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Mating System and Pollen Contamination in a Pinus brutia Seed Orchard

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Abstract

Mating system parameters and pollen contamination were estimated in an 11-year-old Pinus brutia Ten. clonal seed orchard, located in Antalya, Turkey, with the aid of isozymes. Isozyme analysis was performed on both maternal (megagametophyte) and embryo tissues of seeds collected from the seed orchard and the surrounding natural stand. Fourteen loci encoding nine enzyme systems were assayed. Based on a multilocus estimator, the proportion of viable seeds originating from outcrossing (tˆ m ) in the seed orchard was found to be 0.947; the remainder (0.053) was due to selfing. We estimated that 85.7% of the orchard seed resulted from pollen contamination by trees in surrounding stands. This may be related to the young age and subsequent low pollen production of the orchard. Only 9% [i.e., 1.0−0.857 (contamination)−0.053 (selfing) = 0.090] of the orchard offspring was due to cross-pollination among clones within the orchard. Due to the high level of pollen contamination, it is estimated that genetic gain in this seed crop is less than 57% of that expected if all offspring resulted from random mating among orchard clones (i.e., no contamination).

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References

  • Adams W.T. and Birkes D.S. 1989. Mating patterns in seed orchards. In: Proceedings of 20th Southern Forest Tree Improvement Conference. June 26–30, 1989. Charleston, South Carolina.

  • W.T. Adams D.S. Birkes (1991) Estimating mating patterns in forest tree populations S. Fineschi M.E. Malvoltti F. Cannata H.H. Hattemer (Eds) Biochemical Markers in the Population Genetics of Forest Trees Spb Academic Publishing Bv The Hague,The Netherlands 157–172

    Google Scholar 

  • W.T. Adams J. Burczyk (2000) Magnitude and implications of gene flow in gene conservation reserves A. Young D. Boshier T. Boyle (Eds) Forest Conservation Genetics: Principles and Practice CSIRO Publishing, Collingwood Victoria, Australia 215–224

    Google Scholar 

  • W.T. Adams J. Burczyk (1993) Genflow: A Computer Program for Estimating Levels of Pollen Contamination in Clonal Seed Orchards Release 1. Dept. of Forest ScienceOregon State Univ. Corvallis, OR, USA

    Google Scholar 

  • Adams W.T., Birkes D.S. and Erickson V.J. 1992. Using genetic markers to measure gene flow and pollen dispersal in forest tree seed orchards. In: Wyatt R.Ecology and Evolution of Plant Reproduction, pp. 37–61.

  • W.T. Adams V.D. Hipkins J. Burczyk W.K. Randall (1997) ArticleTitlePollen contamination trends in a maturing Douglas-fir seed orchard Can. J. For. Res. 27 131–134 Occurrence Handle10.1139/cjfr-27-1-131

    Article  Google Scholar 

  • J. Burczyk W.T. Adams J.Y. Shimizu (1996) ArticleTitleMating patterns and pollen dispersal in a natural knobcone pine (Pinus attenuata Lemmon.) Stand Heredity 77 251–260 Occurrence Handle10.1038/sj.hdy.6880410

    Article  Google Scholar 

  • J. Burczyk W.T. Adams J.Y. Shimizu (1997) ArticleTitleMating system and genetic diversity in natural populations of knobcone pine (Pinus attenuata) Forest Genet. 4 IssueID4 223–226

    Google Scholar 

  • Conkle M.T., Hodgskiss P.D., Nunnally L.B. and Hunter S.C. 1982. Starch Gel Electrophoresis of Conifer Seeds: A Laboratory Manual U.S.D.A. Gen. Techn. Rept. PSW:64, 18 pp.

  • F. Di-Giovanni P.G. Kevan (1991) ArticleTitleFactors affecting pollen dynamics and its importance to pollen contamination: a review Can. J. For. Res. 21 1155–1170

    Google Scholar 

  • Y.A. El-Kassaby M.D. Meagher J. Parkinson F.T. Portlock (1987) ArticleTitleAllozyme inheritanceheterozygosity and outcrossing rate among Pinus Monticola near Ladysmith British Columbia Heredity 58 173–181

    Google Scholar 

  • Y.A. El-Kassaby D. Rudin R. Yazdani (1989) ArticleTitleLevels of outcrossing and contamination in two Pinus Sylvestris L. seed orchards in Northern Sweden Scand. J. For. Res. 4 41–49

    Google Scholar 

  • V.J. Erickson W.T. Adams (1990) ArticleTitleMating system variation among individual ramets in a Douglas-fir seed orchard Can. J. For. Res. 20 1672–1675

    Google Scholar 

  • W. Fast B.P. Dancik R.C. Bower (1986) ArticleTitleMating system and pollen contamination in a Douglas-fir clone bank Can. J. For. Res. 16 1314–1319

    Google Scholar 

  • S.T. Friedman W.T. Adams (1985) ArticleTitleEstimation of gene flow into two seed orchards of Lobolly pine (Pinus taeda L.) Theor. Appl. Genet. 69 609–615 Occurrence Handle10.1007/BF00251112

    Article  Google Scholar 

  • Greenwood M.S. and Rucker T. 1985. Estimating pollen contamination in Loblolly pine seed orchards by pollen trapping. Proc. 18th Southern Forest Tree Improv. Conf., May 21–23, 1985.

  • A. Harju O. Muona (1989) ArticleTitleBackground pollination in Pinus sylvestris seed orchards Scand. J. For. Res. 4 513–520

    Google Scholar 

  • N. Kara L. Korol K. Isik G. Schiller (1997) ArticleTitleGenetic diversity in Pinus brutia Ten.: altitudinal variation Silvae Genet. 46 IssueID2–3 155–161

    Google Scholar 

  • N. Kaya (2001) Estimation of the Mating System and Genetic Contamination in a Clonal Seed Orchard of Pinus brutia Ten. in Asar-Antalya Akdeniz Univ. Antalya, Turkey 81

    Google Scholar 

  • K.V. Krutovskii D.V. Politov Y.P. Altukov (1995) Isozyme study of population genetic structuremating system and phylogenetic relationships of the five stone pine species (subsection Cembraesection Strobisubgenus Strobus) Ph. Baradat W.T. Adams G. Muller-Starck (Eds) Population Genetics and Genetic Conservation of Forest Trees Spb Academic Publishing AmsterdamThe Netherlands 279–304

    Google Scholar 

  • P. Ladislav D. Gömory (1995) Mating system in the seed orchards of European Larch (Larix decidua Mill) Ph. Baradat W.T. Adams G. Muller-Starck (Eds) Population Genetics and Genetic Conservation of Forest Trees SPB Academic Publishing Amsterdam, The Netherlands 321–328

    Google Scholar 

  • P. Ladislav D. Lindgren R. Yazdani (1993) ArticleTitleAllozyme frequencies, outcrossing rate and pollen contamination in Picea abies seed orchards Scand. J. For. Res. 8 8–17

    Google Scholar 

  • Nagasaka K. and Szmidt A.E. 1985. Multilocus analysis of extarnal pollen contamination of a Scots pine (Pinus sylvestris L.) seed orchard. In: Gregorius H.R.Population Genetics in Forestry. Lecture Notes in Biomathematics. 60: 134–138.

  • D.B. Neale W.T. Adams (1985) ArticleTitleThe mating system in natural and shelterwood stands of Douglas-fir Theor. Appl. Genet. 71 201–207

    Google Scholar 

  • S.K. Omi W.T. Adams (1985) ArticleTitleVariation in seed set and proportions of outcrossed progeny with clones, crown position, and top pruning in a Douglas-fir seed orchard Can. J. For. Res. 16 502–507

    Google Scholar 

  • Pakkanen A. and Pulkinen P. 1991. Pollen production and background pollination levels in Scots pine seed orchards of Northern Finnish origin. In: Lindgren D.Proceedings of The Meeting of The Nordic Group For Tree Breeding 1991. Swedish Univ. of Agricultural Sciences, Dept. of Forest Genetics and Plant Physiology Umeå Rep.10, pp. 14–21.

  • K. Ritland (1998) Mltr: Multilocus Mating System ProgramVersion 1.0 Dept. of Forst Sciences, Univ. of British Columbia Vancouver, Canada

    Google Scholar 

  • K. Ritland Y.A. El-Kassaby (1985) ArticleTitleThe nature of inbreeding in a seed orchard of Douglas-fir as shown by an efficient multilocus model Theor. Appl. Genet. 71 375–384 Occurrence Handle10.1007/BF00251176

    Article  Google Scholar 

  • SmithD.B., Adams and W.T. 1983. Measuring pollen contamination in clonal seed orchards with the aid of genetic markers. In: Proceedings, 17th Southern Forest Tree Improvement Conference; 1983 June 6–9. Athens pp. 69–77.

  • R.A. Sniezko (1981) Genetic and economic consequences of pollen contamination in seed orchards Proceedings, 16th Southern Forest Tree Improvement Conference; 1981 May 27–28 Virginia Polytechnic Institute Blacksburg, VA, Blacksburg

    Google Scholar 

  • A.E. Squillace E.M. Long (1981) Proportion of pollen from nonorchard sources E.C. Franklin (Eds) Pollen Management Handbook USDA Agric. Handb. 587 Washington, DC 15–19

    Google Scholar 

  • X. Wang D. Lindgren A.E. Szmidt R. Yazdani (1991) ArticleTitlePollen migration into a seed orchard of Pinus sylvestris L. and the methods of its estimation using allozyme markers Scand. J. For. Res. 6 379–385 Occurrence Handle10.1080/02827589109382675

    Article  Google Scholar 

  • Wheeler N. and Jech K. 1986. Pollen contamination in a matureDouglas-fir seed orchard. Proc. Iufro Conf. Breeding, Theory, Progeny Testing of Seed Orchards. Oct. 13–17, 1986. VA, Williamsburg.

  • Wiselogel A.E. 1986. Pollen contamination in a superior Loblolly pine seed orchard. Proc. Ninth North Amer. For. Biol. Workshop. June 15–18. Stillwater OK, USA pp. 274–278.

  • B.J. Zobel J. Barber C.L. Brown T.O. Perry (1958) ArticleTitleSeed orchards – Their concept and management J. Forest. 56 815–825

    Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Faculty of Arts and Sciences, Akdeniz University, 07058, Antalya, Turkey

    Nuray Kaya & Kani Isik

  2. Department of Forest Science, Oregon State University, Corvallis, OR , 97331, USA

    W. T. Adams

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  1. Nuray Kaya
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  2. Kani Isik
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  3. W. T. Adams
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Correspondence to Nuray Kaya.

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Kaya, N., Isik, K. & Adams, W.T. Mating System and Pollen Contamination in a Pinus brutia Seed Orchard. New Forest 31, 409–416 (2006). https://doi.org/10.1007/s11056-005-0876-x

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