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Variation in reproductive phenology in a Pinus patula seed orchard and risk of genetic contamination from nearby natural stands

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Abstract

Genetic variation in reproductive phenology among clones in a seed orchard affects the genetic efficiency of the orchard. Our objective was to evaluate genetic variation in reproductive phenology of Pinus patula clones in a seed orchard and the amount of overlap with pollen dispersal in natural stands. In 2014 and 2015, phenology of female and male strobili was recorded on 31 clones in the orchard, and phenology of male strobili was measured on 10 trees in each of four nearby natural stands along an elevational transect. Onset and end dates, and length of female receptivity (F_onset, F_end and F_length) and pollen dispersal (M_onset, M_end and M_length) were calculated, and genetic parameters were estimated. Differences between years in M_onset were larger in the natural stands than in the orchard, but there was a large overlap between the orchard and natural stands. A negative linear relationship with elevation was found for M_end and M_length in natural stands along the elevation transect. Genetic variation was detected for most reproductive phenology traits in the orchard. Genetic control was stronger for M_onset and M_length (\(H_{c}^{2}\) ≥ 0.54) than for female receptivity traits (\(H_{c}^{2}\) ≤ 0.38). Most phenological traits showed high genetic stability in both years (rB ≥ 0.76). We found a positive genetic correlation (r = 0.67) between F_onset and M_onset, suggesting there is a risk of selfing among clonal ramets. Moreover, the overlap between female receptivity in the orchard and pollen dispersal in neighboring natural stands indicates a risk of genetic contamination in the orchard, particularly for late-phenology clones.

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References

  • Adams WT, Birkes DS (1989) Mating patterns in seed orchards. In: Proceedings of the 20th southern forest tree improvement conference, Charleston South Carolina, pp 75–86

  • Adams WT, Burczyk J (2000) Magnitude and implications of gene flow in gene conservation reserves. In: Young A, Boshier D, Boyle T (eds) Forest conservation genetics: principles and practice. CSIRO Publishing, Collingwood, pp 215–224

    Chapter  Google Scholar 

  • Alizoti PG, Kilimis K, Gallios P (2010) Temporal and spatial variation on flowering among Pinus nigra Arn. clones under changing climatic conditions. For Ecol Manag 259:786–797. https://doi.org/10.1016/j.foreco.2009.06.029

    Article  Google Scholar 

  • Askew GR (1988) Estimation of gamete pool compositions in clonal seed orchards. Silvae Genet 37:227–232

    Google Scholar 

  • Askew GR, Blush TD (1990) An index of phenological overlap in flowering for clonal conifer seed orchards, short note. Silvae Genet 39:168–171

    Google Scholar 

  • Blush TD, Bramlett DL, El-Kassaby YA (1993) Reproductive phenology of seed orchards. In: Bramlett DL, Askew GR, Blush TD, Bridgwater FE, Jett JB (eds) Advances in Pollen Management, USDA, Forest Service, Agriculture Handbook 698, pp 15–23

  • Boes TK, Brandle JR, Lovett WR (1991) Characterization of flowering phenology and seed yield in a Pinus sylvestris clonal seed orchard in Nebraska. Can J For Res 21:1721–1729. https://doi.org/10.1139/x91-238

    Article  Google Scholar 

  • Burczyk J, Chalupka W (1997) Flowering and cone production variability and its effect on parental balance in a Scots pine clonal seed orchard. Ann For Sci 54:129–144. https://doi.org/10.1051/forest:19970201

    Article  Google Scholar 

  • Castaños-Martínez LJ, Castro-Zavala S (2014) Manejo forestal: Reserva Forestal Multifuncional “El Manantial” S.C. Conceptos, Conductas y Acciones. Comisión Nacional Forestal. Zapopan, Jalisco, México, 222 p

  • Chaisurisri K, El-Kassaby YA (1993) Estimation of clonal contribution to cone and seed crops in a Sitka spruce seed orchard. Ann For Sci 50:461–467. https://doi.org/10.1051/forest:19930504

    Article  Google Scholar 

  • Codesido V, Merlo E (2001) Caracterización fenológica del huerto semillero de Pinus radiata de Sergude III Congreso Forestal Español. Actas del Congreso Tomo III, pp 69–74

  • Codesido V, Merlo E, Fernández-López J (2005) Variation in reproductive phenology in a Pinus radiata D. Don seed orchard in Northern Spain. Silvae Genet 54:246–256. https://doi.org/10.1515/sg-2005-0035

    Article  Google Scholar 

  • Dick JM, Leakey RRB, Jarvis PG (1990) Influence of female cones on the vegetative growth of Pinus contorta trees. Tree Physiol 6:151–163. https://doi.org/10.1093/treephys/6.2.151

    Article  CAS  PubMed  Google Scholar 

  • Di-Giovanni P, Kevan PG (1991) Factors affecting pollen dynamics and its importance to pollen competition: a review. Can J For Res 21:1155–1170. https://doi.org/10.1139/x91-163

    Article  Google Scholar 

  • Ebell LF, Schmidt RL (1964) Meteorological factors affecting conifer pollen dispersal on Vancouver Island. Department of Forestry, Forest Entomology and Pathology Branch. Government of Canada, Ottawa. Department of Forestry Publication No. 1036, 34 p

  • El-Kassaby YA, Askew GR (1991) The relation between reproductive phenology and reproductive output in determining the potential gametic pool profile in a Douglas-fir seed orchard. For Sci 37:827–835. https://doi.org/10.1093/forestscience/37.3.827

    Article  Google Scholar 

  • El-Kassaby YA, Reynolds S (1990) Reproductive phenology, parental balance, and supplemental mass pollination in a Sitka-Spruce seed orchard. For Ecol Manag 31:45–54. https://doi.org/10.1016/0378-1127(90)90110-W

    Article  Google Scholar 

  • El-Kassaby YA, Ritland K (1986) The relationship of outcrossing and contamination to reproductive phenology and supplemental mass pollination in a Douglas-fir seed orchard. Silvae Genet 35:240–244

    Google Scholar 

  • El-Kassaby YA, Fashler AMK, Sziklai O (1984) Reproductive phenology and its impact on genetically improved seed production in a Douglas-fir seed orchard. Silvae Genet 33:120–125

    Google Scholar 

  • El-Kassaby YA, Rudin D, Yazdani R (1989) Levels of outcrossing and contamination in two Pinus sylvestris L. seed orchards in northern Sweden. Scand J For Res 4:41–49. https://doi.org/10.1080/02827588909382544

    Article  Google Scholar 

  • Eriksson VJ, Jonsson A, Lindgren D (1973) Flowering in a clonal trial of Picea abies Karst. Stud For Suec 110:5–45

    Google Scholar 

  • Falconer DS (1989) Introduction to quantitative genetics, 4th edn. Longman, New York

    Google Scholar 

  • Griffin AR (1980) Isolation of a radiata pine seed orchard from external pollen. Aust For Res 10:83–94

    Google Scholar 

  • Griffin AR (1984) Clonal variation in radiata pine seed orchards II Flowering phenology. Aust For Res 14:271–281

    Google Scholar 

  • Harju AM, Nikkanen T (1996) Reproductive success of orchard and nonorchard pollens during different stages of pollen shedding in a Scots pine seed orchard. Can J For Res 26:1096–1102. https://doi.org/10.1139/x26-121

    Article  Google Scholar 

  • Hernández-Zaragoza O, López-Upton J, Vargas-Hernández JJ, Jiménez-Casas M (2016) Variación clonal de la fenología reproductiva en un huerto semillero de Pinus patula. Bosque 37:255–264. https://doi.org/10.4067/S0717-92002016000200004

    Article  Google Scholar 

  • Kang KS, Lindgren D, Mullin TJ (2001) Prediction of genetic gain and gene diversity in seed orchards crops under alternative management strategies. Theor Appl Genet 103:1099–1107. https://doi.org/10.1007/s001220100700

    Article  Google Scholar 

  • Kaya N, Isik K, Adams WT (2006) Mating system and pollen contamination in a Pinus brutia seed orchard. New For 31:409–416. https://doi.org/10.1007/s11056-005-0876-x

    Article  Google Scholar 

  • Khanduri PV (2012) Temporal and spatial variation of pollen yield in natural populations of Pinus roxburghii. For Stud China 14:20–29. https://doi.org/10.1007/s11632-012-0107-4

    Article  Google Scholar 

  • Lario FJ, Merlo E, Peñuelas JL, Gil L (2001) Variabilidad clonal de la fenología reproductiva y producción floral. Participación clonal en un huerto semillero de Pinus nigra Arnold salzmannii (Dunal) Franco. Actas del III Congreso Forestal Español. Granada, España. pp 539–545

  • Lindgren D, Paule L, Xihuan S, Yazdani R, Segerström ULF, Wallin JE, Lejdebro ML (1995) Can viable pollen carry Scots pine genes over long distances? Grana 34:64–69. https://doi.org/10.1080/00173139509429035

    Article  Google Scholar 

  • Luomajoki A (1993) Climatic adaptation of Scots pine (Pinus sylvestris L.) in Finland based on male flowering phenology. Acta For Fenn 237:1–27. http://hdl.handle.net/1975/9343

  • Matziris D (1994) Genetic variation in the phenology of flowering in Black pine. Silvae Genet 43:321–328

    Google Scholar 

  • Miguel-Pérez I, González-Martínez SC, Alía-Miranda R, Gil-Sánchez L (2002) Growth phenology and mating system of maritime pine (Pinus pinaster Aiton) in central Spain. Invest Agr Sist Recur For 11:193–204

    Google Scholar 

  • Mohan SC, Khanduri VP, Ghildiyal SK (2012) Reproductive ecology of male and female strobili and mating system in two different populations of Pinus roxburghii. Sci World J. https://doi.org/10.1100/2012/271389

    Article  Google Scholar 

  • Moriguchi Y, Taira H, Tsumura Y (2007) Paternal gene flow in Crytomeria japonica seed orchards as revealed by analysis of microsatellite markers. In: Lindgren D (ed) Proceedings of a Seed Orchards Conference, Umea, Sweden, pp 183–188

  • Muñoz-Gutiérrez L, Vargas-Hernández JJ, López-Upton J, Ramírez-Herrera C, Jiménez-Casas M, Aldrete A, Díaz-Ruíz R (2017) Variación especial y temporal en la dispersión de polen en un huerto semillero y en rodales naturales cercanos de Pinus patula. Bosque 38:169–181. https://doi.org/10.4067/S0717-92002017000100017

    Article  Google Scholar 

  • Nikkanen T (2001) Reproductive phenology in a Norway spruce seed orchard. Silva Fenn 35:39–53 https://doi.org/10.14214/sf.602

    Article  Google Scholar 

  • Nikkanen T, Velling P (1987) Correlations between flowering and some vegetative characteristics of grafts of Pinus sylvestris. For Ecol Manag 19:35–40. https://doi.org/10.1016/0378-1127(87)90009-0

    Article  Google Scholar 

  • Pakkanen A, Pulkkinen P (1991) Pollen production and background pollination levels in Scots pine seed orchard of Northern Finnish origin. In: Lindgren D (ed) Pollen contamination in seed orchards. Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences. Report 10:14–21

  • Pakkanen A, Nikkanen T, Pulkkinen P (2000) Annual variation in pollen contamination and outcrossing in a Picea abies seed orchard. Scand J For Res 15:399–404. https://doi.org/10.1080/028275800750172574

    Article  Google Scholar 

  • Parantainen A, Pulkkinen P (2003) Flowering and airbone pollen occurrence in a Pinus sylvestris seed orchard consisting of northern clones. Scand J For Res 18:111–117. https://doi.org/10.1080/028275800310003687

    Article  Google Scholar 

  • Pulkkinen P (1994) Aerobiology of pine pollen: dispersal of pollen from non-uniform sources and impact on Scots pine seed orchard. Reports from the foundation for forest tree breeding 8:1–23

  • Reynolds S, El-Kassaby YA (1990) Parental balance in Douglas-fir seed orchards (cone crop versus seed crop). Silvae Genet 39:40–42

    Google Scholar 

  • SAS (Statistical Analysis System) Institute (2002) SAS/STAT Computer Software. Release 9.00. SAS Institute Inc. Cary. N.C. 328 p

  • Silen RR (1963) Effect of altitude on factors of pollen contamination of Douglas-fir seed orchards. J For 61:281–283

    Google Scholar 

  • Slavov GT, Howe GT, Adams WT (2005) Pollen contamination and mating patterns in a Douglas-fir seed orchard as measured by simple sequence repeat markers. Can J For Res 35:1592–1603. https://doi.org/10.1139/X05-082

    Article  CAS  Google Scholar 

  • Stewart SC (1994) Simultaneous estimation of pollen contamination and pollen fertilities of individual trees in conifer seed orchards using multilocus genetic data. Theor Appl Genet 88:593–596. https://doi.org/10.1007/BF01240923

    Article  CAS  PubMed  Google Scholar 

  • Torimaru T, Wennström U, Andersson B, Almqvist C, Wang XR (2013) Reduction of pollen contamination in Scots pine seed orchard crop by tent isolation. Scand J For Res 28:715–723. https://doi.org/10.1080/02827581.2013.838298

    Article  Google Scholar 

  • White TL, Hodge GR (1989) Predicting breeding values with applications in forest tree improvement. Kluwer Academic Publishers, The Netherlands, p 368

    Book  Google Scholar 

  • Zas R, Merlo E, Fernández-López J (2003) Synchro: a SAS program for analyzing the floral phenological synchronization in seed orchards. Silvae Genet 52:212–215

    Google Scholar 

  • Zhang X, Ren J, Zhang D (2001) Phenological observations on Larix principis-rupprechtii Mayr in primary seed orchard. J For Res 12:201–204. https://doi.org/10.1007/BF02856707

    Article  Google Scholar 

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Acknowledgements

The authors are grateful to Ing. León Jorge Castaños Martínez and Salvador Castro Zavala, owners of “Reserva Forestal Multifuncional El Manantial” for giving us access and logistic support to obtain the field data. We also thank to the National Council of Science and Technology (CONACyT) in Mexico for the scholarship provided to Liliana Muñoz-Gutiérrez during her Ph. D. studies, and to the Colegio de Postgraduados for the financial support to carry out the study. We appreciate the valuable comments received from two anonymous reviewers to improve the manuscript.

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  1. Colegio de Postgraduados, Campus Montecillo, Km. 36.5, Carretera México-Texcoco, Montecillo, Estado de México, 56230, Mexico

    Liliana Muñoz-Gutiérrez, J. Jesús Vargas-Hernández, Javier López-Upton, Carlos Ramírez-Herrera, Marcos Jiménez-Casas & Arnulfo Aldrete

  2. CENID COMEF, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Av. Progreso Núm. 5, Barrio de Santa Catarina, Alcaldia Coyoacán, Ciudad de México, 04010, Mexico

    Liliana Muñoz-Gutiérrez

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  1. Liliana Muñoz-Gutiérrez
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Correspondence to J. Jesús Vargas-Hernández.

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Muñoz-Gutiérrez, L., Vargas-Hernández, J.J., López-Upton, J. et al. Variation in reproductive phenology in a Pinus patula seed orchard and risk of genetic contamination from nearby natural stands. New Forests 50, 1027–1041 (2019). https://doi.org/10.1007/s11056-019-09712-1

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