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Application of upconversion luminescent-magnetic microbeads with weak background noise and facile separation in ochratoxin A detection

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Abstract

Ochratoxin A (OTA), the most harmful and abundant ochratoxin, is chemically stable and commonly existed in foodstuffs. In this work, upconversion luminescent-magnetic microbeads (UCLMMs) -based cytometric bead array for OTA detection with a less reagent consumption and high sensitivity has been established and optimized. In UCLMMs, upconversion nanocrystals (UCNs) for optical code present a weak background noise and no spectral cross talk between the encoding signals and target labels under two excitation conditions to improve detection sensitivity. While the superparamagnetic Fe3O4 nanoparticles (Fe3O4 NPs) aim for rapid analysis. The results show that the developed method has a sensitivity of 9.553 ppt below HPLC with a 50-μL sample and can be completed in <2 h with good accuracy and high reproducibility. Therefore, different colors of UCLMMs will become a promising assay platform for multiple mycotoxins after further improvement.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31401578, 51373117, 51303126, 51573128, and 81402575) and Tianjin Natural Science Foundation (13JCZDJC33200 and 15JCQNJC03100).

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    Authors and Affiliations

    1. The National Center of Supervision and Inspection for Quality of Food, Tianjin Product Quality Inspection Technology Research Institute, Tianjin, 300384, People’s Republic of China

      Zhenyu Liao

    2. School of Life Sciences, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

      Ying Zhang, Jin Chang & Hanjie Wang

    3. Eye Institute and School of Optometry and Ophthalmology, Tianjin Medical University Eye Hospital, Tianjin, 300384, People’s Republic of China

      Lin Su

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    1. Zhenyu Liao
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    Correspondence to Zhenyu Liao or Hanjie Wang.

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    Zhenyu Liao, Ying Zhang, Lin Su, Jin Chang, and Hanjie Wang declare that they have no conflict of interest.

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    Zhenyu Liao and Ying Zhang contributed equally to this work.

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    Liao, Z., Zhang, Y., Su, L. et al. Application of upconversion luminescent-magnetic microbeads with weak background noise and facile separation in ochratoxin A detection. J Nanopart Res 19, 60 (2017). https://doi.org/10.1007/s11051-016-3717-2

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