Abstract
Regulators of mitogen activated protein kinases (MAPK) and c-Jun N-terminal/stress-activated kinase (JNK) include Rho-like small GTP-binding proteins and their regulators. SynGAP and kalirin-7 are postsynaptic density-enriched proteins identified through their interaction with Rho GTPases and PSD-95 scaffold protein. We examined immunoreactivity of SynGAP, kalirin-7, and PSD-95, phosphorylation of MAPK and JNK in control and postischemic hippocampus in gerbil model of transient forebrain ischemia. In normal brain higher amount of kalirin-7 but a lower amount of P-JNK was found in ischemia-resistant hippocampal area: CA2-3, DG than in ischemia-vulnerable CA1. After 5 min ischemia and 1 h reperfusion a decrease of P-ERK and increase of P-JNK were uniformly observed in the hippocampal parts. By contrast, the amount of kalirin-7 in CA2-3, DG reached 56% (P < 0.001) of control while was doubled in CA1. Oppositely, the immunoreactivity of SynGAP was increased in CA2-3, DG and reduced in CA1. Our data indicate that SynGAP and kalirin-7 take part in the regulation of ischemic signal transduction but the mechanism does not seem directly connected with the activation of MAPK and JNK.
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The project was sponsored by the Polish Ministry of Science and Higher Education 2P04A 024 28 and statutory grant to Mossakowski Medical Research Centre.
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Beręsewicz, M., Kowalczyk, J.E. & Zabłocka, B. Kalirin-7, a Protein Enriched in Postsynaptic Density, is Involved in Ischemic Signal Transduction. Neurochem Res 33, 1789–1794 (2008). https://doi.org/10.1007/s11064-008-9631-y
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DOI: https://doi.org/10.1007/s11064-008-9631-y