizal fungi (AMF), and saprophytic fungi (SF) which includes ectomycorrhizal fungi (ECM).
Results
Native grasslands had higher overall SOC and MB stocks in the top 300 cm soil depth (7.39 kg SOC m− 2 and 65.6 mmol MB m− 2) than converted cedar (6.80 kg SOC m− 2 and 38.4 mmol MB m− 2) or pine (5.87 kg SOC m− 2 and 39.9 mmol MB m− 2) forest. Losses of AMF biomass occurred on conversion of native grasslands to pine forest. Stocks of SF biomass only differed with vegetation type in topsoil.
Conclusions
Conversion of native grasslands to cedar or pine forest increased topsoil (0 − 10 cm) SOC storage due to needle litter accumulation. Conversion of native grasslands to forests decreased subsoil (10 − 300 cm) SOC stocks and had replaced, by increasing depth, 30 to 19% and 45 to 26% of grassland C with cedar and pine C, respectively. Changes in soil pH, nutrients, and soil microbial communities accelerated SOC turnover.
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Introduction
Invasive species threaten biodiversity and alter ecosystem functions worldwide (Gurevitch and Padilla 2004). Globally, substantial proportions of grasslands were lost to the encroachment of woody species in the past decades (Arasumani et al. 2021; Bora et al. 2021; Hynson et al. 2013; van Mantgem et al. 2021). These alterations can reduce grasslands ecosystem resilience and resistance and increase susceptibility to climate change, fire, and further encroachment (Arasumani et al. 2021; Bora et al. 2021; Lubetkin et al. 2017). Conversion of native grasslands to wooded areas alters microclimate and reduces light intensity and temperature for understory communities, and thus impacts grass cover and productivity (Bora et al. 2021; Chikowore et al. 2021; Mazis et al. 2021). Encroachment also affects soil hydrological regime, due to increased evapotranspiration and decreased soil water availability relative to grasslands (Awada et al. 2013; Curtis et al. 2019; Kormos et al. 2017). Also, coniferous species can decrease soil pH and bulk density and alter nutrient availability and soil organic carbon (SOC) storage (Hou et al. 2019; Podwika et al. 2020).
Encroachment of coniferous species into native grasslands increases aboveground plant biomass (Hoeksema et al. 2020) and has been proposed as a potential strategy to increase litter input to soil (Lugato et al. 2021), thereby directly increasing SOC storage in topsoil (i.e., A horizon). However, much less is known about the effects of conifer encroachment on subsoil (i.e., below A horizon) SOC storage and turnover (Nuñez et al. 2021). Although subsoils are usually considered as the limited access/low activity region for microbes (Kleber and Lindsley 2022), they are highly relevant for SOC storage, considering that 77% of SOC is estimated to be stored in the depth of 30 − 300 cm globally (Lal 2018). Encroachment of coniferous species and associated changes in the quantity, quality, and distribution of C input, rooting depth, and soil microbial community, can either decrease or increase SOC storage and turnover (Hoeksema et al. 2020; Nuñez et al. 2021). Increased input of new C can stimulate losses of old SOC stored in deep soil (Fontaine et al. 2007) or lead to net increases in SOC due to compensation (Jilkova et al. 2019; Liang et al. 2018). Additionally, conversation of native grasslands to wooded areas can alter soil microbial communities and therefore SOC turnover. Encroachment of eastern redcedar into grasslands is facilitated by forming mutualisms with native grassland arbuscular mycorrhizal fungi (AMF) that shifts over time to AMF species that are better adapted to changes in soil edaphic properties as tree density increases (Liang et al. 2017). Pine species encroachment frequently results in co-invasion of ectomycorrhizal fungi (ECM), of which pines are obligate mutualists (Hynson et al. 2013; Moyano et al. 2019). Conversion of grasslands to pine plantations could shift the dominance from AMF to ECM (Hoeksema et al. 2020; Liang et al. 2017), thereby accelerating SOC decomposition since ECM are generally more capable of decomposing SOC compared to AMF (Hoeksema et al. 2020; Zak et al. 2019).
To study SOC turnover and its mean residence time (MRT), δ13C signatures can be used as a tool to examine the contribution of different sources to SOC. In the scenario of C4–C3 vegetation change, the SOC derived from the original C4 plants and new C3 plants can be differentiated based on plant δ13C signature. The uptake of 13 C by C4 and C3 plants via photosynthesis are 4‰ and 19‰ less than the 13 C in the atmosphere, respectively (Zacháry 2019). Consequently, C4 plant litter is more enriched in 13 C compared to C3 plant litter. Isotope signatures also stratify by soil depth. Surface soil δ13C signatures are generally consistent with current vegetation cover, whereas deep soil δ13C signatures usually reflect previous vegetation cover (Gautam et al. 2017).
Grasslands are one of the most threatened and least protected ecosystems globally, with few intact grassland ecosystems remaining (Scholtz and Twidwell 2022). The semi-arid, C4 dominated grasslands of the Nebraska Sandhills remain one of the relatively intact temperate grasslands in the world (Jacobson et al. 2019; Scholtz and Twidwell 2022). Invasion or spread of coniferous species into this grassland ecosystem may strongly affect SOC storage and turnover, but studies evaluating the long-term consequences of this on subsoil SOC below 30 cm are lacking. To address this knowledge gap, we measured SOC, soil 13 C natural abundance, soil physical and chemical properties, and soil microbial biomass using fatty acid methyl esters (FAMEs) to a soil depth of 300 cm under a native grassland dominated by C4 grasses as the benchmark, and under two C3 forests planted on native grassland. Our goal was to assess whether conversion of native grasslands to coniferous forests decreased SOC stocks and the changes in SOC storage that could, in part, be explained by changes in soil microbial biomass and microbial community structure.
Materials and methods
Site selection, experiment design, and soil sampling
Study site was located at the Nebraska National Forest (NNF) at Halsey (865 m elevation; 41°51′45″ N, 100°22′06″ W), a 25,000-ha experimental forest established in the Sandhills grasslands in 1902. It is currently the largest man-made forest in the United States (Adane and Gates 2015). The forest was hand-planted with various coniferous species, including eastern redcedar (Juniperus virginiana) and ponderosa pine (Pinus ponderosa), in the 1930s. Hereafter and throughout the manuscript, eastern redcedar and ponderosa pine are respectively referred as cedar and pine for simplicity. The climate is semiarid continental (Singh et al. 2021), with a mean annual temperature of 8.5 °C and mean annual precipitation of 570 mm. The soils are loose, well-drained Valentine (Mixed, Mesic Typic Ustipsamments), containing greater than 90% of sand. See soil texture in Table S1. The texturally uniform soils, similar plantation ages, well-recorded site history, and the presence of native grasslands in the area provide an ideal proxy for studying the effects of conifer encroachment into native grassland on SOC.
Two forested (pine and cedar) and one native grassland site were selected at NNF for the study. The grasslands are dominated by perennial C4 grass communities, including sand bluestem (Andropogon hallii), prairie sandreed (Calamovilfa longifolia), sand dropseed (Sporobolus cryptandrus). Photosynthesis of the C4 C fixation pathway discriminates less against the 13 C isotope in CO2, compared to the C3 C fixation pathway of cedar and pine, so grassland litter is less depleted in 13 C than cedar or pine litter. The grassland SOC therefore contains a δ13C signature, from which cedar- or pine-derived C can be differentiated. The stand age, average tree density, and height for cedar and pine were 65 and 82 years, 356 and 375 tree ha− 1, and 9 and 17 m, respectively (Mazis et al. 2021; Mellor et al. 2013). Pine and cedar stands contain understory vegetation of grasses, forbs, shrubs, and cactus (Mazis et al. 2021). Soil samples were collected in June 2021. Three transects were established within each vegetation type. The transects were 40 m long and ~ 40 m apart from each other to comply with permitting restrictions. Four soil cores were sampled along each transect and the sampling locations were ~ 13 m apart from each other. Soil cores were taken using a hydraulic auger (Conservation and Survey Division, University of Nebraska-Lincoln) to the soil depth of 300 cm. Soil cores were shipped in coolers with ice and then stored in freezer before processing. Each soil core was processed and analyzed individually. The intact soil cores were divided into three genetic horizons: A, AC, and C, approximately corresponding to the depth increments of 0 − 10 cm, 10 − 30 cm, and 30 − 300 cm, respectively. The C horizon was further divided into four increments: 30 − 100, 100 − 170, 170 − 240, and 240 − 300 cm, resulting in six depth increments in total. See specific sampling depth for each sample in the archived data (https://github.com/lilidong1111/SOC). Overall, our experiment had two factors: vegetation type and soil depth. Each combination of factors had n = 12: three replicates (i.e., transects), and each replicate had four subsamples (i.e., cores per transect) for a total of 216 soil samples. Due to small amount of soil for some surface horizons, n ≤ 12 for some analyses. The litter layer consisted of decomposed and partially decomposed plant materials, so the litter layer was sampled separately from the soils. We collected the plant materials that did not pass through a 2 mm sieve in the litter layer.
Laboratory analyses
The C concentrations and δ13C values of litter and soil were analyzed at the Water Sciences Laboratory in the Nebraska Water Center according to Mellor et al. (2013). Litter was washed with deionized water. Litter and soil were air-dried and ground to pass a 200 μm sieve for C determination by dry combustion using an Elemental Analyzer (Carlo Erba NA 1500; Milan, Italy). When soil pH > 7.2, soil samples were acid-washed to remove the inorganic carbon before SOC measurement. Litter and soil 13 C/12 C values were determined using an Elemental Analyzer coupled with an Isotope Ratio Mass Spectrometer (GV Isochrom; Manchester, UK). The percentage of the original grassland SOC replaced by tree litter C under the cedar and pine stands (Creplaced) was calculated using the δ13C value of the grasslands soil (δ13Cgrassland), the cedar and pine soils (δ13Cforest), and the cedar and pine litter (δ13Clitter = − 27.8‰ and − 27.2‰, respectively): Creplaced = (δ13Cforest − δ13Cgrassland)/( δ13Clitter − δ13Cgrassland)×100% (Zacháry 2019). The MRT of grasslands SOC in the cedar and pine soils was calculated by the decay rate constant (k) and the time since vegetation change (t): MRT = 1/k = − t/ln (1 − Creplaced) (Zacháry 2019). The calculation of MRT was based on several assumptions (Mellor et al. 2013), so we consider these MRT values as a rough estimation rather than a precise dating approach. The assumptions were that (1) SOC decomposition followed first-order kinetics, (2) C3 and C4 derived SOC decomposed at the same rate, and (3) 13 C concentrations in different plant components were the same. Although these assumptions may be not met, our study was based on two tree species with similar δ13C signatures planted at approximately the same time, which allowed us to conduct a comparative evaluation between the contributions of the two tree species to SOC turnover.
Microbial fatty acid methyl esters (FAMEs) were extracted from 10 g air-dried soil using 0.2 M KOH in methanol (Grigera et al. 2007). Details are described in supplementary material. Thirty of the most dominant FAMEs found in microorganisms were used as a proxy for soil microbial biomass (nmol FAMEs g− 1 soil), sixteen FAMEs were used to represent bacterial biomass and community structure (Bååth and Anderson 2003), C18:2cis 9,12 was used to represent SF biomass, including ECM biomass (Bååth and Anderson 2003), and C16:1cis11 was used to represent AMF biomass (Olsson 1999).
Bulk density was calculated by dividing dry soil mass by soil core volume. Soil texture was determined by the hydrometer method (Bouyoucos 1951) and the ultrasonication method (Greenberg et al. 2019; Remus et al. 2018). Soil pH was measured using the 1:1 soil to water slurry method. Nitrate-N was measured by the cadmium reduction method using potassium chloride as extraction agent (Gelderman and Beegle 1998). Soil P was measured by the Olsen method (Frank et al. 1998). Soil K, Ca, and Mg were measured using ammonium acetate (Warncke and Brown 1998). Soil S was measured using monocalcium phosphate by the turbidimetric procedure (Combs et al. 1998). Soil Zn, Fe, and Mn were measured using diethylenetriaminepentaacetic acid (Whitney 1998).
The equivalent soil mass method
The equivalent soil mass (ESM) method was used to account for significant differences in soil masses due to impacts of land use change on bulk density (Poeplau and Don 2013; von Haden et al. 2020; Wendt and Hauser 2013). Fixed depth concentrations of SOC and plant-available nutrients were transformed to fixed mass stocks using a cubic spline interpolation method developed by von Haden et al. (2020) (RStudio-2022.12.0-353.dmg, PBC, Boston, MA, USA). Grassland soil masses were used as the references for pine and cedar soils in our calculations. The reported stocks in our study are based on ESM. Even though the stocks are presented by sampled depth, they are actually based on the ESM depth rather than the sampled depth; Therefore, the ESM stocks do not necessarily pertain to the sampled depth, but instead they are the estimated values that would have been obtained if the soils were sampled according to the reference soil mass rather than the sampled depth (von Haden et al. 2020).
Statistical analyses
A two-way mixed model ANOVA was used to determine the main and interaction effects of vegetation type and soil depth on SOC and the other soil properties (Glimmix procedure; SAS 9.4, SAS Institute Inc., Cary, NC, USA). Vegetation type and soil depth were the fixed factors, with soil depths as repeated measures, and transect and soil core were the random factors. Least Squares Means were compared by Fisher’s Least Significant Difference. Data were log-transformed to achieve normal distribution when necessary. The univariate procedure was used for checking normality of residuals. Normality was determined by the Shapiro-Wilk’s test. Equal variance was determined by the Levene’s test. Significance was set at a 0.05 level. Results were reported as untransformed mean ± arithmetic standard error. Treatment effects on 14 bacterial FAMEs (nmol%) by depth interval to 100 cm were explored using canonical discriminant analysis (CDA) to identify changes in bacterial community structure (CANDISC procedure; SAS 9.4, SAS Institute Inc., Cary, NC, USA). Two bacterial FAMEs, a10MeC18:0 and cyC19:11,12, were removed for CDA due to detection of loss at depth.
Results
Stocks of SOC
Vegetation type and soil depth had an interaction effect on SOC stock (p < 0.01, Fig. 1). In the 0 − 10 cm depth, grasslands had lower SOC stock than cedar and pine forests (0.91 ± 0.09 kg m− 2vs. 1.53 ± 0.16 and 1.50 ± 0.15 kg m− 2, p = 0.047). In the 10 − 240 cm depths, grasslands had higher SOC stock than cedar and pine forests. In the 240 − 300 cm depth, SOC stocks were not significantly different under the three vegetation types (p = 0.20). Throughout the 0 − 300 cm depth, grassland had the highest overall SOC stock, 88 ± 1% of which was in the 10 − 300 cm depth (Tables 1 and 2).
Stocks of SOC based on equivalent soil mass (ESM) at different soil depths under three vegetation cover types: cedar (Juniperus virginiana), pine (Pinus ponderosa), and native semi-arid C4 grasslands. Black bars represent standard errors (n = 12). Same letters indicate no significant differences among vegetation cover types within each soil depth at the 0.05 level
Soil δ13C values and mean residence time (MRT)
Vegetation type and soil depth had an interaction effect on soil δ13C values (p < 0.01, Fig. 2). In the 0 − 10 cm depths, grassland soil had significantly less depleted δ13C values than cedar and pine forest soils. This trend was consistent with that in the litter layer (Table S2). In the 10 − 100 cm depths, grassland soil still had less depleted δ13C values than cedar and pine forest soils. In the 100 − 170 cm depth, grassland soil had less depleted δ13C values than pine forest soil but more depleted than cedar forest soil. In the 170 − 240 cm depth, soil δ13C values were not different under these three vegetation types. In the 240 − 300 cm depth, grassland soil had more depleted δ13C values than cedar and pine forest soils. Soil δ13C values significantly increased in the 10 − 300 cm depth compared to the 0 − 10 cm depth regardless of vegetation type. The MRT of the original grassland SOC under cedar and pine stands was lower in the 0 − 10 cm depth (73 − 149 years) than that in the 10 − 300 cm depths (158 − 352 years, Table 3). The MRT was lower under pine (73 − 306 years) than that under cedar (149 − 352 years).
Soil δ13C values at different soil depths under three vegetation cover types: cedar (Juniperus virginiana), pine (Pinus ponderosa), and native semi-arid C4 grasslands. Black bars represent standard errors (n = 12). Same letters indicate no significant differences among vegetation cover types within each soil depth at the 0.05 level
Stocks of soil microbial biomass
Grassland had the highest overall microbial biomass stock to 300 cm (65.7 ± 11 mmol FAMEs m− 2) followed by similar stocks in pine (39.9 ± 10 mmol FAMEs m− 2) and cedar (38.9 ± 4 mmol FAMEs m− 2) (Table 4). The percent distribution of microbial biomass by depth interval was similar among vegetation types in surface soil but declined under cedar at depths below 30 cm (Table 4). In contrast, the combined biomass of SF and ECM varied by vegetation type across depth intervals except in surface soil where grassland ≥ cedar ≥ pine (Fig. 3D). The CDA analysis of bacterial FAMEs to 100 cm resulted in six significant canonical functions with the first two accounting for 62% of the variation in soil bacterial communities. All nine treatment combinations were different from each other (p < 0.0001).
Fatty acid methyl ester (FAME) stocks at different soil depths under three vegetation cover types: cedar (Juniperus virginiana), pine (Pinus ponderosa), and native semi-arid C4 grasslands. AMF, arbuscular mycorrhizal fungi; SF, saprophytic fungi; ECM, ectomycorrhizal fungi. Black bars represent standard errors (n = 12). Same letters indicate no significant differences among vegetation cover types within each soil depth at the 0.05 level
Discussion
Topsoil SOC storage increases on conversion of native grassland to cedar or pine forest
In topsoil (0 − 10 cm), the conversion of native grassland to cedar and pine forest increased SOC storage by 69 ± 7% and 65 ± 6%, respectively (Fig. 1). Cedar and pine produce larger amounts of aboveground biomass and therefore input more litter to the topsoil than grass. Indeed, the topsoil δ13C values under cedar, pine and grass reflected the δ13C signatures in the plant litter (Fig. 2 and Table S2), indicating significant input of C from plant litter to topsoil. Cedar- and pine-derived C contributed to 40 ± 4% and 70 ± 4% of the SOC storage in the topsoil (Table 3). The greater accumulation of pine litter in the topsoil may result from higher tree density and height compared to cedar, as well as higher C concentration of pine litter (Table S2). The higher C concentration of pine litter can decelerate decomposition (Craig et al. 2018; Fernandez et al. 2020; Lin et al. 2017).
Subsoil SOC storage declines on conversion of native grassland to cedar or pine forest
Cedar and pine have lower belowground allocation of C than grass
Despite more litter input to the topsoil (0 − 10 cm), cedar or pine forests did not increase SOC storage in the subsoil (10 − 300 cm) compared to grasslands (Fig. 1). Grasslands contained 26 ± 3% and 50 ± 6% more SOC than cedar and pine forests in the subsoil (Table 2), in part because of higher allocation of C belowground (February et al. 2020; Jackson et al. 2002; Wigley et al. 2020) and greater abundance of fine roots with higher root tip attrition and faster turnover (Upson et al. 2016). The SOC losses will continue until the new C input from cedar and pine can accumulate in the subsoils and compensate for the losses of the original SOC, maybe over several centuries or even millennia. The new C input to the topsoil can move to the subsoils through bioturbation and downward leaching and might accumulate over time (Cagnarini et al. 2019; Vormstein et al. 2020). The accumulation rate of the C input from new plants can increase with stand age as the roots and rhizome network continue to establish and develop, providing more C input (Leifeld et al. 2021; Richards et al. 2017). The evaluations of the effects of cedar and pine invasion into native grassland on C storage need to be continued for long-term.
Changes in subsoil SOC storage mirror soil microbial biomass
Greater subsoil SOC storage under grasslands may be also associated with the higher microbial biomass (Fig. 3A). This may be fostered by greater herbaceous plant diversity and productivity in native grasslands compared to cedar and pine forests at this field site (Mazis et al. 2021). Higher plant diversity and productivity may favor faster microbial growth and turnover leading to greater production of microbial products and necromass (Prommer et al. 2020) and ultimately SOC formation (Camenzind et al. 2023). However, accumulation of SOC and associated microbial biomass depend on stabilization mechanisms. The higher clay and silt contents under grassland, especially in the subsoil (Tables S1), may facilitate SOC accumulation through clay-associated microbial necromass and products (Churchman et al. 2020; Georgiou et al. 2022; Matus 2021). Clay and clay lenses can retain more water and nutrients (Pepper and Gentry 2015), providing better habitats for soil microbes. Overall, the greater soil moisture (Mazis et al. 2021), pH, and nutrients under grassland (Table 1) would be more favorable to microbial processes central to SOC formation and stabilization.
Conversion of native grassland to cedar or pine forest leads to changes in soil microbial communities
Besides microbial biomass, changes in microbial community structure might also lead to SOC losses under cedar and pine forests. We observed a shift in soil microbial community composition from AMF and bacteria to SF and ECM when converting native grassland to cedar or pine forests (Fig. 3). Although SF and EMC did not differ in amount with vegetation (Fig. 3D), losses of bacterial and AMF biomass under cedar and pine (Fig. 3B and C) make SF and ECM a larger proportion of the total microbial biomass, especially under pine. This shift in microbial community composition is likely to alter microbial community function. Many SF and ECM are capable of decomposing SOC to obtain nutrients such as N (Pellitier et al. 2021; Shah et al. 2016; Zak et al. 2019). Soil nutrients are likely limited in our experimental sites as reflected by the low nutrient and high sand contents (Table 1 and S1) and no anthropogenic input. Thus, decomposition may be highly dependent on nutrient scavenging in these low nutrient soils. Despite the losses of AMF in both cedar and pine soils, cedar supports a higher biomass of AMF compared to pine (Fig. 3C). AMF can allocate 4 − 25% of photosynthetic C from their plant host to fungal mycelia (Parihar et al. 2020) that contributes to the pool of microbial products and necromass (Agnihotri et al. 2022; Jörgensen et al. 2022). Although soil bacteria carry out many of the same functions as SF, their biomass and activities are highly dependent on microsite conditions given the small pores they tend to inhabit. Evidence for a change in soil properties on conversion of grassland to cedar or pine forest is clearly shown for the 0 − 100 cm depth by a shift in bacterial community structure (Fig. 4).
SOC turnover rate and storage by soil depth
The original grassland SOC had shorter mean residence time (MRT) in topsoil (0 − 10 cm) than subsoil (10 − 300 cm, Table 3), suggesting more stabilized SOC in the subsoil. Usually, SOC stabilized in subsoil is horizontally stratified and its MRT increases with soil depths (Rumpel and Kögel-Knabner 2011). However, our MRT from 10 to 300 cm showed no significant differences (Table 3). This unexpected homogeneity of MRT in the subsoil might result from the weakly developed soil structure with little horizonation and a more homogenous distribution of decomposition-relevant resources such as water and nutrients across the soil profile. Since the majority of the SOC in the 0 − 300 cm depth (74 ± 5% to 88 ± 1%) was stored in the subsoil (Table 2), focusing solely on topsoil can lead to misinterpretations. Subsoils may play a more critical role in terrestrial C budget than previously assumed.
Conclusions
We evaluated the SOC storage and turnover as affected by vegetation change from native semi-arid C4 dominated grasslands to eastern red cedar or ponderosa pine forest. Conversion of native grasslands to cedar or pine forest increased topsoil SOC storage but decreased subsoil SOC storage. The decrease in SOC storage was associated with reduced microbial biomass and altered microbial community structure under forests relative to the grasslands. This decrease may also be mediated by the declines in clay content, soil moisture, pH, and nutrients. Our results demonstrated the critical role of subsoil in SOC storage. Subsoil should be considered for more accurate evaluation of global SOC storage. In the scenario of vegetation cover change from native grasslands to cedar or pine forest, the potential aboveground C storage in woody biomass might partially offset the belowground C loss, which needs further investigation.
Data Availability
Data are archived at GitHub (https://github.com/lilidong1111/SOC).
Abbreviations
- SOC:
-
Soil organic carbon
- MRT:
-
Mean residence time
- MB:
-
Microbial biomass
- FAME:
-
Fatty acid methyl ester
- AMF:
-
Arbuscular mycorrhizal fungi
- SF:
-
Saprophytic fungi
- ECM:
-
Ectomycorrhizal fungi
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Acknowledgements
This research was supported by the McIntire Stennis Forestry Funds, U.S. Department of Agriculture (USDA), accession Number 1017953. The authors wish to acknowledge the Nebraska National Forest for the access to the field site, and Conservation and Survey Division, UNL for soil sampling.
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Li, L., Hosseiniaghdam, E., Drijber, R. et al. Conversion of native grassland to coniferous forests decreased stocks of soil organic carbon and microbial biomass. Plant Soil 491, 591–604 (2023). https://doi.org/10.1007/s11104-023-06138-9
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DOI: https://doi.org/10.1007/s11104-023-06138-9