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Effects of High-Fat Diet on Neuronal Damage, Gliosis, Inflammatory Process and Oxidative Stress in the Hippocampus Induced by Transient Cerebral Ischemia

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Abstract

In this study, we investigated the effects of a normal diet (ND) and high-fat diet (HFD) on delayed neuronal death in the gerbil hippocampal CA1 region after transient cerebral ischemia. In the HFD-fed gerbils, ischemia-induced hyperactivity was significantly increased and neuronal damage was represented more severely compared to the ND-fed gerbils. Ischemia-induced glial activation was accelerated in the HFD-fed gerbils. Cytokines including interleukin-2 and -4 were more sensitive in the hippocampal CA1 region of the HFD-fed gerbils after ischemia-reperfusion. Additionally, we found that decreased 4-HNE and SODs immunoreactivity and protein levels in the hippocampal CA1 region of the HFD-fed gerbils after ischemia-reperfusion. These results indicate that HFD may lead to the exacerbated effects on ischemia-induced neuronal death in the hippocampal CA1 region after ischemia-reperfusion. These effects of HFD may be associated with more accelerated activations of glial cells and imbalance of pro- and anti-inflammatory cytokines and/or antioxidants after transient cerebral ischemia.

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Acknowledgments

The authors would like to thank Mr. Seung Uk Lee for his technical help in this study. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and future Planning (NRF-2014R1A2A2A01005307), and by the National Research Foundation of Korea (NRF-2013M3A9B6046563), which was funded by the Ministry of Science, ICT, and Future Planning.

Conflict of interest

The authors have no financial conflict of interest.

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, 225001, China

    Bing Chun Yan

  2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China

    Bing Chun Yan

  3. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea

    Joon Ha Park, Ji Hyeon Ahn, In Hye Kim, Jae-Chul Lee & Moo-Ho Won

  4. Department of Oral Anatomy, College of Dentistry, Research Institute of Oral Sciences, Gangneung-Wonju National University, Gangneung, 210-702, South Korea

    Ki-Yeon Yoo

  5. Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon, 200-701, South Korea

    Jung Hoon Choi

  6. Department of Anatomy and Cell Biology, College of Veterinary Medicine, Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea

    In Koo Hwang

  7. Department of Emergency Medicine, Institute of Medical Sciences, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea

    Jun Hwi Cho

  8. Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, 120-749, South Korea

    Young-Guen Kwon

  9. Department of Molecular and Cellular Biochemistry, Vascular System Research Center, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea

    Young-Myeong Kim

  10. Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, 330-714, South Korea

    Choong Hyun Lee

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  1. Bing Chun Yan
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  2. Joon Ha Park
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Corresponding authors

Correspondence to Choong Hyun Lee or Moo-Ho Won.

Additional information

Bing Chun Yan and Joon Ha Park have contributed equally to this article.

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Yan, B.C., Park, J.H., Ahn, J.H. et al. Effects of High-Fat Diet on Neuronal Damage, Gliosis, Inflammatory Process and Oxidative Stress in the Hippocampus Induced by Transient Cerebral Ischemia. Neurochem Res 39, 2465–2478 (2014). https://doi.org/10.1007/s11064-014-1450-8

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  • DOI: https://doi.org/10.1007/s11064-014-1450-8

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