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PsbP-induced protein conformational changes around Cl− ions in the water oxidizing center of photosystem II

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Photosynthetica

Abstract

PsbP is an extrinsic protein of PSII having a function of Ca2+ and Cl− retention in the water-oxidizing center (WOC). In order to understand the mechanism how PsbP regulates the Cl− binding in WOC, we examined the effect of PsbP depletion on the protein structures around the Cl− sites using Fourier transform infrared (FTIR) spectroscopy. Light-induced FTIR difference spectra upon the S1→S2 transition were obtained using Cl−-bound and NO3−-substituted PSII membranes in the presence and absence of PsbP. A clear difference in the amide I band changes by PsbP depletion was observed between Cl−-bound and NO3−-substituted PSII samples, indicating that PsbP binding perturbed the protein conformations around the Cl−ion(s) in WOC. It is suggested that PsbP stabilizes the Cl− binding by regulating the dissociation constant of Cl− and/or an energy barrier of Cl− dissociation through protein conformational changes around the Cl− ion(s).

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Abbreviations

DCMU:

3-(3,4-dichlorophenyl)-1,1-dimethylurea

FTIR:

Fourier transform infrared

Mes:

2-(N-morpholino) ethanesulfonic acid

PMS:

phenazine methosulfate

WOC:

water-oxidizing center

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Authors and Affiliations

  1. Division of Material Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan

    J. Kondo & T. Noguchi

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  1. J. Kondo
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  2. T. Noguchi
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Correspondence to T. Noguchi.

Additional information

Acknowledgments: This study was supported by the Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (17H03662 to T.N.).

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Kondo, J., Noguchi, T. PsbP-induced protein conformational changes around Cl− ions in the water oxidizing center of photosystem II. Photosynthetica 56, 178–184 (2018). https://doi.org/10.1007/s11099-017-0749-5

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  • DOI: https://doi.org/10.1007/s11099-017-0749-5

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