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Interactive effects of nitrogen and water addition on soil microbial resource limitation in a temperate desert shrubland

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Abstract

Aims

Soil microorganisms play critical roles in regulating soil biogeochemical cycles, but their growth and metabolic activities are usually limited by resource availability, especially in water-limited desert ecosystems under global change. However, there is little information on the interactions of nitrogen (N) enrichment and increased precipitation on soil microbial resource limitation in desert-shrubland ecosystems.

Methods

This study examined the effects of long-term (9 years) N and water (W) additions (i.e., 5 g N m−2 year−1 and 30% ambient precipitation increase, and their combination) on the activities of soil extracellular enzymes (EEAs) and soil microbial resource limitation in the Gurbantunggut Desert in northwestern China.

Results

N and W addition significantly enhanced soil microbial carbon (C) and phosphorus (P)-acquiring enzymes, and significantly induced microbial growth from N to P limitation in the desert ecosystem. Furthermore, W addition and simultaneous addition of N and W alleviated C limitation; however, N addition alone increased the microbial C limitation. Thus, the interaction alleviated the negative impact of N addition on soil microbial C limitation, but increased the microbial P limitation. Soil microbial C limitation was primarily driven by the soil available N/P ratio, whereas the soil microbial P limitation was primarily controlled by soil total N/P ratio.

Conclusions

The influence of either N or W addition on the biogeochemical processes in desert ecosystems can be altered by their concurrent addition. Overall, our findings showed that increased precipitation was more effective than N accumulation in modifying microbial metabolism in desert ecosystems, and this effect was co-regulated by the relative contents of N and P in the soil. These findings can aid in the prediction of terrestrial C and nutrient flows under global change.

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Funding

This study was supported by the National Natural Science Foundation of China (41730638), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA23070201), and the Funding of Special Support Plan of Young Talents Project of Shaanxi Province and National Forestry and Grassland Administration in China (20201326015).

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Authors and Affiliations

  1. State Key Laboratory of Soil Erosion and Dryland Farming On the Loess Plateau, Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, No. 26 Xinong Road, Yangling, 712100, Shaanxi, China

    Jiwei Li, Yu Zhang, Zhouping Shangguan & Lei Deng

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Jiwei Li, Yu Zhang, Zhouping Shangguan & Lei Deng

  3. State Key Lab of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, South Beijing Road, Urumqi, 830011, Xinjiang, China

    Jiangbo Xie

  4. State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin’an, 311300, China

    Jiangbo Xie

  5. Institute of Soil and Water Conservation, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Jiwei Li, Lingbo Dong, Zhouping Shangguan & Lei Deng

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  1. Jiwei Li
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  2. Jiangbo Xie
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  3. Yu Zhang
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Correspondence to Lei Deng.

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Li, J., Xie, J., Zhang, Y. et al. Interactive effects of nitrogen and water addition on soil microbial resource limitation in a temperate desert shrubland. Plant Soil 475, 361–378 (2022). https://doi.org/10.1007/s11104-022-05371-y

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