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The iron-chelate transporter OsYSL9 plays a role in iron distribution in developing rice grains

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Rice OsYSL9 is a novel transporter for Fe(II)-nicotianamine and Fe(III)-deoxymugineic acid that is responsible for internal iron transport, especially from endosperm to embryo in developing seeds.

Abstract

Metal chelators are essential for safe and efficient metal translocation in plants. Graminaceous plants utilize specific ferric iron chelators, mugineic acid family phytosiderophores, to take up sparingly soluble iron from the soil. Yellow Stripe 1-Like (YSL) family transporters are responsible for transport of metal-phytosiderophores and structurally similar metal-nicotianamine complexes. Among the rice YSL family members (OsYSL) whose functions have not yet been clarified, OsYSL9 belongs to an uncharacterized subgroup containing highly conserved homologs in graminaceous species. In the present report, we showed that OsYSL9 localizes mainly to the plasma membrane and transports both iron(II)-nicotianamine and iron(III)-deoxymugineic acid into the cell. Expression of OsYSL9 was induced in the roots but repressed in the nonjuvenile leaves in response to iron deficiency. In iron-deficient roots, OsYSL9 was induced in the vascular cylinder but not in epidermal cells. Although OsYSL9-knockdown plants did not show a growth defect under iron-sufficient conditions, these plants were more sensitive to iron deficiency in the nonjuvenile stage compared with non-transgenic plants. At the grain-filling stage, OsYSL9 expression was strongly and transiently induced in the scutellum of the embryo and in endosperm cells surrounding the embryo. The iron concentration was decreased in embryos of OsYSL9-knockdown plants but was increased in residual parts of brown seeds. These results suggested that OsYSL9 is involved in iron translocation within plant parts and particularly iron translocation from endosperm to embryo in developing seeds.

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Acknowledgements

We thank Dr. Nicolaus von Wirén (Leibniz Institute of Plant Genetics and Crop Plant Research) for kindly providing plasmids pDR195 and pDR196-ZmYS1, Dr. David J. Eide (Department of Nutritional Sciences, University of Wisconsin-Madison) for kindly providing yeast strain fet3fet4, Dr. Hiroyuki Watanabe (Hasegawa Kouryou) for kindly providing chemically synthesized NA, and Dr. Kosuke Namba (Tokushima University) for kindly providing chemically synthesized DMA. We also thank Ms. Hiroko Hori (Ishikawa Prefectural University) for assistance with the rice culture and analysis. This research was supported by the Japan Science and Technology Agency program ALCA (to N.K.N.), and JSPS KAKENHI Grant Number 16H04891 (to N.K.N.).

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  1. Takeshi Senoura

    Present address: KUBIX Inc., 3-570 Suematsu, Nonoichi, Ishikawa, 921-8836, Japan

  2. Michiko Takahashi

    Present address: Faculty of Agriculture, Utsunomiya University, 350 Mine-machi, Utsunomioya, Tochigi, 321–8505, Japan

Authors and Affiliations

  1. Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi, Ishikawa, 921-8836, Japan

    Takeshi Senoura, Emi Sakashita, Takanori Kobayashi, May Sann Aung, Hiroshi Masuda & Naoko K. Nishizawa

  2. Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Michiko Takahashi, Hiromi Nakanishi & Naoko K. Nishizawa

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  1. Takeshi Senoura
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  2. Emi Sakashita
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Contributions

NKN and HN designed the research. TS, ES and TK performed the experiments with assistance from MT, MSA and HM. TS and ES analyzed the data. TS and TK wrote the paper.

Corresponding author

Correspondence to Naoko K. Nishizawa.

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Senoura, T., Sakashita, E., Kobayashi, T. et al. The iron-chelate transporter OsYSL9 plays a role in iron distribution in developing rice grains. Plant Mol Biol 95, 375–387 (2017). https://doi.org/10.1007/s11103-017-0656-y

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